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Near-Infrared Fluorescence Enhancement Using Silver Island Films

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Abstract

Near-infrared (near-IR) excitation produces little background signal from biological molecules, making near-IR fluorescence technology highly useful in proteomic and genomic applications. To increase the emissions of near-IR fluorophores, we examined the use of metal-enhanced fluorescence on these longer wavelength dyes. IRDye®700- and IRDye®800-labeled DNA oligonucleotides and proteins were spotted onto silver island film (SIF)-coated glass slides, and analyzed using a LI-COR Odyssey® IR imaging system. We observed more than 18-fold enhancement of the IRDye®700 and 15-fold enhancement of the IRDye®800-labeled DNA oligonucleotides when spotted on SIF-coated surfaces compared with uncoated surfaces. We also demonstrated that the enhanced emissions produced on the SIF-coated slides remained linear over several orders of magnitude, that the emissions remained reproducible across a slide surface, and that the SIF-coated slide remained effective at enhancing emissions after 9 months of storage. Our results indicate that SIF-coated glass slides are effective at enhancing near-IR fluorescence and could be developed into an effective tool to aid in molecular biological applications.

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Acknowledgments

This work was supported by the NIH National Center for Research Resources, Small Business Innovation Research grant RR021785. We also thank the Center for Fluorescence Spectroscopy, which is supported by the National Center for Research Resources grant RR08119, for their assistance.

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  1. LI-COR Biosciences, Inc., 4308 Progressive Avenue, P.O. Box 4000, Lincoln, NE, 68504, USA

    Jon P. Anderson, Mark Griffiths & Vince R. Boveia

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  1. Jon P. Anderson
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  2. Mark Griffiths
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  3. Vince R. Boveia
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Correspondence to Jon P. Anderson.

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Anderson, J.P., Griffiths, M. & Boveia, V.R. Near-Infrared Fluorescence Enhancement Using Silver Island Films. Plasmonics 1, 103–110 (2006). https://doi.org/10.1007/s11468-006-9018-3

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