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Preparation and characterization of magnetic nanoparticles embedded in hydrogels for protein purification and metal extraction

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Abstract

The present work involves the development of hydrogel magnetic nanocomposites for protein purification and heavy metal extraction applications. The magnetic nanoparticles (MNPs) were prepared in situ in poly(acrylamide)-gum acacia (PAM-GA) hydrogels. The formation of magnetic nanoparticles in the hydrogel networks was confirmed by Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). Scanning electron (SEM) microscopy studies revealed the formation of MNPs throughout the hydrogel networks. The average size of MNPs formed in the hydrogel networks was 3–5 nm as determined by transmission electron microscopy (TEM). The thermal properties of the hydrogel magnetic nanocomposites were evaluated by dynamic scanning calorimetry (DSC) and thermogravimetric (TG) analysis. The magnetic properties of the developed hydrogel magnetic nanocomposites were determined by a vibrating sample magnetometer (VSM). The swelling properties of the hydrogel and the hydrogel magnetic nanocomposites were studied in detail. The hydrogel magnetic nanocomposites are utilized for the removal of toxic metal ions such as Co(II), Ni(II), and Cu(II) and for protein purification. The results confirm that the hydrogel magnetic nanocomposites exhibit superior extraction properties to hydrogels.

Poly(acrylamide)-gum acacia (PAM-GA) magnetic nanocomposite hydrogels were synthesized to evaluate their applicability for protein purification and metal extraction

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Acknowledgement

KMR and NNR thank the University Grants Commission (UGC), Government of India, New Delhi for the partial financial support (UGC Sanction Letter No.F.37-339/2009 (SR) dated 05-01-2010).

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  1. Murali Mohan Yallapu

    Present address: Cancer Biology Research Center, Sanford Research/USD, Sioux Falls, SD, 57105, USA

Authors and Affiliations

  1. Synthetic Polymer Laboratory, Department of Polymer Science and Technology, Sri Krishnadevaraya University, Anantapur, 515 055, AP, India

    Narayana Reddy Nagireddy, Murali Mohan Yallapu, Varaprasad Kokkarachedu, Ravindra Sakey, Vimala Kanikireddy & Mohana Raju Konduru

  2. Physical & Materials Chemistry Division, National Chemical Laboratory, Pune, India

    Joy Pattayil Alias

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  1. Narayana Reddy Nagireddy
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Correspondence to Mohana Raju Konduru.

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Nagireddy, N.R., Yallapu, M.M., Kokkarachedu, V. et al. Preparation and characterization of magnetic nanoparticles embedded in hydrogels for protein purification and metal extraction. J Polym Res 18, 2285–2294 (2011). https://doi.org/10.1007/s10965-011-9642-2

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  • DOI: https://doi.org/10.1007/s10965-011-9642-2

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