Abstract
We have developed a naturally-derived crosslinker named malic acid derivative (MAD). In the present study, we prepared alkali-treated collagen (AlCol) gels with different crosslinkers including MAD and commercially available crosslinkers such as 1-ethyl-3-(3(′-dimethylaminopropyl) carbodiimide (EDC) and glutaraldehyde (GA). There are named as AlCol-MAD, AlCol-EDC, and AlCol-GA. We then compared their physicochemical properties. The residual amino groups in AlCol-MAD were not detected at MAD concentrations higher than 30 mM. On the other hand, the residual amino groups in AlCol-EDC and AlCol-GA were detected at crosslinker concentrations of 30 mM. The swelling ratios of AlCol-MAD, AlCol-EDC, and AlCol-GA decreased with increasing crosslinker concentration. Enzymatic degradation rate of AlCol-GA was slower than that of AlCol-MAD and AlCol-EDC. The cytotoxicity of MAD was clearly lower than that of EDC and GA. The number of adhered L929 on AlCol-MAD was higher than on AlCol-EDC and AlCol-GA after incubation for 1 day. After the culture for 3 and 7 days, excellent growth of L929 was observed on AlCol-MAD. These results suggested that MAD was excellent crosslinker for the reactivity with amino groups and cytocompatibility. Therefore, the resulting AlCol-MAD has potential for various biomedical applications like tissue engineering scaffolds and carrier for drug delivery systems.
Similar content being viewed by others
References
C. J. DOILLON and F. H. SILVE, Biomaterials 7 (1986) 3
M. E. NIMNI, in: “Encyclopedic Handbook of Biomaterials and Engineering. Part A: Materials”, edited by D. L. WISE (Marcel Dekker, New York, 1995) p. 1229
S. T. LI, in “The Biomedical Engineering Handbook”, edited by J. D. BRONZINO (CRC Press in Corporation with IEEE Press, Boca Raton, FL, 1995) p. 627
M. J. B. WISSINK, R. BEERNINK, J. S. PIEPER, A. A. POOT, G. H. M. ENGBERS, T. BEUGELING, W. G. AKEN and J. FEIJEN, Biomaterials 22 (2001) 151
R. GHISELLI, A. GIACOMETTI, L. GOFFI, O. CIRIONI, F. MOCCHEGIANI, F. ORLANDO, A. M. PAGGI, E. PETRELLI, G. SCALISE and V. SABA, J. Surg. Res. 99 (2001) 316
H. W. SUNG, Y. CHANG, C. T. CHIU, C. N. CHEN and H. C. LIANG, Biomaterials 20 (1999) 1759
S. M. WELLS and M. S. SACKS, Biomaterials 23 (2002) 2389
A. SANO, M. MAEDA, S. NAGAHARA, T. OCHIYA, K. HONMA, H. ITOH, T. MIYATA and K. FUJIOKA, Adv. Drug. Deliv. Rev. 55 (2003) 1651
A. JAYAKRISHNAN and S. R. JAMEELA, Biomaterials 17 (1996) 471
S. B. LEE, Y. H. KIM, M. S. CHONG and Y. M. LEE, Biomaterials 25 (2004) 2309
J. HART, D. SILCOCK, S. GUNNIGLE, B. CULLEN, N. D. LIGHT and P. W. WATT, Int. J. Biochem. Cell. Biol. 34 (2002) 1557
G. CHEN, T. SATO, M. SAKANE, H. OHGUSHI, T. USHIDA, J. TANAKA and T. TATEISHI, Mater. Sci. Eng. C 24 (2004) 373
G. CHEN, T. SATO, M. SAKANE, H. OHGUSHI, T. USHIDA, J. TANAKA and T. TATEISHI, Mater. Sci. Eng. C 24 (2004) 861
Q. LU, K. GANESAN, D. T. SIMIONESCU and N. R. VYAVAHARE, Biomaterials 25 (2004) 5227
A. BIGI, G. COJAZZI, S. PANZAVOLTA, K. RUBINI and N. ROVERI, Biomaterials 22 (2001) 763
J. E. GOUGH, C. A. SCOTCHFORD and S. DOWNES, J. Biomed. Mater. Res. 61 (2002) 121
S. N. JAN PARK, J. C. PARK, H. O. KIM, M. J. SONG and H. SUH, Biomaterials 23 (2002) 1205
K. BILLAR, J. MURRAY, D. LAUDE, G. ABRAHAM and N. BACHRACH, J. Biomed. Mater. Res. 56 (2001) 101
P. B. VAN WACHEM, R. ZEEMAN, P. J. DIJKSTRA, J. FEIJEN, M. HENDRINKS, P. T. CAHALAN and M. J. A. VAN LUYN, J. Biomed. Mater. Res. 47, 270 (1999)
F. DE LUSTRO, R. A. CONDELL, M. A. NGUYEN and J. M. MCPHERSON, J. Biomed. Mater. Res. 20 (1986) 109
A. COOKE, R. F. OLIVER and M. EDWARD, Br. J. Exp. Pathol. 64 (1983) 172
G. GOISSIS, E. MARCANTONIO JR., R. A. MARCANTONIO, R. C. LIA, D. C. CANCIAN and W. M. DE CARVALHO, Biomaterials 20 (1999) 27
