Skip to main content
SpringerLink
Log in

Physical and chemical characterization insulin-loaded chitosan-TPP nanoparticles

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

The purpose of this study was to develop and characterize insulin nanoparticles systems using chitosan. Insulin-loaded nanoparticles were prepared by ionic gelation of chitosan with tripolyphosphate anions (TPP). The interactions between insulin and chitosan were evaluated by differential scanning calorimetry (DSC), thermogravimetry/derivative thermogravimetry (TG/DTG), and Fourier-transform infrared (FTIR) spectroscopy. Besides, particle size distribution, polydispersity index (PDI), zeta potential, and association efficiency (AE%) of the nanoparticles were evaluated. In general, inert nanoparticles and insulin-loaded nanoparticles showed an average size of 260.56 nm (PDI 0.502) and 312.80 nm (PDI 0.481), respectively. Both nanoparticles showed positive charge, but after insulin incorporation the zeta potential was reduced, evidencing its incorporation. Nanoparticles obtained also showed AE% around 70%, measured by high-performance liquid chromatography (HPLC). The results of FTIR, DSC, and TG/DTG corroborated the data presented suggesting that insulin was successfully encapsulated. However, drug incorporation seems to be related not only to electrostatic interactions, but also to physical process and/or adsorption phenomena.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Janes KA, Fresneau MP, Marazuela A, Fabra A, Alonso MJ. Chitosan nanoparticles as delivery systems for doxorubicin. J Control Release. 2001;73:255–67.

    Article  CAS  Google Scholar 

  2. Calvo P, Remunan-Lopez C, Vila-Jato JL, Alonso MJ. Chitosan and chitosan/ethylene oxide–propylene oxide block copolymer nanoparticles as novel carriers for proteins and vaccines. Pharm Res. 1997;14:1431–6.

    Article  CAS  Google Scholar 

  3. Ko JA, Park HJ, Hwang SJ, Park JB, Lee JS. Preparation and characterization of chitosan microparticles intended for controlled drug delivery. Int J Pharm. 2002;249:165–74.

    Article  CAS  Google Scholar 

  4. Kumari K, Raina KK, Kundu PP. DSC studies on the curing kinetics of chitosan–alanine using glutaraldehyde as crosslinker. J Therm Anal Calorim. 2009;98:469–76.

    Article  CAS  Google Scholar 

  5. Ajun W, Yan S, Li G, Huili L. Preparation of aspirin and probucol in combination loaded chitosan nanoparticles and in vitro release study. Carbohydr Polym. 2009;75:566–74.

    Article  Google Scholar 

  6. Barichello JM, Morishita M, Takayama K, Nagai T. Absorption of insulin from Pluronic F-127 gels following subcutaneous administration in rats. Int J Pharm. 1999;184:189–98.

    Article  CAS  Google Scholar 

  7. Mesiha MS, Sidhom MB, Fasipe B. Oral and subcutaneous absorption of insulin poly(isobutylcyanoacrylate) nanoparticles. Int J Pharm. 2005;288:289–93.

    Article  CAS  Google Scholar 

  8. Ramachandran R, Paul W, Sharma CP. Synthesis and characterization of PEGylated calcium phosphate nanoparticles for oral insulin delivery. J Biomed Mater Res B Appl Biomater. 2009;88:41–8.

    Google Scholar 

  9. Fernández-Urrusuno R, Calvo P, Remuñán-López C, Vila-Jato JL, Alonso MJ. Enhancement of nasal absorption of insulin using chitosan nanoparticles. Pharm Res. 1999;16:1576–81.

    Article  Google Scholar 

  10. Pan Y, Li YJ, Zhao HY, Zheng JM, Xu H, Wei G, Hao JS, Cui FD. Bioadhesive polysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo. Int J Pharm. 2002;249:139–47.

    Article  CAS  Google Scholar 

  11. Ma Z, Lim TM, Lim LY. Pharmacological activity of peroral chitosan-insulin nanoparticles in diabetic rats. Int J Pharm. 2005;293:271–80.

    Article  CAS  Google Scholar 

  12. Sarmento B, Ribeiro A, Veiga F, Sampaio P, Neufeld R, Ferreira D. Alginate/chitosan nanoparticles are effective for oral insulin delivery. Pharm Res. 2007;24:2198–206.

