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Pulse Duration and Efficiency of Soft Cellular Tissue Disintegration by Pulsed Electric Fields

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Abstract

The effect of pulse duration on efficiency of disintegration of apple tissue by pulsed electric fields (PEF) was studied. The samples (26-mm diameter, 10-mm height) were treated by PEF at electric field strength E between 100 and 400 V/cm, pulse duration t i of 10, 100, 1,000 μs, inter-pulse duration Δt of 100 μs and different number of pulses n. Both the degree and the time evolution of tissue damage were quantified by electrical conductivity disintegration index Z and characteristic damage time τ, respectively. The samples exposed to the same PEF treatment time nt i showed noticeably higher disintegration efficiency for larger pulse duration. The synergism of PEF and thermal treatment with temperature T (20–50 °C) was demonstrated. The Arrhenius dependence of τ(T) for PEF treatment at E = 100 V/cm gave the decreasing activation energy W as a function of t i, (Q ≈ 164 kJ/mol at t i = 10 μs, Q ≈ 109 kJ/mol at t i = 100 μs and Q ≈ 66 kJ/mol at t i = 1,000 μs). Textural relaxation data supported the higher damage efficiency for longer pulse duration.

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Abbreviations

PEF:

pulsed electric fields

C :

specific capacitance of cell membrane (F/m2)

D :

sample diameter (mm)

E :

electric field strength (V/cm)

F :

force (N)

h :

sample height (mm)

h m :

membrane thickness (nm)

n :

number of pulses

N :

number of trains

r 2 :

coefficient of determination

R :

radius of a spherical cell (m)

R g :

=8.314 J K−1 mol−1, the universal gas constant

t c :

membrane charging time (s)

t I :

pulse duration (s)

t PEF :

total time of electrical treatment (s)

Δt :

inter-pulse duration (s)

Δt t :

inter-train pause (s)

T :

temperature (°C)

Q :

activation energy (kJ/mol)

u m :

transmembrane potential (V)

W :

electrical energy input (KJ/kg)

Z :

electrical conductivity disintegration index

d:

maximally damaged

e:

extracellular medium

m:

membrane

max:

maximum

u:

untreated

τ :

characteristic damage time (s)

τ ∞ :

limiting characteristic damage time (s)

θ :

angle between the external field E and the radius-vector r

σ :

electrical conductivity (S/m)

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Acknowledgments

The authors appreciate financial support from the “Pole Regional Genie des Procedes” (Picardie, France). Authors also thank Dr. N.S. Pivovarova for her help with preparation of the manuscript.

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Authors and Affiliations

  1. Centro Regionale di Competenza Produzioni Agroalimentari, University of Salerno, Via Ponte don Melillo, 84084, Fisciano (Salerno), Italy

    Francesca De Vito & Giovanna Ferrari

  2. Department of Chemical and Food Engineering, University of Salerno, Via Ponte don Melillo, 84084, Fisciano (Salerno), Italy

    Giovanna Ferrari

  3. Departement de Génie des Procédés Industriels, Université de Technologie de Compiègne, Centre de Recherche de Royallieu, B.P. 20529-60205, Compiègne Cedex, France

    Nikolai I. Lebovka, Nikolai V. Shynkaryk & Eugéne Vorobiev

  4. Institute of Biocolloidal Chemistry, 42, blvr. Vernadskogo, Kiev, 03142, Ukraine

    Nikolai I. Lebovka & Nikolai V. Shynkaryk

  5. Département Génie Chimique Laboratoire de Génie des Procédés Industriels, Université de Technologie de Compiègne, UMR CNRS 6067, BP 20529 60205, Compiègne, France

    Eugéne Vorobiev

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  1. Francesca De Vito
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  4. Nikolai V. Shynkaryk
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  5. Eugéne Vorobiev
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Correspondence to Eugéne Vorobiev.

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De Vito, F., Ferrari, G., I. Lebovka, N. et al. Pulse Duration and Efficiency of Soft Cellular Tissue Disintegration by Pulsed Electric Fields. Food Bioprocess Technol 1, 307–313 (2008). https://doi.org/10.1007/s11947-007-0017-y

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  • DOI: https://doi.org/10.1007/s11947-007-0017-y

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