J. M. MCPHERSON, S. SAWAMURA and R. ARMSTRON, J. Biomed. Mater. Res. 20 (1986) 93
L. H. H. HUANG-LEE, D. T. CHEUNG and M. E. NIMNI, J. Biomed. Mater. Res. 24 (1990) 1185
R. J. LEVY, F. J. SCHOEN, F. S. SHERMAN, J. NICHOLS, M. A. HAWEY and S. A. LUND, Am. J. Pathol. 122 (1986) 71
R. J. SCHOEN and R. J. LEVY, Am. J. Pathol. 123 (1986) 134
J. E. LEE, J. C. PARK, Y. S. HWANG, J. K. KIM, J. G. KIM and H. SUH, Yonsei Med. J. 42 (2001) 172
K. S. WEADOCK, E. J. MILLER, E. L. KEUFFEL and M. G. DUNN, J. Biomed. Mater. Res. 32 (1996) 221
S. D. GORHAM, N. D. LIGHT, A. M. DIAMOND, M. J. WILLINS, A. J. BAILEY, T. J. WESS and N. J. LESLIE, Int. J. Biol. Macromol. 14 (1992) 129
T. TAGUCHI, H. SAITO, M. IWASASHI, M. SAKANE, S. KAKINOKI and J. TANAKA, J. Nanosci. Nanotechnol. 7 (2007) 742
T. TAGUCHI, H. SAITO, Y. UCHIDA, M. SAKANE, H. KOBAYASHI, K. KATAOKA and J. TANAKA, Mater. Sci. Eng. C 24 (2004) 775
H. SAITO, T. TAGUCHI, H. AOKI, K. KATAOKA, S. MURABAYASHI, Y. MITAMURA, J. TANAKA and T. TATEISHI, Acta Biomater. 3 (2007) 89
H. SAITO, T. TAGUCHI, H. KOBAYASHI, K. KATAOKA, J. TANAKA, S. MURABAYASHI and Y. MITAMURA, Mater. Sci. Eng. C 24 (2004) 781
T. TAGUCHI, H. SAITO, M. IWASASHI, M. SAKANE, S. KAKINOKI, N. OCHIAI and T. TATEISHI, Key. Eng. Mater. 330–332 (2007) 1339
W. A. BUBNIS and C. M. OFNER III, Anal. Biochem. 207 (1992) 129
S. N. PARK, J. C. PARK, H. O. KIM, M. J. SONG and H. SUH, Biomaterials 23 (2002) 1205
J. RANKE, K. MOLTER, F. STOCK, U. BOTTIN-WEBER, J. POCZOBUTT, J. HOFFMANN, B. ONDRUSCHKA, J. FILSER and B. JASTORFF, Ecotox. Environ. Safe. 60 (2005) 350
Z. ZHANG, R. ROY, F. J. DUGRE, D. TESSIER and L. H. DAO, J. Biomed. Mater. Res. 57, 63 (2001)
K. E. PFITZNER and J. G. MOFFATT, J. Am. Chem. Soc. 87 (1965) 5661
L. H. H. OLDE DAMINK, P. J. DIJKSTRA, M. J. A. VAN LUYN, P. B. VAN WACHEM, P. NIEUWENHUIS and J. FEIJEN, Biomaterials 17 (1996) 765
K. ULUBAYRAM, E. AKSU, S. I. D. GURHAN, K. SERBETCI and N. HASIRCI, J. Biomater. Sci. Polymer Edn. 13 (2002) 1203
K. ULUBAYRAM, E. AKSU, S. I. D. GURHAN, K. SERBETCI and N. HASIRCI, J. Biomater. Sci. Polymer Edn. 13 (2002) 1203
L. H. H. OLDE DAMINK, P. J. DIJKSTRA, M. J. A. VAN LUYN, P. B. VAN WACHEM, P. NIEUWENHUIS and J. FEIJEN, J. Mater. Sci. Mater. Med. 6 (1995) 460
A. MONTEMBAULT, C. VITON and A. DOMARD, Biomaterials 26 (2005) 1633
V. CHARULATHA and A. RAJARAM, Biomaterials 24 (2003) 759
K. ULUBAYRAM, E. AKSU, S. I. D. GURHAN, K. SERBETCI and N. HASIRCI, J. Biomater. Sci. Polymer Edn. 13 (2002) 1203
G. A. DIGENIS, T. B. GOLD and V. P. SHAH, J. Pharm. Sci. 83 (1994) 915
A. J. KUIJPERS, G. H. M. ENGBERS, J. FEIJEN, S. C. DE SMEDT, T. K. L. MEYVIS, J. DEMEESTER, J. KRIJGSVELD, S. A. J. ZAAT and J. DANKERT, Macromolecules 32 (1999) 3325
J. HARDIN-YOUNG, R. M. CARR, G. J. DOWNING and K. D. CONDON, Biotechnol. Bioeng. 49 (1996) 675
J. H. LEE, A. K. GO, S. H. OH, K. E. LEE and S. H. YUK, Biomaterials 26 (2005) 671
C. R. NUTTELMAN, S. M. HENRY and K. ANSETH, Biomaterials 23 (2002) 3617
Acknowledgments
This work was financially supported in part by Innovative Technology Research Grant from Japan Science and Technology Agency (JST), Japan, and the Coordination Found for Prompting Science and Technology for Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan (Development of artificial organs utilizing with Nanotechnology and materials science) and Industrial Technology Research Grant Program in ‘04 from New Energy and Industrial Technology Development Organization (NEDO) of Japan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Saito, H., Murabayashi, S., Mitamura, Y. et al. Characterization of alkali-treated collagen gels prepared by different crosslinkers. J Mater Sci: Mater Med 19, 1297–1305 (2008). https://doi.org/10.1007/s10856-007-3239-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10856-007-3239-7