Abstract
A bone morphogenetic protein-2(BMP-2) derived synthetic oligopeptide, S[PO4]KIPKASSVPTELSAI-STLYLDDD(P24), has shown great potential for facilitating bone regeneration. However, P24 cannot be directly used onto bone defects, while a continuous sustained delivery of P24 may lead to a better formation of bone tissue. Based on this issue, we have developed a sustained delivery system incorporating P24-loaded poly(lactide-co-glycolide) (PLGA) microspheres and nano-hydroxyapatite(n-HA) into the composite hydrogel. The P24-contained compound material was characterized with NMR, FTIR and SEM to demonstrate the formation of compound structure containing P24, PLGA and n-HA. A continuous drug release of P24 was observed for over 60 d that evidently enhanced the efficiency in promoting the proliferation of MC3T3-E1 cells and the secrete of alkaline phosphatase(ALP) in vitro. Moreover, the osteoinduction effect of the hydrogel system with P24 peptide microspheres was demonstrated in vivo and manifested by the result of immunohistochemistry. This novel injectable composite hydrogel is expected to be applied to improving the bone defect treatment in bone tissue engineering.
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Supported by the Research Fund of the Education Department of Jilin Province, China(No. JJKH20190202KJ), the Research Fund of the Development and Reform Commission of Jilin Province, China(No.2019C051-8), the International Cooperation Projects of the Science and Technology Department of Jilin Province, China(No.2018KJT084) and the Health and Family Planning Research Subject of Jilin Province, China(No. 2016Q019).
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CCDC 1816536 contains the supplementary crystallo-graphic data for the compound 1. These data can be obtained free of charge via http://www.ccdc.cam.ac.uk/conts/retrieving.html, or from the Cambridge Crystallographic Data Centre, 12 Union Road, Cambridge CB2 1EZ, UK; fax:(+44) 1223-336-033; or e-mail: deposit@ccdc.cam.ac.uk.
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A Polysaccharide-based Hydrogel and PLGA Microspheres for Sustained P24 Peptide Delivery: An In vitro and In vivo Study Based on Osteogenic Capability
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Cai, Q., Qiao, C., Ning, J. et al. A Polysaccharide-based Hydrogel and PLGA Microspheres for Sustained P24 Peptide Delivery: An In vitro and In vivo Study Based on Osteogenic Capability. Chem. Res. Chin. Univ. 35, 908–915 (2019). https://doi.org/10.1007/s40242-019-9177-3
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DOI: https://doi.org/10.1007/s40242-019-9177-3