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Tissue spinal cord response in rats after implants of polypyrrole and polyethylene glycol obtained by plasma

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Abstract

Most of the biomaterials used nowadays for the reconstruction of the spinal cord (SC) tissue after an injury, tested in animals, have obtained modest results. This work presents a study about the compatibility of two novel, non-biodegradable, semi-conductive materials, obtained by plasma polymerization: iodine-doped pyrrole (PPy/I) and pyrrole-polyethylene glycol (PPy/PEG). Both polymers, separately, were implanted in the SC tissue of rats after a transection. Prior to implantation, the elemental composition and the physico-chemical properties of polymers were studied by electron scanning microscopy, IR Spectroscopy and thermogravimetric analysis. We used adult female Long Evans rats, subjected to SC transection. Animals were randomized to be allocated in one of the treatment groups and were killed four weeks after the lesion for histology study. Results showed that both implants were integrated to the SC tissue, as inflammatory and gliotic responses, similar to those observed in the control group, and rejection of the implant, were not evident. Moreover, the immediate effect of PPy/I or PPy/PEG in the injured SC prevented secondary tissue destruction, as compared to non-implanted control animals. In conclusion, implants of semi-conductive polymers were well-tolerated and integrated favorably to SC tissue after transection

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Acknowledgments

We thank to Dr. Jorge Guevara, Dr. Gabriel Guizar-Sahagún and Tec. Noemi Gelista Herrera from the National Institute of Neurology and Neurosurgery of Mexico, for their valuable assistance in the analyzing and capture of images. This work was supported by CONACyT grant No. 47467.

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Authors and Affiliations

  1. Departamento de Física, Universidad Autónoma Metropolitana Iztapalapa, Mexico, Mexico

    Roberto Olayo, Juan Morales, Ana Laura Alvarez, Rodrigo Mondragon & Axayacatl Morales

  2. Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez S.S.A, Av. Insurgentes Sur 3877, Mexico, D.F, 14269, Mexico

    Camilo Ríos, Mireya Alcaraz-Zubeldia & Araceli Diaz-Ruiz

  3. Unidad de Investigación Médica en Enfermedades Neurológicas, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Mexico, Mexico

    Hermelinda Salgado-Ceballos

  4. Centro de Investigación del proyecto CAMINA A.C., Mexico, Mexico

    Hermelinda Salgado-Ceballos

  5. Departamento de Síntesis y Caracterización, Instituto Nacional de Investigaciones Nucleares, Mexico, Mexico

    Guillermo Jesus Cruz & Maria Guadalupe Olayo

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  1. Roberto Olayo
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  2. Camilo Ríos
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Correspondence to Araceli Diaz-Ruiz.

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Olayo, R., Ríos, C., Salgado-Ceballos, H. et al. Tissue spinal cord response in rats after implants of polypyrrole and polyethylene glycol obtained by plasma. J Mater Sci: Mater Med 19, 817–826 (2008). https://doi.org/10.1007/s10856-007-3080-z

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  • DOI: https://doi.org/10.1007/s10856-007-3080-z

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