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Association of melanin content with conidiogenesis in Bipolaris Sorokiniana of barley (Hordeum vulgare L.)

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Abstract

Sixty-seven isolates of Bipolaris sorokiniana of barley, belonging to three groups (black, white and mixed) were studied to find an association of melanin with the spore production of the fungus. Conidiogenesis in black, white and mixed subpopulation of B. sorokiniana was positively correlated with melanin content/g of mycelium. Primary hyphae of black and mixed subpopulation differentiated into secondary hyphal structures which subsequently produced conidiophores and conidia. Primary hyphae could not differentiate into secondary hyphae and subsequently conidiophores and conidia in white subpopulation. A melanin containing mutant developed from white subpopulation regained its ability to differentiate into secondary hyphae, conidiophores and conidia. Results showed that melanization of mycelia B. sorokiniana mycelia is an important factor for conidia production.

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References

  • Bell AA, Wheeler MH (1986) Biosynthesis and functions of fungal melanins. Annu Rev Phytopathol 24:411–451

    Article  CAS  Google Scholar 

  • Butler MJ, Day AW (1998) Destruction of fungal melanins by ligninases of Phanerochaete chrysosporium and other white rot fungi. Int J Plant Sci 159:989–995

    CAS  Google Scholar 

  • Butler MJ, Day AW, Henson JM, Money NP (2001) Pathogenic properties of fungal melanins. Mycologia 93:1–8

    Article  CAS  Google Scholar 

  • Carzaniga R, Fiocco D, Bowyer P, O’Connell RJ (2002) Localization of melanin in conidia of Alternaria alternata using phage display antibodies. Mol Plant–Microbe Interact 15(3):216–224

    Article  CAS  Google Scholar 

  • Chand R, Singh HV, Joshi AK, Duveiller E (2002) Physiological and morphological aspects of Bipolaris sorokiniana on wheat straw. Plant Pathol J 18(6):328–332

    Google Scholar 

  • Chand R, Pandey SP, Singh HV, Kumar S, Joshi AK (2003) Variability and its probable cause in the natural populations of spot blotch pathogen Bipolaris sorokiniana of wheat (T. aestivum L.) in India. J Plant Dis Protection 110:27–35

    Google Scholar 

  • Chand R, Sen D, Prasad KD, Singh AK, Bashyal BM, Prasad LD, Joshi AK (2008) Screening for disease resistance in barley cultivars against Bipolaris sorokiniana using callus culture method. Indian J Exp Biology 36:249–253

    Google Scholar 

  • Eliahu N, Igbaria A, Rose MS, Horwitz BA, Lev S (2007) Melanin biosynthesis in the maize pathogen Cochliobolus heterostrophus depends on two mitogen-activated protein kinases, Chk1 and Mps1, and the transcription factor Cmr1. Eukaryot Cell 6(3):421–442

    Article  CAS  Google Scholar 

  • Elliott ML (1995) Effect of melanin biosynthesis inhibiting compounds on Gaeumannomyces species. Mycologia 87:370–374

    Article  CAS  Google Scholar 

  • Eyal Z, Scharen AL, Prescott JM, van Ginkel M (1987) The septoria disease of wheat: concepts and methods of disease management. CIMMYT, Mexico, DF

  • Frederick BA, Caesar-TonThat TC, Wheeler M, Sheehan KB, Edens WA, Henson JM (1999) Isolation and characterization of Gaeumannomyces graminis melanin mutants. Mycol Res 103:99–110

    Article  CAS  Google Scholar 

  • Gadd GM (1982) Effects of media composition and light on colony differentiation and melanin synthesis in Microdochium bolleyi. Trans Br Mycolo Soc 78:115–122

    Article  CAS  Google Scholar 

  • Henson JM, Butler MJ, Day AW (1999) The dark side of the mycelium: melanins of phytopathogenic fungi. Ann Rev Phytopathol 37:447–471

    Article  CAS  Google Scholar 

  • Jaiswal SK, Sweta S, Prasad LC, Sharma S, Kumar S, Prasad R, Pandey SP, Chand R, Joshi AK (2007) Identification of molecular marker and aggressiveness for different groups of Bipolaris sorokiniana isolates causing spot blotch disease in wheat (Triticum aestivum L.). Curr Microbiol 55:135–141

    Article  CAS  Google Scholar 

  • Kawamura C, Moriwaki J, Kimura N, Fujita Y, Fuji S, Hirano T, Koizumi S, Tsuge T (1997) The melanin biosynthesis genes of Alternaria alternata can restore pathogenicity of the melanin deficient mutant of Magnaporthe grisea. Mol Plant–Microbe Interact 10:445–453

