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Gold nanoparticles chemisorbed by a terphenyldithiol self-assembled monolayer for fabrication of a protein biosensor

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Abstract

In this study, improved detection of bovine serum albumin (BSA) was achieved by use of a fabricated surface plasmon resonance (SPR) biosensor. Terphenyldithiol (TPDT) was self-assembled on a gold substrate, then gold nanoparticles (Au-NPs) were chemisorbed on to the TPDT monolayer by strong bonding with the terminal thiol groups of the TPDT. The new sensor obtained was tested for immobilization of protein. The SPR results revealed much better detection of BSA by Au-NPs chemisorbed on the TPDT self-assembled monolayer (SAM) than by the bare SAM on the gold substrate.

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Acknowledgments

N.A.F. Al-Rawashdeh gratefully acknowledges financial support by Jordan University of Science and Technology, Jordan. W. Azzam gratefully acknowledges financial support by Tafila Technical University, Jordan. The authors gratefully acknowledge I. Shamyleh for his help with SPR experiments.

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Authors and Affiliations

  1. Department of Chemistry, United Arab Emirates University, P.O. Box 17551, Al Ain, UAE

    Nathir A. F. Al-Rawashdeh

  2. Department of Applied Chemical Sciences, Jordan University of Science & Technology, P.O. Box 3030, Irbid, 22110, Jordan

    Nathir A. F. Al-Rawashdeh

  3. Department of Chemistry, Tafila Technical University, P.O. Box 179, Tafila, 66110, Jordan

    W. Azzam

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  1. Nathir A. F. Al-Rawashdeh
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  2. W. Azzam
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Correspondence to Nathir A. F. Al-Rawashdeh.

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Al-Rawashdeh, N.A.F., Azzam, W. Gold nanoparticles chemisorbed by a terphenyldithiol self-assembled monolayer for fabrication of a protein biosensor. Res Chem Intermed 37, 759–770 (2011). https://doi.org/10.1007/s11164-011-0309-8

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  • DOI: https://doi.org/10.1007/s11164-011-0309-8

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