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In Vitro Gastrointestinal Lipolysis: Replacement of Human Digestive Lipases by a Combination of Rabbit Gastric and Porcine Pancreatic Extracts

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Food Digestion

Abstract

Various combinations of digestive lipases were tested in vitro under conditions simulating the earlier phases of gastrointestinal lipolysis in the stomach and the duodenum. A solid/liquid test meal was mixed first with either human gastric juice or a solution containing gastric lipase, followed by either the addition of human pancreatic juice and bile or the addition of a solution containing pancreatic lipase, colipase, and bile salts. The rate of lipolysis and the composition of the lipolysis products were assessed as a function of time after lipid extraction and analysis by thin-layer chromatography coupled to flame ionization detection. The lipolytic potential of a crude rabbit gastric extract (RGE) associated with porcine pancreatic extract (PPE) was assessed and compared with the rates of lipolysis of the meal triacylglycerols by human digestive lipases recorded under the same in vitro conditions. RGE combined with PPE appeared to be a good substitute for human gastric and pancreatic lipases. RGE and PPE could therefore be used to simulate the gastrointestinal lipolysis of various foods and emulsions in vitro, as well as that of pharmaceutical lipid formulations.

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Acknowledgments

The authors wish to thank Dr. Jessica Blanc for revising the English manuscript. Our thanks are also due to Dr. Dominique Rainteau and Mrs Lydie Humbert (CHU Saint Antoine Université Pierre et Marie Curie, Faculté de Médecine, Paris) for performing the LC-MS analysis of bovine bile. This work was performed with the financial support of the LISA Carnot Institute and Agence Nationale de la Recherche (Convention n° 07-CARN-009-01).

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Authors and Affiliations

  1. GERME S.A., 27 Boulevard Charles Moretti, 13014, Marseille, France

    Perrine Capolino, Clémence Guérin, Julie Paume, Jacqueline Giallo & Jean-Michel Ballester

  2. Enzymologie Interfaciale et Physiologie de la Lipolyse, UPR 9025, CNRS, Aix-Marseille Université, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France

    Jean-François Cavalier & Frédéric Carrière

Authors
  1. Perrine Capolino
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  2. Clémence Guérin
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  3. Julie Paume
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  4. Jacqueline Giallo
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  6. Jean-François Cavalier
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  7. Frédéric Carrière
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Correspondence to Perrine Capolino, Jacqueline Giallo or Frédéric Carrière.

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Capolino, P., Guérin, C., Paume, J. et al. In Vitro Gastrointestinal Lipolysis: Replacement of Human Digestive Lipases by a Combination of Rabbit Gastric and Porcine Pancreatic Extracts. Food Dig. 2, 43–51 (2011). https://doi.org/10.1007/s13228-011-0014-5

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  • DOI: https://doi.org/10.1007/s13228-011-0014-5

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