Abstract
Electron transport of normal and photobleachedAnabaena cylindrica was studied using spectral and kinetic analyses of absorbance transients induced by single turnover flashes. Between 500 and 600 nm two positive bands (∼540 and ∼566 nm) and two negative bands (∼515 and ∼554 nm) were found. Absorbance changes at 515 and 540 nm were partly characterized. None of these absorbance changes represent an electrochromic shift. Absorbance changes at 554 and 566 nm correspond to the oxidation of cytochromef and the reduction of cytochromeb 563, respectively. We found a very slight 3-(3,4-dichlorophenyl)-1, 1-dimethylurea (DCMU) sensitivity of cytochromef in normal cells, while DCMU was completely ineffective for cytochromef reduction in photobleached cells. The absorbance change of cytochromeb 563 increased, while the absorbance change of cytochromef was smaller than in normal cells. The increased O2 evolution in photobleached cells and the negligible electron transport via cytochromef suggest the participation of other electron acceptor(s) in the electron-transport chain of photobleachedAnabaena cylindrica.
Similar content being viewed by others
References
Allen, M. B., and Arnon, D. I. (1955).Plant Physiol. 30, 366–372.
Almon, H., and Böhme, H. (1980).Biochim. Biophys. Acta 592, 113–120.
Amesz, I., and Visser, J. W. M. (1971).Biochim. Biophys. Acta 234, 62–69.
Anderson, I. M. (1981).FEBS Lett. 124, 1–10.
Aoki, M., Hirano, M., Takahashi, Y., and Katoh, S. (1983).Plant Cell Physiol. 24, 517–525.
Binder, A. (1982).J. Bioenerg. Biomembr. 14, 271–286.
Böhme, H., Pelzer, B., and Böger, P. (1980).Biochim. Biphys. Acta 592, 528–535.
Bradley, S., and Carr, N. G. (1976).J. Gen. Microbiol. 96, 175–184.
Haselkon, R. (1978).Annu. Rev. Plant Physiol. 29, 319–344.
Hirano, M., Satoh, K., and Katoh, S. (1980).Photosynth. Res. 1, 149–162.
Hirano, M., and Katoh, S. (1981).Photochem. Photobiol. 34, 637–643.
Hirano, M., Satoh, K., and Katoh, S. (1981).Biochim. Biophys. Acta 635, 476–487.
Ho, K. K., and Krogmann, D. W. (1982). InBotanical Monographs: The Biology of the Cyanobacteria (Carr, N. G., and Whitton, B. A., eds.), Blackwell, Oxford.
Horváth, G., Niemi, H. A., Droppa, M., and Faludi-Dániel, A. (1979).Plant Physiol. 63, 778–782.
Houchins, I. P., and Hind, G. (1983a).Biochim. Biophys. Acta 725, 138–145.
Houchins, J. P., and Hind, G. (1983b).Arch. Biochem. Biophys. 224, 272–282.
Kawamura, M., Mimuro, M., and Fujita, Y. (1979).Plant Cell Physiol. 20, 697–705.
Laczkó, I., and Barabás, K. (1981).Planta 153, 312–316.
Lockau, W. (1981).Arch. Microbiol. 128, 336–340.
Marsho, T. V., and Kok, B. (1980).Methods Enzymol. 69, 280–289.
Melis, A., and Brown, J. (1980).Proc. Natl. Acad. Sci. USA 77, 4712–4716.
Meyers, J., Graham, J-R., and Wang, R. T. (1980).Plant Physiol. 66, 1141–1143.
Murai, T., and Katoh, T. (1975).Plant Cell Physiol. Tokyo 16, 759–797.
Nolan, W. G., and Bishop, D. G. (1975).Arch Biochem. Biophys. 166, 323–329.
Padan, E. (1979).Annu. Rev. Plant Physiol. 30, 27–40.
Peschek, G. (1983).Biochem. J. 210, 269–272.
Sandmann, G., and Böger, P. (1980).Plant Sci. Lett. 17, 417–424.
Sörensen, L., and Halldal, P. (1977).Photochem. Photobiol. 26, 511–518.
Spiller, M. (1980).Plant Physiol. 66, 446–450.
Spurr, A. R. (1969).J. Ultrastruct. Res. 26, 31–43.
Wasserman, A. R. (1980).Methods Enzymol. 69, 181–202.
Witt, H. T. (1980).Biochim. Biphys. Acta 505, 355–427.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Barabás, K., Laczkó, I. Characterization of the photosynthetic electron transport chain in normal and photobleachedAnabaena cylindrica by flash spectroscopy. J Bioenerg Biomembr 17, 123–134 (1985). https://doi.org/10.1007/BF00744202
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00744202