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Effect of bisphosphonates on the stimulation of macrophages by alumina ceramic particles: A comparison with ultra-high-molecular-weight polyethylene

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Abstract

Wear particle-induced osteolysis and loosening is a critical process that limits the longevity of total hip arthroplasty. Despite their potential value in the management of aseptic loosening, little is known about the cellular response to bisphosphonates (BPs) in the presence of particulate debris. In the present study, we compared the effect of pamidronate and clodronate, two structurally different bisphosphonates, on the induction of TNF-α release by alumina ceramic (Al2O3) and ultra-high-molecular-weight-polyethylene (UHMWPE) particles. We also looked, by Trypan blue exclusion, at the viability of J774 mouse macrophages incubated with Al2O3 and UHMWPE particles in combination with pamidronate or clodronate. Results showed that pamidronate and clodronate can inhibit UHMWPE particle-induced TNF-α release while they had no effect on Al2O3-stimulated TNF-α release. The co-incubation of pamidronate or clodronate and Al2O3 had no effect on the induction by Al2O3 of poly(ADP-ribose)polymerase (PARP) proteolysis and DNA fragmentation. On the other hand, UHMWPE particles had no effect on these apoptotic markers. However, the co-incubation of pamidronate or clodronate with UHMWPE particles led to the appearance of these markers of apoptosis. Al2O3 and UHMWPE particles had no effect on macrophage cell death or the number of macrophages at the end of experiments. Co-incubation of UHMWPE particles with pamidronate and clodronate led to a significant increase in cell death. Interestingly, the number of macrophages co-incubated with particles and pamidronate or clodronate significantly decreased. In conclusion, our results suggest that the effect of BPs on particle-stimulated macrophages is, at least in part, particle composition dependent.

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References

  1. D. W. HOWIE, B. MANTHEY, S. HAY and B. VERNON-ROBERTS, Clin. Orthop. Rel. Res. 292 (1993) 352.

    Google Scholar 

  2. S. GOODMAN, J. S. WANG, D. REGULA and P. ASPENBERG, Acta Orthop. Scand. 65 (1994) 157.

    Article  CAS  Google Scholar 

  3. J. ANTONIOU, O. HUK, D. ZUKOR, D. EYRE and M. ALINI, J. Orthop. Res. 18 (2000) 64.

    Article  CAS  Google Scholar 

  4. A. S. SHANBHAG, C.T. HASSELMAN and H.E. RUBASH, Clin. Orthop. Rel. Res. 44 (1997) 33.

    Article  Google Scholar 

  5. C. J. ROSEN and C. R. KESSENICH, Drugs. 51 (1996) 537.

    Article  CAS  Google Scholar 

  6. F. R. SINGER, Current Opin. Orthop. 7 (1996) 26.

    Article  Google Scholar 

  7. S. M. HOROWITZ and J. B. GONZALES, J. Orthop. Res. 15 (1997) 50.

    Article  CAS  Google Scholar 

  8. C. P. RADER, B. BAUMANN, T STERNER, O. ROLF, C. HENDRICH, N. SCHUTZE and E. JACOBS, Biomediz Technik. 44 (1999) 135.

    Article  CAS  Google Scholar 

  9. O. L. HUK, D. J. ZUKOR, J. ANTONIOU and A. PETIT, J. Orthop. Res. 21 (2003) 81.

    Article  CAS  Google Scholar 

  10. P. CAMPBELL, S. MA, B. YEOM, H MCKELLOP, T. P. SCHMALZRIED and H. C. AMSTUTZ, J. Biomed. Mater. Res. 29 (1995) 131.

    Article  Google Scholar 

  11. J. M. DORLOT, P. CHRISTEL and A MEUNIER, J. Biomed. Mater. Res. 23 (A3 Suppl) (1989) 299.

    Article  CAS  Google Scholar 

  12. S. LEROUGE, O. HUK, L’H. YAHIA and L. SEDEL, J. Biomed. Mater. Res. 32 (1996) 627.

    Article  CAS  Google Scholar 

  13. A. PETIT, I. CATELAS, D. J. ZUKOR and O. L. HUK, J. Orthop. Res. 20 (2002) 9.

    Article  CAS  Google Scholar 

  14. R. G. RUSSELL, M. J. ROGERS, J. C. FRITH, S. P. LUCKMAN, F. P. COXON, HL BENFORD, P. I. CROUCHER, C. SHIPMAN and H. A. FLEISCH, J. Bone Miner. Res. 14 (Suppl 2) (1999) 53.

    Article  CAS  Google Scholar 

  15. S. M. HOROWITZ, S. B. DOTY, J. M. LANE and A. H. BURSTEIN, J. Bone Joint Surg. [Am]. 75-A (1993) 802.

    Google Scholar 

  16. S. P. LUCKMAN, F. P. COXON, F. H. EBITINO, RGG RUSSELL and M. J. ROGERS, J. Bone Miner. Res. 13 (1998) 1668.

    Article  CAS  Google Scholar 

  17. M. J. ROGERS, K. M. CHILTON, F. P. COXON, J. LAWRY, M. O. SMITH, S. SURI and G. G. RUSSELL, J. Bone Miner. Res. 11 (1996) 1482.