    Article  CAS  Google Scholar 

  13. Sarmento B, Ferreira D, Veiga F, Ribeiro A. Characterization of insulin-loaded alginate nanoparticles produced by ionotropic pre-gelation through DSC and FTIR studies. Carbohydr Polym. 2006;66:1–7.

    Article  CAS  Google Scholar 

  14. Ma Z, Yeoh HH, Lim LY. Formulation pH modulates the interaction of insulin with chitosan nanoparticles. J Pharm Sci. 2002;91:1396–404.

    Article  CAS  Google Scholar 

  15. Bayat A, Dorkoosh FA, Dehpour AR, Moezi L, Larijani B, Junginger HE, Rafiee-Tehrani M. Nanoparticles of quaternized chitosan derivatives as carrier for colon delivery of insulin: ex vivo and in vivo studies. Int J Pharm. 2008;356:59–60.

    Article  Google Scholar 

  16. Gan Q, Wang T, Cochrane C, McCarron P. Modulation of surface charge, particle size and morphological properties of chitosan-TPP nanoparticles intended for gene delivery. Colloids Surf B Biointerfaces. 2005;44:65–73.

    Article  CAS  Google Scholar 

  17. Santos JE, Soares JP, Dockal ER, Campana Filho SP, Cavalheiro ETG. Caracterização de quitosanas comerciais de diferentes origens. Polím Ciên Tecnol. 2002;13:242–9.

    Google Scholar 

  18. Peniche C, Elvira C, Roman JS. Interpolymer complexes of chitosan and poly-methacrylic derivatives of salicylic acid: preparation, characterization and modification by thermal treatment. Polymer. 1998;34:6549–54.

    Article  Google Scholar 

  19. Boonsongrit Y, Mueller BW, Mitrevej A. Characterization of drug–chitosan interaction by 1H NMR, FTIR and isothermal titration calorimetry. Eur J Pharm Biopharm. 2008;69:388–95.

    Article  CAS  Google Scholar 

  20. Sarmento B, Ribeiro A, Veiga F, Ferreira D. Development and characterization of new insulin containing polysaccharide nanoparticles. Colloids Surf B Biointerfaces. 2006;53:193–202.

    Article  CAS  Google Scholar 

  21. Bhumkar DR, Pokharkar VB. Studies on effect of pH on cross-linking of chitosan with sodium tripolyphosphate: a technical note. AAPS PharmSciTech. 2006;7:1–6. http://www.aapspharmscitech.org.

  22. Xu Y, Du Y. Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles. Int J Pharm. 2003;250:215–26.

    Article  CAS  Google Scholar 

  23. Pikal MJ, Rigsbee DR. The stability of insulin in crystalline and amorphous solids: observation of greater stability for the amorphous form. Pharm Res. 1997;14:1379–87.

    Article  CAS  Google Scholar 

  24. Lai MC, Topp EM. Solid-state chemical stability of proteins and peptides. J Pharm Sci. 1999;88:489–99.

    Article  CAS  Google Scholar 

  25. Boonsongrit Y, Mitrevej A, Mueller BW. Chitosan drug binding ionic interaction. Eur J Pharm Biopharm. 2006;62:267–74.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/Brazil), Fundação de Amparo à Pesquisa do Estado de Sergipe (FAPITEC/SE) and (CAPES/Brazil) for the financial support, and the Secretary of State for Health in Aracaju city for the insulin donation.

Author information

Authors and Affiliations

  1. Departamento de Fisiologia, Universidade Federal de Sergipe, Av. Marechal Rondon, s/n, Cidade Universitária, São Cristóvão, Sergipe, CEP 49100-000, Brazil

    J. R. Azevedo, R. H. Sizilio, M. B. Brito, A. M. B. Costa, M. R. Serafini, A. A. S. Araújo, M. R. V. Santos, A. A. M. Lira & R. S. Nunes

Authors
  1. J. R. Azevedo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. H. Sizilio
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. B. Brito
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. M. B. Costa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. R. Serafini
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. A. S. Araújo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. R. V. Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. A. M. Lira
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. R. S. Nunes
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. S. Nunes.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Azevedo, J.R., Sizilio, R.H., Brito, M.B. et al. Physical and chemical characterization insulin-loaded chitosan-TPP nanoparticles. J Therm Anal Calorim 106, 685–689 (2011). https://doi.org/10.1007/s10973-011-1429-5

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10973-011-1429-5

Keywords

Search

Navigation