Abstract
Gold nanorod (GNR) has great potential in the field of cancer therapy because of its photophysical property in converting near-infrared (NIR) laser light into heat. Fabrication of GNRs by seed-mediated growth method with the aid of cetyltrimethylammonium bromide (CTAB) is a popular approach. However, due to high cytotoxicity of CTAB, it is necessary to modify the surface of CTAB-passivated GNRs for cell-related studies. In this study, thiolated chitosan was synthesized and harnessed to replace CTAB originally used to stabilize GNRs. The average size and morphological shape of CTAB-passivated GNRs (66.0 nm) and thiolated chitosan-modified GNRs (CGNRs) (84.9 nm) were determined by dynamic light scattering and transmission electron microscopy. X-ray photoelectron spectroscopy was used to confirm the existence of Au–S binding energy at 162.4 eV. Cytotoxicity study revealed that CGNRs were much biocompatible than CTAB-stabilized GNRs. Our results showed that CGNRs functionalized with folic acid (FA) could be internalized by human colon HT-29 cancer cells via folate-mediated endocytosis. From the viability of CGNR-laden HT-29 cells irradiated with 808-nm NIR laser light, we demonstrated that CGNR is a potential photothermal nano-absorber for the ablation of malignant cells under NIR laser exposure.
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Alkilany AM, Nagaria PK, Hexel CR, Shaw TJ, Murphy CJ, Wyatt MD (2009) Cellular uptake and cytotoxicity of gold nanorods: molecular origin of cytotoxicity and surface effects. Small 5:701–708
Au L, Zheng D, Zhou F, Li ZY, Li X, Xia Y (2008) A quantitative study on the photothermal effect of immune gold nanocages targeted to breast cancer cells. ACS Nano 2:1645–1652
Bernkop-Schnurch A, Hornof M, Guggi D (2004) Thiolated chitosans. Eur J Pharm Biopharm 57:9–17
Bhattarai SR, Kc RB, Kim SY, Sharma M, Khil MS, Hwang PH, Chung GH, Kim HY (2008) N-hexanoyl chitosan stabilized magnetic nanoparticles: implication for cellular labeling and magnetic resonance imaging. J Nanobiotechnol 6:1
Cathell MD, Szewczyk JC, Bui FA, Weber CA, Wolever JD, Kang J, Schauer CL (2008) Structurally colored thiol chitosan thin films as a platform for aqueous heavy metal ion detection. Biomacromolecules 9:289–295
Chang KLB, Tai MC, Cheng FH (2001) Kinetics and products of the degradation of chitosan by hydrogen peroxide. J Agric Food Chem 49:4845–4851
Chen J, Glaus C, Laforest R, Zhang Q, Yang M, Gidding M, Welch MJ, Xia Y (2010) Gold nanocages as photothermal transducers for cancer treatment. Small 6:811–817
Cho JH, Kim SH, Park KD, Jung MC, Yang WI, Han SW, Noh JY, Lee JW (2004) Chondrogenic differentiation of human mesenchymal stem cells using a thermosensitive poly(N-isopropylacrylamide) and water-soluble chitosan copolymer. Biomaterials 25:5743–5751
Connor EE, Mwamuka J, Cole A, Murphy CJ, Wyatt MD (2005) Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small 1:325–327
Dai Q, Coutts J, Zou J, Huo Q (2008) Surface modification of gold nanorods through a place exchange reaction inside an ionic exchange resin. Chem Commun 25:2858–2860
Ellman GL (1958) A colorimetric method for determining low concentrations of mercaptans. Arch Biochem Biophys 74:443–450
Felt O, Buri P, Gurny R (1998) Chitosan: a unique polysaccharide for drug delivery. Drug Dev Ind Pharm 24:979–993
Foss CA, Hornyak GL, Stockert JA, Martin CR (1994) Template-synthesized nanoscopic gold particles: optical spectra and the effects of particle size and shape. J Phys Chem 98:2963–2971
