Abstract
Aims
The aim of this study was to investigate the mechanisms by which nitrogen (N) and phosphorous (P) additions affect plant C:N:P stoichiometry via changes in AMF communities in a Chinese fir plantation.
Methods
Experimental plots in a 10-year-old Chinese fir plantation were treated with N addition (LN, 30 kg•ha−1•yr−1, and HN, 60 kg•ha−1•yr−1) and P addition (LP, 20 mg•kg−1, and HP, 40 mg•kg−1) for 2 years starting from 2017. Soil properties, leaf C:N:P stoichiometry, and changes in AMF community composition and diversity were measured.
Results
N or P addition increased soil N availability, decreased P availability, and increased N:P in the leaves and soil. Changes in soil pH induced by N and P additions affected the colonization rate and spore density of AMF. AMF colonization rate was increased by N or P addition, and AMF diversity was increased by LN addition. N and P additions altered AMF species composition, and the dominant genus of the AMF in the soil was Glomus in all treatments. The interaction of N and P treatments had no significant effect on AMF diversity. AMF colonization rate and diversity, rather than its composition, affected leaf C:N:P stoichiometry.
Conclusion
N and P additions affected colonization rate and diversity of AMF though changes in soil pH, which drove leaf C:N:P stoichiometry, and potentially affected forest productivity via the fungi-soil-plant system.
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Acknowledgements
The study was funded by the National Key Research and Development Program of China (Grant No.2016YFD0600201) and the National Natural Science Foundation of China (NSFC) (Grant No. 31971623). We also thank Ting Wang and Zhenge Shi for their help during the experiment.
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Xinzhang Song designed the experiments. Yikang Shen and Quan Li performed the experiments and analyzed the data. Meihua Liu, Yikang Shen and Xinzhang Song wrote the draft. Xinzhang Song had the overall responsibility for the study.
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Liu, M., Shen, Y., Li, Q. et al. Arbuscular mycorrhizal fungal colonization and soil pH induced by nitrogen and phosphorus additions affects leaf C:N:P stoichiometry in Chinese fir (Cunninghamia lanceolata) forests. Plant Soil 461, 421–440 (2021). https://doi.org/10.1007/s11104-021-04831-1
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DOI: https://doi.org/10.1007/s11104-021-04831-1