Summary
The reactivity was examined of horseradish peroxidase labelledUlex europaeus agglutinin-I (UEA-I) andGriffonia simplicifolia agglutinin I-B4 (GSAI-B4) with red blood cells and vascular endothelium in formalin-fixed, paraffin embedded tissues from 18 primate species. The expression of blood group ABH antigens in these cells as well as secretions from other tissues was also examined by the indirect immunoperoxidase method using monoclonal anti-ABH antibodies as primary antibodies. In Prosimians and New World monkeys which lack ABH antigens on both red blood cells and endothelial cells, but produce these antigens in other tissue secretions, GSAI-B4 always reacted with both red blood cells and endothelial cells. In Old World monkeys, which express blood group antigens on endothelial cells but not on red blood cells, neither GSAI-B4 nor UEA-I reactivity were observed, except the endothelial cells from blood group B or O individuals occasionally reacted with GSAI-B4 or UEA-I, respectively. Although UEA-I reactivity was not observed in the endothelial cells of gibbon, it reacted with these cells from chimpanzees. In these two anthropoid apes, both endothelial cells and red blood cells expressed ABH antigens as in humans. These results suggest the close evolutionary relationship between the expression of blood group ABH antigens and lectin binding properties of red blood cells and endothelial cells in primate species.
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ALROY, J., GOYAL, V. & SKUTELSKY, E. (1987) Lectin histochemistry of mammalian endothelium.Histochemistry 86, 603–7.
HENNIGAR, R. H., DONALD, A. S., OTHERSEN, H. B. & GARRVIN, A. J. (1988) Distribution of fucosubstance in kidney and related neoplasms. Absence of lectin-reactive α-fucose from vasculature of bilateral Wilm's tumours.Arch. Pathol. Lab. Med. 112, 908–13.
HOLTHÖFER, H., VIRTANEN, I., KARINIEMI, A-L., HORMIA, M., LINDER, E. & MIETTINEN, A. (1982)Ulex europaeus lectin I as a marker for vascular endothelium in human tissues.Lab Invest. 47, 60–6.
HOLTHÖFER, H. (1983) Lectin binding sites in kidney. A comparative study of 14 animal species.J. Histochem. Cytochem. 31, 531–7.
ITO, N., NISHI, K., NAKAJIMA, M., MATSUDA, Y., ISHITANI, A., MIZUMOTO, J. & HIROTA, T. (1986) Localization of blood group antigens in human pancreas with lectin-horseradish peroxidase conjugates.Acta Histochem. Cytochem. 19, 205–18.
ITO, N., NISHI, K., NAKAJIMA M., ISHITANI, A., OKAMURA, Y., MATSUDA, Y. & HIROTA, T. (1987) Histochemical reactivity of soybean agglutinin with blood group antigens and their precursor substances in acinar cells of human pancreas.J. Histochem. Cytochem. 35, 881–90.
LAITINEN, L. (1987)Griffonia simplicifolia lectins bind specifically to endothelial cells and some epithelial cells in mouse tissues.Histochem J. 19, 225–34.
NISHI, K., FECHNER, G., RAND, S. & BRINKMANN, B. (1989) Light-microscopic examination of ABH and Lewis antigens in human tracheal and epiglottic glands using the avidin-biotin-peroxidase complex technique.Z. Rechtmed. 102, 255–62.
ORIOL, R., COOPER, J. E., DAVIES, D. R. & KEELING, P. W. N. (1984) ABH antigens in vascular endothelium and some epithelial tissues of baboons.Lab. Invest. 50, 514–18.
PEREIRA, M. E. A. & KABAT, E. A. (1974) Specificity of purified hemagglutinin (lectin) fromLotus tetragonolobus.Biochemistry 13, 3184–92.
PEREIRA, M. E. A., KISALIUS, E. C., GRUEZO, F. & KABAT, E. A. (1978) Immunochemical studies on the combining site of the blood group H-specific lectin I fromUlex europeus seeds.Arch. Biochem. Biophys. 185, 108–15.
PETRYNIAK, J. & GOLDSTEIN, I. J. (1986) Immunochemical studies on the interaction between synthetic glycoconjugates and α-l-fucosyl binding lectins.Biochemistry 25, 2829–38.
SOCHA, W. W., MARBOE, C. C., MICHLER, R. E., ROSE, E. A. & MOOR-JANKOWSKI, J. (1987) Primate animal model for the study of ABO incompatibility of organ transplantation.Transplant. Proc. 19, 4448–55.
SZULMAN, A. E. (1960) The histological distribution of blood group substances A and B in man.J. Exp. Med. 111, 785–800.
WATKINS, W. M. (1966) Blood-group substances. In the ABO system the genes control the arrangement of sugar residues that determines blood-group specificity.Science 152, 172–81.
WOOD, C., KABAT, E. A., MURPHY, L. A. & GOLDSTEIN, I. J. (1979) Immunochemical studies of the combining sites of the two isolectins, A4 and B4, isolated fromBanderiraea simplicifolia.Arch Biochem. Biophys. 198, 1–11.
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Ito, N., Nishi, K., Nakajima, M. et al. Relationship between lectin binding properties and the expression of blood group ABH antigens in vascular endothelia and red blood cells from 18 primate species. Histochem J 22, 113–118 (1990). https://doi.org/10.1007/BF01885790
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DOI: https://doi.org/10.1007/BF01885790