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Gold Nanoparticle Based FRET for DNA Detection

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Abstract

The nanoscience revolution that sprouted throughout the 1990s is having great impact in current and future DNA detection technology around the world. In this review, we report our recent progress on gold nanoparticle based fluorescence resonance energy transfer assay to monitor DNA hybridization as well as the cleavage of DNA by nucleases. We tried to discuss a reasonable account of the science and the important fundamental work carried out in this area. We also report the development of a compact, highly specific, inexpensive and user-friendly optical fiber laser-induced fluorescence sensor based on fluorescence quenching by nanoparticles to detect single-strand DNA hybridization at femtomolar level.

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Acknowledgement

Dr. Ray thanks NSF-CRIFMU grant # 0443547, NSF-PREM grant # DMR-0611539, ARO grant # W911NF-06-1-0512 and NIH-SCORE grant # S06GM 008047 for their generous funding. William Hardy thanks NSF/LSMAMP grant # HRD-0115807 for his scholarship during matriculation. We also thank reviewers whose valuable suggestion improved the quality of the manuscript.

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  1. Department of Chemistry, Jackson State University, 1400 JR Lynch Street, Jackson, MS, 39218, USA

    Paresh Chandra Ray, Gopala Krishna Darbha, Anandhi Ray, Joshua Walker & William Hardy

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  1. Paresh Chandra Ray
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  2. Gopala Krishna Darbha
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  3. Anandhi Ray
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Correspondence to Paresh Chandra Ray.

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Ray, P.C., Darbha, G.K., Ray, A. et al. Gold Nanoparticle Based FRET for DNA Detection. Plasmonics 2, 173–183 (2007). https://doi.org/10.1007/s11468-007-9036-9

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