    CAS  Google Scholar 

  18. S. M. ALGAN, M. PURDON and S. M. HOROWITZ, J. Orthop. Res. 14 (1996) 30.

    Article  CAS  Google Scholar 

  19. I. CATELAS, A. PETIT, R. MARCHAND, D. J. ZUKOR, L’H. YAHIA and O. L. HUK, J. Bone Joint Surg. [Br]. 81-B (1999) 516.

    Article  Google Scholar 

  20. A. PRABHU, C. E. SHELBURNE and D. F. GIBBONS, J. Biomed. Mater. Res. 42 (1998) 655.

    Article  CAS  Google Scholar 

  21. K. D. MERKEL, J. M. ERDMANN, K. P. MCHUGH, Y. ABU-AMER, F. P. ROSS and S. L. TEITELBAUM, Am. J. Pathol. 154 (1999) 203.

    CAS  Google Scholar 

  22. A. U. DANIELS, F. H. BARNES, S. J. CHARKEBOIS and R. A. SMITH, J. Biomed. Mater. Res. 49 (2000) 469.

    Article  CAS  Google Scholar 

  23. R. A. BROOKS, J. A. WIMHURST and N. RUSHTON, J. Bone Joint Surg. [Br]. 84-B (2002) 295.

    Article  Google Scholar 

  24. T. S. TENGKU-MUHAMMAD, T. R. HUGHES, A. CRYER and D. J. RAMJI, Cytokine. 7 (1999) 463.

    Article  Google Scholar 

  25. B. GAO and M. F. TSAN, Biochem. Biophys. Res. Commun. 317 (2004) 1149.

    Article  CAS  Google Scholar 

  26. R. L. THERIAULT and G. N. HORTOBAGYI, Semin. Oncol. 28 (2001) 284.

    Article  CAS  Google Scholar 

  27. A. R. LYONS, Current Opin. Orthop. 9 (1998) 2.

    Google Scholar 

  28. P. K. VENESMAA, H. P. J. KROGER, H. J. A. MIETTINEN, J. S. JURVELIN, O. T. SUOMALAINEN and E. M. ALHAVA, J. Bone Miner. Res. 16 (2001) 2126.

    Article  CAS  Google Scholar 

  29. J. M WILKINSON, I. STOCKLEY, N. F. PEEL, A. J. HAMER, R. A. ELSON, N. A. BARRINGTON and R. EASTELL, J. Bone Miner. Res. 16 (2001) 556.

    Article  CAS  Google Scholar 

  30. A. NEHME, G. MAALOUF, J. L. TRICOIRE, G. GIORDANO, P. CHIRON and J. PUGET, Rev. Chir. Orthop. Repar. Appar Mot. 89 (2003) 593.

    CAS  Google Scholar 

  31. M. D. RIES, Instruct. Course Lectures. 52 (2003) 257.

    Google Scholar 

  32. D. E. HUGHES, K. R. WRIGHT, H. L. UY, A. SASAKI, Y. YONEDA, G. D. ROODMAN, G. R. MUNDY and B. F. BOYCE, J. Bone Miner. Res. 10 (1995) 1478.

    Article  CAS  Google Scholar 

  33. G. WILLMANN, Orthopedics. 21 (1998) 173.

    CAS  Google Scholar 

  34. P. J. RICHARDS, A. S. WILLIAMS, R. W. GOODFELLOW and B. D. WILLIAMS, Rheumatol. (Oxford). 38 (1999) 818.

    Article  CAS  Google Scholar 

  35. P. OELZNER, A. KUNZE, S. HENZGEN, K. THOSS, G. HEIN, G. STEIN and R. BRAUER, Inflamm. Res. 49 (2000) 424.

    Article  CAS  Google Scholar 

  36. D. W. HOWIE, D. R. HAYNES, S. D. ROGERS, M. A. MCGEE and M. J. PEARCY, Orthop. Clinics North Am. 24 (1993) 571.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Orthopaedic Surgery, McGill University, Lady Davis Institute for Medical Research, The Sir Mortimer B. Davis—Jewish General Hospital, 3755 Chemin de la Cote Ste-Catherine, Montreal, QC, H3 T 1 E2, Canada

    Alain Petit, Fackson Mwale, John Antoniou, David J. Zukor & Olga L. Huk

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  1. Alain Petit
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  2. Fackson Mwale
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  3. John Antoniou
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  5. Olga L. Huk
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Correspondence to Olga L. Huk.

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Petit, A., Mwale, F., Antoniou, J. et al. Effect of bisphosphonates on the stimulation of macrophages by alumina ceramic particles: A comparison with ultra-high-molecular-weight polyethylene. J Mater Sci: Mater Med 17, 667–673 (2006). https://doi.org/10.1007/s10856-006-9230-x

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  • DOI: https://doi.org/10.1007/s10856-006-9230-x

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