Abstract
The majority of anticancer drugs are hydrophobic and there is a big challenge to deliver them effectively to its target. One efficient way to overcome the challenge is to encapsulate them in an amphiphilic nanocarrier. This study will focus on the encapsulation of palmitic acid (PL) in chitosan nanoparticles by a two-step method, i.e., oil-in-water emulsion/ionic gelation technique to prepare amphiphilic chitosan nanoparticles (ACNs). Due to the importance of physicochemical characteristics of ACNs especially nanoparticle size on cell uptake, the present work has focused on the evaluation of some parameters’ effect such as, sonication power and time, cross-linker and stabilizer concentrations on ACNs size. The success of PL encapsulation was confirmed by Fourier transform infrared spectroscopy and the effect of the parameters were evaluated by laser light scattering and scanning electron microscopy. It was observed that each parameter affected nanoparticle size and morphology with an optimum limit. Thus, this study confirms the importance of preparation parameters on the physicochemical characteristics of ACNs.
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Ajun W, Yan S, Li G, Huili L (2009) Preparation of aspirin and probucol in combination loaded chitosan nanoparticles and in vitro release study. Carbohydr Polym 75:566–574
Akiyoshi K, Sunamoto J (1996) Supramolecular assembly of hydrophobized polysaccharides. Supramol Sci 3:157–163
Anarjan N, Tan CP (2013) Effects of selected polysorbate and sucrose ester emulsifiers on the physicochemical properties of astaxanthin nanodispersions. Molecules 18:768–777
Bonferoni M, Sandri G, Dellera E, Rossi S, Ferrari F, Mori M, Caramella C (2014) Ionic polymeric micelles based on chitosan and fatty acids and intended for wound healing. Comparison of linoleic and oleic acid. Eur J Pharm Biopharm 87:101–106
Calvo P, Remuñan-López C, Vila-Jato JL, Alonso MJ (1997) Chitosan and chitosan/ethylene oxide-propylene oxide block copolymer nanoparticles as novel carriers for proteins and vaccines. Pharm Res 14:1431–1436
Dev A, Binulal NS, Anitha A, Nair SV, Furuike T, Tamura H, Jayakumar R (2010) Preparation of poly(lactic acid)/chitosan nanoparticles for anti-HIV drug delivery applications. Carbohydr Polym 80:833–838
Dubey RR, Parikh RH (2004) Two-stage optimization process for formulation of chitosan microspheres. AAPS PharmSciTech 5:20–28
Dumitriu S, Chornet E (1998) Inclusion and release of proteins from polysaccharide-based polyion complexes. Adv Drug Deliv Rev 31:223–246
El Fray M, Niemczyk A, Pabin-Szafko B (2012) Chemical Modification of Chitosan with Fatty Acids. Prog Chem Appl Chitin Deriv 17:29–36
Garland R, Wise M, Beaver M, Dewitt H, Aiken A, Jimenez J, Tolbert M (2005) Impact of palmitic acid coating on the water uptake and loss of ammonium sulfate particles. Atmos Chem Phys 5:1951–1961
Hirano S, Seino H, Akiyama Y, Nonaka I (1990) Chitosan: a biocompatible material for oral and intravenous administrations. In: Progress in biomedical polymers. Springer, pp 283–290
Kabir ME, Saha M, Jeelani S (2007) Effect of ultrasound sonication in carbon nanofibers/polyurethane foam composite. Mater Sci Eng A 459:111–116
Kettler K, Veltman K, Van De Meent D, Van Wezel A, Hendriks AJ (2014) Cellular uptake of nanoparticles as determined by particle properties, experimental conditions, and cell type. Environ Toxicol Chem 33:481–492
