Summary
Expression of IGF-I and IGF-II was studied in human breast cancer tissues by in situ hybridization. IGF-I mRNA was detected only in stromal cells adjacent to normal breast epithelial cells. Stromal cells associated with the tumor cells did not contain IGF-I, nor did malignant or benign breast epithelial cells. In contrast, IGF-II mRNA was found in both the malignant epithelial cells and their adjacent stromal cells. These data imply that stromal cells associated with breast epithelium may switch expression from IGF-I to IGF-II during breast cancer evolution. This appearance of IGF-II expression may identify cancer-associated stromal cells that have a fetal phenotype.
Similar content being viewed by others
References
Yee D, Paik S, Lebovic GS, Marcus RR, Favoni RF, Cullen KJ, Lippman ME, Rosen N: Analysis of insulin-like growth factor I gene expression in malignancy: evidence for a paracrine role in human breast cancer. Mol Endocrinol 3:509–517, 1989
Hogan B, Costantini F, Lacy E: Manipulating the Mouse Embryo — A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, 1986, p228
Cullen KJ, Smith HS, Hill S, Rosen N, Lippman ME: Growth factor mRNA expression by human breast fibroblasts from benign and malignant lesions. Cancer Res 51:4978–4985, 1991
Paik S, Rosen N, Perdue JF, You JM, Lippman ME, Yee D: Expression of IGF-II mRNA in fetal kidney and Wilms' tumor; an in-situ hybridization study. Lab Invest 61:522–526, 1989
Yee D, Cullen KJ, Paik S, Perdue J, Hampton B, Schwartz A, Lippman ME, Rosen N: Insulin-like growth factor II mRNA expression in human breast cancer. Cancer Res 48:6691–6696, 1988
Minniti CP, Kohn EC, Grubb JH, Sly WS, Oh Y, Muller HL, Rosenfeld RG, Helman LJ: The insulin-like growth factor II (IGF-II)/mannose-6-phosphate receptor mediates IGF-II-induced motility in human rhabdomyosarcoma cells. J Biol Chem 267:9000–9004, 1992
Natali PG, Nicotra MR, Bigotti A, Botti C, Castellani P, Risso AM, Zardi L: Comparative analysis of the expression of the extracellular matrix protein tenascin in normal human fetal, adult, and tumor tissues. Int J Cancer 47:811–816, 1991
Chaponnier C, Gabbiani G: Gelsolin modulation in epithelial and stromal cells of mammary carcinoma. Am J Pathol 134:597–603, 1989
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Paik, S. Expression of IGF-I and IGF-II mRNA in breast tissue. Breast Cancer Res Tr 22, 31–38 (1992). https://doi.org/10.1007/BF01833331
Issue Date:
DOI: https://doi.org/10.1007/BF01833331