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Role of polyamines in plant tissue culture: An overview

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Abstract

Since the era of plant tissue culture bloomed, we have started approaching from a biotechnological perspective to overcome the massive challenges like inducing embryogenesis and organogenesis, initiating rooting, increasing the number of plantlets, establishing a callus from various organs of plants and also enhancing the metabolite content which was a mind-boggling thought once upon a time. Use of various elicitors, altering the media components, the strength of media, pH, precursor feeding etc. have all contributed tremendously in the in-vitro techniques used for culturing rare, endemic and medicinal plants for the commercial purposes. Owing to the demand for the plant products and drugs, the search for the other superior novel methods to increase its quantity and quality has not been stopped. Thus, one such method is the use of chemical compounds with many amino groups which serves as an additional source of nitrogen in the media and these organic compounds are called polyamines. Polyamines are known to play a wide role in plant physiological processes helping them in differentiation, inducing totipotency, increasing cell division and also in molecular signaling. Polyamines have a versatile application in this field ranging from establishing a callus to the elicitation of secondary metabolites. Thus, polyamines can be considered as a boon to the plant tissue culture field. In this review article, we have mainly focused on the in-depth applications of major polyamines like putrescine, spermidine and spermine in the field of plant tissue culture.

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Abbreviations

Pas:

Polyamines

Put:

Putrescine

Spd:

Spermidine

Spm:

Spermine

IBA:

Indole-3-butyric acid

NAA:

Naphthaleneacetic acid

BA:

Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

DFMA:

Difluoromethylarginine

DFMO:

Difluoromethylornithine

MGBG:

Methyl-glyoxyl-bis guanylhydrazone

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Acknowledgements

We would like to acknowledge Miss. Smita Dhantal, PhD Scholar, Dept. of English and Cultural Studies, CHRIST (Deemed To Be University) for proof reading the article and commenting on the grammatical and linguistic aspects.

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  1. Department of Life Sciences, CHRIST (Deemed To Be University), Hosur Road, Bengaluru, 560 029, India

    B. Rakesh, W. N. Sudheer & Praveen Nagella

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Rakesh, B., Sudheer, W.N. & Nagella, P. Role of polyamines in plant tissue culture: An overview. Plant Cell Tiss Organ Cult 145, 487–506 (2021). https://doi.org/10.1007/s11240-021-02029-y

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