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Preparation and functional characterization of tumor-targeted folic acid-chitosan conjugated nanoparticles loaded with mitoxantrone

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Abstract

Folic acid conjugated chitosan was prepared by cross-linking reaction with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and then used as a template to prepare folic acid-chitosan (FA–CS) conjugated nanoparticles and load mitoxantrone nanoparticles (FA-CSNP/MTX). Drug dissolution testing, CCK-8 method, and confocal microscopy were used to detect their controlled-release capability in different situations and the specific uptake by HONE1 cells. The experimental results show that the nanoparticles have uniform size distribution of 48–58 nm. The highest encapsulation rate of the particles on mitoxantrone hydrochloride (MTX) is (77.5±1.9)%, and the drug loading efficiency is (18.4±0.4)%. The sustained release effect, cell growth inhibition activity and targeting effect of the FA-CS/MTX nanoparticles are good in artificial gastric fluid and intestinal fluid. It is demonstrated that the FA-CSNP system is a potentially useful system for the targeted delivery of anticancer drug MTX.

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Authors and Affiliations

  1. Dongguan Research Center, Guangdong Medical College, Dongguan, 523808, China

    Wei Wang  (王炜), Xing-yan Liu  (刘星言) & Tao Li  (李涛)

  2. College of Biology, Hunan University, Changsha, 410082, China

    Chun-yi Tong  (童春义) & Bin Liu  (刘斌)

  3. Department of Ophthalmology and Eye Research Center, The 2nd Xiangya Hospital, Central South University, Changsha, 410011, China

    Wei Xiong  (熊炜)

Authors
  1. Wei Wang  (王炜)
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  2. Chun-yi Tong  (童春义)
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  3. Xing-yan Liu  (刘星言)
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  4. Tao Li  (李涛)
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  5. Bin Liu  (刘斌)
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  6. Wei Xiong  (熊炜)
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Correspondence to Wei Xiong  (熊炜).

Additional information

Foundation item: Projects(31201074, 81371013) supported by the National Natural Science Foundation of China; Project(2011105102016) supported by the Key Program of Medical Health of Dongguan City, Guangdong Province, China; Project(2011108102026) supported by Dongguan Universities Program, China

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Wang, W., Tong, Cy., Liu, Xy. et al. Preparation and functional characterization of tumor-targeted folic acid-chitosan conjugated nanoparticles loaded with mitoxantrone. J. Cent. South Univ. 22, 3311–3317 (2015). https://doi.org/10.1007/s11771-015-2871-5

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  • DOI: https://doi.org/10.1007/s11771-015-2871-5

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