Abstract
Purpose
This study aimed to investigate the relationship between loss of AT-rich interactive domain-containing protein 1A (ARID1A) expression and the prognosis of patients with oral squamous cell carcinoma (OSCC).
Methods
Seventy-five OSCC tissue specimens were immunohistochemically stained with a specific antibody to ARID1A. The immunoreactivity of ARID1A was then correlated with clinicopathological factors, including the prognosis of the patients.
Results
Non-tumorous oral squamous cells exhibited nuclear ARID1A staining, whereas the invasive OSCC cells showed different degrees of loss of ARID1A immunoreactivity. Little-to-no ARID1A immunoreactivity was found at the cancer invasion front in 20 of the 75 OSCC tissue specimens, with significant association with unfavorable patient outcomes. Notably, downregulation of ARID1A immunoreactivity was also related to lymphovascular involvement and poor outcome in patients with T1 and T2 OSCC without lymph node metastasis.
Conclusions
The results suggest that loss of ARID1A expression at the cancer invasion front might be related to tumor progression in many OSCCs.
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The need for written informed consent was waived by the Institutional Review Board of the Gifu University Graduate School of Medicine. Instead, the Institutional Review Board requested us to inform the patients that they could refuse the use of their tissue specimens for this present study, if they did not want to participate. The present study was conducted in accordance with the ethical standards of the Helsinki Declaration in 1975, after approval of the Institutional Review Board of the Gifu University Graduate School of Medicine (specific approval number: 28-524).
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41548_2018_3_MOESM1_ESM.jpg
Supplementary material 1 (JPEG 52 kb) Supplementary Fig. 1. Overall survival curves according to ARID1A immunoreactivity scores, not restricted at invasion front, in OSCC. The Kaplan–Meier method and differences in the survival rates were compared using the log-rank test for univariate survival analysis. The overall survival rate of patients in the ARID1A-negative group was lower than that of patients in the ARID1A-positive group (P = 0.106)
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Inoue, K., Hano, K., Bunai, K. et al. Loss of ARID1A, a component of the SWI/SNF chromatin remodeling complex, at the invasion front is related to poor outcomes in oral squamous cell carcinoma. Oral Cancer 2, 1–5 (2018). https://doi.org/10.1007/s41548-018-0003-2
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DOI: https://doi.org/10.1007/s41548-018-0003-2