    Article  Google Scholar 

  • Kawamura C, Tsujimoto T, Tsuge T (1999) Targeted disruption of a melanin biosynthesis gene affects conidial development and UV tolerance in the Japanese pear pathotype of Alternaria alternata. Mol Plant–Microbe Interact 12:59–63

    Article  CAS  Google Scholar 

  • Kendrick B, Chang MG (1971) Karyology of conidiogenesis in some hyphomycetes. In: Kendrick B (ed) Taxonomy of fungi imperfecti. University of Toronto Press, Toronto, pp 278–291

    Google Scholar 

  • Kumar D, Chand R, Prasad LC, Joshi AK (2007) A new technique for monoconidial culture of the most aggressive isolate in a given population of Bipolaris sorokiniana, cause of foliar spot blotch in wheat and barley. World J Microbial Biotechnol 23:1647–1651

    Article  CAS  Google Scholar 

  • Leach J, Lang BR, Yoder DC (1982) Methods for selection of mutants and in vivo culture of Cochliobolus heterostrophus. J Microbiol 128:1719–1729

    Article  Google Scholar 

  • Mishra AP (1981) Variability, physiologic specialization and genetics of pathogenicity in graminicolus Helminthosporium affecting cereal crops. Ind Phytopathol 34:1–22

    Google Scholar 

  • Nelson RR (1960) Evolution of sexuality and pathogenicity I. Interspecific crosses in the genus Helminthosporium. Phytopathol 50:375–377

    Google Scholar 

  • Pandey SP, Sharma S, Chand R, Shahi P, Joshi AK (2008) Clonal variability and its relevance in generation of new pathotypes in the spot blotch pathogen, B. sorokiniana. Curr Microbiol 56:33–41

    Article  CAS  Google Scholar 

  • Rizner TL, Wheeler MH (2003) Melanin biosynthesis in the fungus Curvularia lunata (teleomorph: Cochliobolus lunatus). Can J Microbiol 49:110–119

    Article  CAS  Google Scholar 

  • Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico D.F, pp 37–38

    Google Scholar 

  • Romero-Martinez R, Wheeler M, Guerrero-Plata A, Rico G, Torres-Guerrero H (2000) Biosynthesis and function of melanin in Sporothrix schenckii. Infect Immunol 68:3696–3703

    Google Scholar 

  • Saari EE, Prescott JM (1975) A scale for appraising the foliar intensity of wheat disease. Plant Dis Rep 59:377–380

    Google Scholar 

  • Saghai Maroof MA, Soliman KM, Jorgensen RA, Allarde RW (1984) Ribosomal RNA-spacer-length polymorphism in barley: Mendalian inheritance chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Article  CAS  Google Scholar 

  • Shimizu K, Tanaka C, Tsuda M (1997) Cloning of Brn 1, a reductase gene involved in melanin biosynthesis in Cochliobolus heterostrophus. J Gen Appl Microbiol 43:145–150

    Article  CAS  Google Scholar 

  • Sussman AS (1968) Longevity and survivability of fungi. In: Ainsworth GC, Sussman AS (eds) The fungi, vol III. Academic Press, New York, pp 447–486

    Google Scholar 

  • Tanabe K, Park P, Tsuge T, Kohmoto K, Nishimura S (1995) Characterization of the mutants of Alternaria alternata Japanese pear pathotype deficient in melanin production and their pathogenicity. Ann Phytopathol Soc Jpn 61:27–33

    CAS  Google Scholar 

  • Tinline RD (1961) Cochliobolus sativus V. Heterokayosis and parasexualtiy. Can J Bot 40:425–437

    Article  Google Scholar 

  • Zadock JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421

    Article  Google Scholar 

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Acknowledgments

Authors are thankful to University Grant Commission for the financial assistance.

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Authors and Affiliations

  1. Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    B. M. Bashyal, Ramesh Chand & Chanda Kushwaha

  2. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Devyani Sen, L. C. Prasad & A. K. Joshi

Authors
  1. B. M. Bashyal
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  2. Ramesh Chand
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  3. Chanda Kushwaha
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  4. Devyani Sen
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  5. L. C. Prasad
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  6. A. K. Joshi
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Correspondence to Ramesh Chand.

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Bashyal, B.M., Chand, R., Kushwaha, C. et al. Association of melanin content with conidiogenesis in Bipolaris Sorokiniana of barley (Hordeum vulgare L.). World J Microbiol Biotechnol 26, 309–316 (2010). https://doi.org/10.1007/s11274-009-0177-1

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  • DOI: https://doi.org/10.1007/s11274-009-0177-1

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