Khoee S, Yaghoobian M (2009) An investigation into the role of surfactants in controlling particle size of polymeric nanocapsules containing penicillin-G in double emulsion. Eur J Med Chem 44:2392–2399
Ko JA, Park HJ, Hwang SJ, Park JB, Lee JS (2002) Preparation and characterization of chitosan microparticles intended for controlled drug delivery. Int J Pharm 249:165–174
Larsson M, Huang W-C, Hsiao M-H, Wang Y-J, Nyden M, Chiou S-H, Liu D-M (2013) Biomedical applications and colloidal properties of amphiphilically modified chitosan hybrids. Prog Polym Sci 38:1307–1328
Lee K, Kwon I, Kim Y-H, Jo W, Jeong S (1998) Preparation of chitosan self-aggregates as a gene delivery system. J Controlled Release 51:213–220
Lobo L, Svereika A (2003) Coalescence during emulsification: 2. Role of small molecule surfactants. J Colloid Interface Sci 261:498–507
Mi FL, Shyu SS, Kuan CY, Lee ST, Lu KT, Jang SF (1999a) Chitosan–polyelectrolyte complexation for the preparation of gel beads and controlled release of anticancer drug. I. Effect of phosphorous polyelectrolyte complex and enzymatic hydrolysis of polymer. J Appl Polym Sci 74:1868–1879
Mi FL, Shyu SS, Lee ST, Wong TB (1999b) Kinetic study of chitosan-tripolyphosphate complex reaction and acid-resistive properties of the chitosan-tripolyphosphate gel beads prepared by in-liquid curing method. J Polym Sci Part B Polym Phys 37:1551–1564
Muzzarelli RA (2010) Chitins and chitosans as immunoadjuvants and non-allergenic drug carriers. Marine Drugs 8:292–312
Rocha-Filho P, Camargo M, Ferrari M, Maruno M (2014) Influence of lavander essential oil addition on passion fruit oil nanoemulsions: stability and in vivo study. J Nanomed Nanotechnol 5:198. doi:10.4172/2157-7439.1000198
Salimon J, Salih N, Abdullah BM (2011) Improvement of physicochemical characteristics of monoepoxide linoleic acid ring opening for biolubricant base oil. J Biomed Biotechnol 2011. doi:10.1155/2011/196565
Shu X, Zhu K (2000) A novel approach to prepare tripolyphosphate/chitosan complex beads for controlled release drug delivery. Int J Pharm 201:51–58
Sonvico F, Cagnani A, Rossi A, Motta S, Di Bari M, Cavatorta F, Alonso M, Deriu A, Colombo P (2006) Formation of self-organized nanoparticles by lecithin/chitosan ionic interaction. Int J Pharm 324:67–73
Sun T, Zhang YS, Pang B, Hyun DC, Yang M, Xia Y (2014) Engineered nanoparticles for drug delivery in cancer therapy. Angew Chem Int Ed 53:12320–12364
Tadros T (2013) Encyclopedia of Colloid and Interface Science. Springer. doi:10.1007/978-3-642-20665-8
Takeuchi H, Yamamoto H, Niwa T, Hino T, Kawashima Y (1996) Enteral absorption of insulin in rats from mucoadhesive chitosan-coated liposomes. Pharm Res 13:896–901
Uchegbu IF, Schätzlein AG, Tetley L, Gray AI, Sludden J, Siddique S, Mosha E (1998) Polymeric chitosan-based vesicles for drug delivery. J Pharm Pharmacol 50:453–458
Yoksan R, Jirawutthiwongchai J, Arpo K (2010) Encapsulation of ascorbyl palmitate in chitosan nanoparticles by oil-in-water emulsion and ionic gelation processes. Colloids Surf B 76:292–297
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Motiei, M., Kashanian, S. & Azandaryani, A.H. Effect of Fabrication Parameters on the Physiochemical Properties of Amphiphilic Chitosan Nanoparticles. Iran J Sci Technol Trans Sci 42, 1873–1879 (2018). https://doi.org/10.1007/s40995-017-0152-x
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DOI: https://doi.org/10.1007/s40995-017-0152-x