Skip to main content
SpringerLink
Log in

Study on the specific interaction between angiogenin and aptamer by atomic force microscopy (AFM)

  • Articles
  • Analytical Chemistry
  • Published:
Chinese Science Bulletin

Abstract

The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other elements in a series of control experiments. The results have shown that there is specific interaction force between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7±11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer, which provided basis for further understanding the inhibition of the aptamer to angiogenic activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fett J W, Strydom D J, Lobb R R, et al. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells. Biochem, 1985, 24(20): 5480–5486

    Article  CAS  Google Scholar 

  2. Shapiro R, Strydom D J, Olson K A, et al. Isolation of angiogenin from normal human plasma. Biochem, 1987, 26(16): 5141–5146

    Article  CAS  Google Scholar 

  3. Bai D Y, Ma H D. The role of angiopoietin in angiogenesis. Acta Academiae Medicinae CPAPF (in Chinese), 2005, 14(4): 323–325

    CAS  Google Scholar 

  4. Ahmad S A, Liu W, Jung Y D, et al. The effects of angiopoietin-1 and -2 on tumor growth and angiogenesis in human colon cancer. Cancer Res, 2001, 61(4): 1255–1259

    PubMed  CAS  Google Scholar 

  5. Eggert A, Ikegaki N, Kwiatkowski J, et al. High-level expression of angiogenic factors is associated with advanced tumor stage in human neuroblastomas. Clin Cancer Res, 2000, 6(5): 1900–1908

    PubMed  CAS  Google Scholar 

  6. Papapetropoulos A, Fulton D, Mahboubi K, et al. Angiopoietin-1 inhibits endothelial cell apoptosis via the Akt/ survivin pathway. J Biol Chem, 2000, 275(13): 9102–9105

    Article  PubMed  CAS  Google Scholar 

  7. Wurmbach J H, Hammerer P, Sevine S, et al. The expression of angiopoietins and their receptor Tie-2 in human prostate carcinoma. Anticancer Res, 2000, 20: 5217–5220

    PubMed  CAS  Google Scholar 

  8. Gao D L, Guo K M, Seng J J, et al. Expression of angtopoietin-2 and Tie-2 in gastric carcinoma tissues. Journal of Medical Forum (in Chinese), 2005, 26(20): 40–41

    Google Scholar 

  9. Nobile V, Russo N, Hu G F, et al. Inhibition of human angiogenin by DNA aptamers: nuclear colocalization of an angiogenin-inhibitor complex. Biochem. 1998, 37(19): 6857–6863

    Article  CAS  Google Scholar 

  10. Li Y X, He F C. The methods for studies of interaction between DNA and proteins. Chemistry of Life (in Chinese), 2003, 23(4): 306–308

    CAS  Google Scholar 

  11. Yang L, Wang K M, Tan W H, et al. Atomic force microscopy study of different effects of natural and semi-synthetic beta-lactam on the cell envelope of Escherichia coli. Anal Chem. 2006, 78: 7341–7345

    Article  PubMed  CAS  Google Scholar 

  12. Yamashina S, Shigeno M. Application of atomic force microscopy to ultrastructural and histochemical studied of fixed and embedded cells. J Electron Microsc, 1995, 44(6): 462–466

    CAS  Google Scholar 

  13. Mingorance J, Tadros M, Vicente M, et al. Visualization of single Escherichia coli FtsZ filament dynamics with atomic force microscopy. J Biol Chem, 2005, 280(21): 20909–20914

    Article  PubMed  CAS  Google Scholar 

  14. Wang Z G, Wan L J, Zhou C Q, et al. Study of â-amyloid adsorption and aggregation on graphite by STM and AFM. Chin Sci Bull, 2003, 48(5): 437–440

    Article  CAS  Google Scholar 

  15. Qin F, Jiang Y X, Ma X Y, et al. A study of specific interaction of the transcription factor and the DNA element by atomic force microscopy. Chin Sci Bull, 2004, 49(13): 1376–1380

    Article  CAS  Google Scholar 

  16. Allen S, Chen X Y, Davies J, et al. Detection of antigen-antibody binding envents with the atomic force microscope. Biochem, 1997, 36(24): 7457–7463