Hirsch LR, Stafford RJ, Bankson JA, Sershen SR, Price RE, Hazle JD, Halas NJ, West JL (2003) Nanoshell-mediated near infrared thermal therapy of tumors under magnetic resonance guidance. Proc Natl Acad Sci USA 100:13549–13554
Huang X, El-Sayed IH, Qian W, El-Sayed MA (2006) Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods. J Am Chem Soc 128:2115–2120
Huang X, Jain PK, El-Sayed IH, El-Sayed MA (2007) Gold nanoparticles: interesting optical properties and recent applications in cancer diagnostics and therapy. Nanomedicine 2:81–693
Huff TB, Hansen MN, Zhao Y, Cheng JX, Wei A (2007) Controlling the cellular uptake of gold nanorods. Langmuir 23:1596–1599
Jana NR, Gearheart L, Murphy CJ (2001) Wet chemical synthesis of high aspect ratio cylindrical gold nanorods. J Phys Chem B 105:4065–4067
Kam NW, O’Connell M, Wisdom JA, Dai H (2005) Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destruction. Proc Natl Acad Sci USA 102:11600–11605
Liao H, Hafner LH (2005) Gold nanorod bioconjugates. Chem Mater 17:4636–4641
Link S, El-Sayed MA (2000) Shape and size dependence of radiative, nonradiative, and photothermal properties of gold nanocrystals. Int Rev Phys Chem 19:409–433
Liu XF, Guan YL, Yang DZ, Li Z, Yao KD (2001) Antibacterial action of chitosan and carboxymethylated chitosan. J Appl Polym Sci 79:1324–1335
Loo C, Lowery A, Halas NJ, West JL, Drezek R (2005) Immunotargeted nanoshells for integrated cancer imaging and therapy. Nano Lett 5:709–711
Niidome T, Yamagata M, Okamoto Y, Akiyama Y, Takahashi H, Kawano T, Katayama Y, Niidome Y (2006) PEG-modified gold nanorods with a stealth character for in vivo applications. J Control Release 114:343–347
Nikoobakht B, El-Sayed MA (2001) Evidence for bilayer assembly of cationic surfactants on the surface of gold nanorods. Langmuir 17:6368–6374
Orendorff CJ, Murphy CJ (2006) Quantitation of metal content in the silver-assisted growth of gold nanorods. J Phys Chem B 110:3990–3994
Satake K, Okuyama T, Ohashi M, Shinoda T (1960) The spectrophotometric determination of amine, amino acid and peptide with 2,4,6-trinitrobenzene 1-sulfonic acid. J Biochem 47:654–660
Takahashi H, Niidome Y, Niidome T, Kaneko K, Kawasaki H, Yamada S (2006) Modification of gold nanorods using phosphatedylcholine to reduce cytotoxicity. Langmuir 22:2–5
Tong L, Zhao Y, Huff TB, Hansen MN, Wei A, Cheng JX (2007) Gold nanorods mediate tumor cell death by compromising membrane integrity. Adv Mater 19:3136–3141
Wang CH, Huang YJ, Chang CW, Hsu WM, Peng CA (2009) In vitro photothermal destruction of neuroblastoma cells using carbon nanotubes conjugated with GD2 monoclonal antibody. Nanotechnology 20:315101–315107
Wang CH, Chiou SH, Chou CP, Chen YC, Huang YJ, Peng CA (2010) Photothermolysis of glioblastoma stem-like cells targeted by carbon nanotubes conjugated with CD133 monoclonal antibody. Nanomedicine. doi:10.1016/j.nano.2010.06.010
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This study was funded by Taiwan National Science Council grant (NSC96-2628-E-002-013-MY3) and Michigan Tech Fund of Michigan Technological University.
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Chung-Hao Wang and Chia-Wei Chang have contributed equally to this work.
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Wang, CH., Chang, CW. & Peng, CA. Gold nanorod stabilized by thiolated chitosan as photothermal absorber for cancer cell treatment. J Nanopart Res 13, 2749–2758 (2011). https://doi.org/10.1007/s11051-010-0162-5
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DOI: https://doi.org/10.1007/s11051-010-0162-5