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A novel magnetic fluid based on starch-coated magnetite nanoparticles functionalized with homing peptide

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Abstract

Preparation and characterization in vitro and in vivo of a novel magnetic fluid based on starch-coated magnetite nanoparticles functionalized with homing peptide is reported in this paper. Precursory magnetic fluids stabilized with starch were prepared, in a polymeric starch matrix, by controlled chemical coprecipitation of magnetite phase from aqueous solutions. The average hydrodynamic diameter of starch-coated iron oxide nanoparticles (SIONs) was 46 nm. As a homing peptide, A54 is the most effective peptide specific to the human hepatocellular carcinoma cell line BEL-7402. Final magnetic fluids were obtained through chemical coupling of homing peptide labeled with 5-carboxyl-fluorescein (FAM-A54) and SIONs. Magnetic measurements showed the saturation magnetization value of SIONs amounted to 45 emu/g and the FAM-A54-coupled SIONs showed a good magnetic response in magnetic field. The results of experiments in vitro and in vivo showed that SIONs were endowed with specific affinity to corresponding tumor cells after coupling with FAM-A54 and the FAM-A54-coupled SIONs could be accumulated in the tumor tissue with more efficiency than individual magnetic targeting or biomolecular targeting. This novel magnetic fluid with dual function has great potential applications in diagnostics and therapeutics of human tumor such as drug targeting, magnetic hyperthermia, and magnetic resonance imaging.

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Acknowledgment

This research project is supported by Shanghai Nanotechnology Promotion Center (0352nm113, 0852nm03200).

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Authors and Affiliations

  1. Department of Physics, Center of Functional Nanomaterials and Devices, East China Normal University, North Zhongshan Rd. 3663, Shanghai, 200062, People’s Republic of China

    Ji-Sen Jiang, Zhi-Feng Gan & Yong Yang

  2. School of Life Science, East China Normal University, Shanghai, 200062, People’s Republic of China

    Bing Du, Min Qian & Ping Zhang

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  1. Ji-Sen Jiang
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  2. Zhi-Feng Gan
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  3. Yong Yang
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  4. Bing Du
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  5. Min Qian
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  6. Ping Zhang
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Correspondence to Ji-Sen Jiang.

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Jiang, JS., Gan, ZF., Yang, Y. et al. A novel magnetic fluid based on starch-coated magnetite nanoparticles functionalized with homing peptide. J Nanopart Res 11, 1321–1330 (2009). https://doi.org/10.1007/s11051-008-9534-5

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  • DOI: https://doi.org/10.1007/s11051-008-9534-5

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