    Article  CAS  Google Scholar 

  17. Yuan C, Chen A, Kolb P, et al. Energy landscape of streptavidin-biotin complexes measured by atomic force microscopy. Biochem, 2000, 39(33): 10219–10223

    Article  CAS  Google Scholar 

  18. Ros R, Schwesinger F, Anselmetti D, et al. Antigen binding forces of individually addressed single-chain Fv antibody molecules. Proc Natl Acad Sci USA, 1998, 95(13): 7402–7405

    Article  PubMed  CAS  Google Scholar 

  19. Lo Y S, Huefner N D, Chan W S, et al. Specific Interactions between biotin and avidin studied by atomic force microscopy using the Poisson statistical analysis method. Langmuir, 1999, 15(4): 1373–1382

    Article  CAS  Google Scholar 

  20. Wong J, Chilkoti A, Moy V T. Direct force measurements of the streptavidin-biotin interaction. Biomolecular Engineering, 1999, 16(1–4): 45–55

    Article  PubMed  CAS  Google Scholar 

  21. Jiang Y X, Zhu C F, Ling L S, et al. Specific Aptamer-Protein Interaction Studied by Atomic Force Microscopy. Anal Chem, 2003, 75(9): 2112–2116

    Article  PubMed  CAS  Google Scholar 

  22. Basnar B, Elnathan R, Willner I. Following aptamer-thrombin binding by force measurements. Anal Chem, 2006, 78(11): 3638–3642

    Article  PubMed  CAS  Google Scholar 

  23. Jin Y, Wang K M, Tan W H, et al. Monitoring molecular beacon/DNA interactions using atomic force microscopy. Anal Chem, 2004, 76(19): 5721–5725

    Article  PubMed  CAS  Google Scholar 

  24. Lo Y S, Simons J, Beebe T P. Loading-rate dependence of individual ligand-receptor bond-rupture forces studied by atomic force microscopy. Langmuir, 2001, 17(12): 3741–3748

    Article  CAS  Google Scholar 

  25. Williams J M, Han T, Beebe T P J. Determination of single-bond forces from contact force variances in atomic force microscopy. Langmuir, 1996, 12(5): 1291–1295

    Article  CAS  Google Scholar 

  26. Stevens F, Lo Y S, Harris J M, et al. Computer modeling of atomic force microscopy force measurements: comparisons of Poisson, Histogram, and Continuum methods. Langmuir, 1999, 15(1): 207–213

    Article  CAS  Google Scholar 

  27. Wenzler L A, Moyes G L, Olson L G, et al. Single-molecule bond-rupture force analysis of interactions between AFM tips and substrates modified with organosilanes. Anal Chem, 1997, 69(14): 2855–2861

    Article  CAS  Google Scholar 

  28. Tian S X, Cui X Y. The investigation of human angiogenin. Chemistry of Life (in Chinese), 1996, 16(3): 25–26

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Life Science and Biological Technology, Engineering Center for Biomedicine, College of Material Science and Technology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Changsha, 410082, China

    He XiaoXiao, Jin Rong, Yang Liu, Wang KeMin, Li Wei, Tan WeiHong & Li HuiMin

Authors
  1. He XiaoXiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jin Rong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wang KeMin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Li Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tan WeiHong
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Li HuiMin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wang KeMin.

Additional information

Supported by the National Key Basic Research Program (Grant No. 2002CB513100-10), Key Technologies Research and Development Program (Grant No. 2003BA310A16), Key Project Foundation of China Education Ministry (Grant No. 107084), Program for New Century Excellent Talents in University (Grant No. NCET-06-0697), National Natural Science Foundation of China (Nos. 90606003 and 20405005) and Outstanding Youth Foundation of Hunan Province (Grant No. 06JJ10004)

About this article

Cite this article

He, X., Jin, R., Yang, L. et al. Study on the specific interaction between angiogenin and aptamer by atomic force microscopy (AFM). Chin. Sci. Bull. 53, 198–203 (2008). https://doi.org/10.1007/s11434-007-0494-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-007-0494-4

Keywords

Search

Navigation