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Enzymatic peptide synthesis by proline iminopeptidase

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Summary

Proline iminopeptidase was screened for the enzymatic synthesis of peptides containing Pro. The enzyme was purified from the best strainBacillus brevis, and the partially purified enzyme was used for peptide synthesis from Pro-benzylester and Phe. The dipeptide Pro-Phe was obtained in a 40% yield.

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Author notes

  1. Takashi Ohshiro

    Present address: Department of Biotechnology, Faculty of Engineering, Tottori University, 680, Tottori, Japan

  2. Takayuki Uwajima

    Present address: Makuhari Techno Garden, Novo Nordisk Bioindustry Ltd., CB-6 3 Nakase 1-chome, Chiba-shi, 261-01, Chiba, Japan

Authors and Affiliations

  1. Tokyo Research Laboratories, Kyowa Hakko Co., Ltd., 3-6-6, Asahimachi, Machida-shi, 194, Tokyo, Japan

    Takashi Ohshiro, Kenichi Mochida & Takayuki Uwajima

Authors
  1. Takashi Ohshiro
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  2. Kenichi Mochida
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  3. Takayuki Uwajima
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Ohshiro, T., Mochida, K. & Uwajima, T. Enzymatic peptide synthesis by proline iminopeptidase. Biotechnol Lett 14, 175–178 (1992). https://doi.org/10.1007/BF01023354

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  • DOI: https://doi.org/10.1007/BF01023354

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