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A Genome-Wide Analysis of mRNA Expression in Human Tooth Germ Stem Cells Treated with Pluronic P85

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Abstract

Human tooth germ stem cells (hTGSCs) originate from the neural crest and have a great potential to be used in stem cell therapies. Our group has previously shown that Pluronics interact with stem cells and affect their biological function. Pluronics block copolymer (P85), a potential drug delivery agent in the micelle form, which was shown to improve stem cell expansion. However, it is not known how P85 treatment affects the transcription profile of hTGSCs. In the present study, we found substantial changes in the expression of 252 genes in response to P85 treatment by using Illumina microarray. The gene enrichment was carried out using database for annotation, visualization, and integrated discovery (DAVID) and the results classified in several biologically meaningful clusters. Using bioinformatics tools, we constructed a global regulatory network of P85-modulated genes associated with stem cell differentiation pathways and multi-drug resistance (MDR) processes. In conclusion, our results were compatible with many of the P85-mediated biological processes and may help us to gain a better molecular understanding of P85 biological function.

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Acknowledgments

This study was supported by the Russian Foundation for Basic Research grant 15-04-07527a. The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University and subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities. SVV was supported by RFBR grant for young scientists 16-34-60201. Some of the experiments were conducted using the equipment of Interdisciplinary center for collective use of Kazan Federal University supported by Ministry of Education of Russia (ID RFMEFI59414X0003) and Pharmaceutical Research and Education Center, Kazan (Volga Region) Federal University, Kazan, Russia.

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Authors and Affiliations

  1. Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia

    Atousa Ataei, Valeria V. Solovyeva, Nataliya L. Blatt, Ilnur I. Salafutdinov, Andrey P. Kiyasov & Albert A. Rizvanov

  2. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Mansour Poorebrahim

  3. Department of Genetics and Bioengineering, Yeditepe Universitesi, Istanbul, Turkey

    Fikrettin Şahin & Mehmet E. Yalvaç

  4. Children’s Hospital Columbus, Center for Gene Therapy, Columbus, USA

    Mehmet E. Yalvaç

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  1. Atousa Ataei
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  2. Valeria V. Solovyeva
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  3. Mansour Poorebrahim
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  4. Nataliya L. Blatt
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  5. Ilnur I. Salafutdinov
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  8. Mehmet E. Yalvaç
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  9. Albert A. Rizvanov
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Correspondence to Albert A. Rizvanov.

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Ataei, A., Solovyeva, V.V., Poorebrahim, M. et al. A Genome-Wide Analysis of mRNA Expression in Human Tooth Germ Stem Cells Treated with Pluronic P85. BioNanoSci. 6, 392–402 (2016). https://doi.org/10.1007/s12668-016-0254-5

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