Abstract
Marine mussels produce an impressive adhesive material for affixing themselves to rocks in the turbulent marine environment. This glue is generated by application of proteins to the surface followed by extensive cross-linking to yield the final matrix. Prior studies have shown that simple oxidation or reactivity brought about by metal ions may be key to this protein cross-linking process. Here we have explored protein cross-linking reactivity in which combinations of metals and oxidants may display synergistic effects with respect to adhesive curing. Extracted adhesive proteins were mixed with a series of metals, oxidants, and combinations thereof. In some cases, synergistic curing was observed. For example, we found that iron(II) ions with hydrogen peroxide brought about a greater degree of protein cross-linking than the sum of the individual components. These studies were performed as part of our efforts to provide perspectives on the connections between biology, chemistry, and functional materials.
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Naldrett MJ, Kaplan DL (1997) Marine Biol 127:629
Naldrett MJ (1993) J Mar Biol Assoc UK 73:689
Dougherty WJ (1990) J Crustacean Biol 10:469
Kamino K, Inoue K, Maruyama T, Takamatsu N, Harayama S, Shizuri Y (2000) J Biol Chem 275:27360
Kamino K, Odo S, Maruyama Y (1996) Biol Bull 190:403
Yamamoto H, Nagai A (1992) Marine Chem 37:131
Deming TJ (1999) Curr Opin Chem Biol 3:100
Waite JH, Andersen NH, Jewhurst S, Sun C (2005) J Adhes 81:297
Waite JH (2002) Integr Comp Biol 42:1172
Waite JH, Lichtenegger HC, Stucky GD, Hansma P (2004) Biochemistry 43:7653
Coombs TL, Keller PJ (1981) Aquat Toxicol 1:291
George SG, Pirie BJS, Coombs TL (1976) J Exp Mar Biol 23:71
Pentreath RJ (1973) J Mar Biol Assoc UK 53:127
Hobden DJ (1967) J Mar Biol Assoc UK 47:597
Szefer P, Geldon J, Ahmed-Ali A, Osuna FP, Ruiz-Fernandes AC, Galvan SRG (1998) Environ Int 24:359
Koide M, Lee DS, Goldberg ED (1982) Estuar Coastal Shelf Sci 15:679
Fant C, Elwing H, Hook F (2002) Biomacromolecules 3:732
Fant C, Sott K, Elwing H, Hook F (2000) Biofouling 16:119
Suci PA, Geesey GG (2000) J Colloid Interface Sci 230:340
Hansen DC, Corcoran SG, Waite JH (1998) Langmuir 14:1139
Burzio LA, Burzio VA, Pardo L, Burzio LO (2000) Comp Biochem Physiol B 126:383
Lee H, Scherer NF, Messersmith PB (2006) Proc Natl Acad Sci USA 103:12999
Loizou E, Weisser JT, Dundigalla A, Porcar L, Schmidt G, Wilker JJ (2006) Macromol Biosci 6:711
Weisser JT, Nilges MJ, Sever MJ, Wilker JJ (2006) Inorg Chem 45:7736
Sever MJ, Wilker JJ (2006) Dalton Trans 813
Sever MJ, Weisser JT, Monahan J, Srinivasan S, Wilker JJ (2004) Angew Chem Int Ed 43:448
Monahan J, Wilker JJ (2004) Langmuir 20:3724
Monahan J, Wilker JJ (2003) Chem Commun 1672
Taylor SW, Luther GW III, Waite JH (1994) Inorg Chem 33:5819
Taylor SW, Chase DB, Emptage MH, Nelson MJ, Waite JH (1996) Inorg Chem 35:7572
Frank BP, Belfort G (2001) Langmuir 17:1905
Frank BP, Belfort G (2002) Biotechnol Prog 18:580
Waite JH (1992) Biol Bull 183:178
Rzepecki LM, Waite JH (1991) In: Scheuer PJ (ed) Bioorganic marine chemistry. Springer-Verlag
Denisov IG, Makris TM, Sligar SG, Schlichting I (2005) Chem Rev 105:2253
Gozzo F (2001) J Mol Catal A 171:1
Waite JH (1985) J Mar Biol Assoc UK 65:359
Mishra B, Hassan PA, Priyadarsini KI, Mohan H (2005) J Phys Chem B 109:12718
Halliwell B (1990) Free Radic Res Commun 9:1
Sunda WG (2001) In: Turner DR, Hunter KA (ed) The biogeochemistry of iron in seawater. Wiley
Ussher SJ, Achterberg EP, Worsfold PJ (2004) Environ Chem 1:67
Achterberg EP, Holland TW, Bowie AR, Fauzi R, Mantoura C, Worsfold PJ (2001) Anal Chim Acta 442:1
Richardson DR (2005) Curr Med Chem 12:2711
Lide DR (1992) CRC handbook of chemistry and physics, 73rd edn. CRC Press, Boca Raton
Bratsch SG (1989) J Phys Chem Ref Data 18:1
Waite JH (1995) Methods Enzymol 258:1
Bourne MC (1966) J Food Sci 31:282
Bourne MC (1967) J Food Sci 32:601
Bourne MC (1975) J Texture Stud 5:459
Bourne MC (1982) In: Food texture and viscosity: concept and measurement. New York
deMan JM (1969) J Texture Stud 1:114
Sever MJ, Wilker JJ (2004) Dalton Trans 1061
Decker H, Tuczek F (2000) Trends Biochem Sci 25:392
Balla J, Kiss T, Jameson RF (1992) Inorg Chem 31:58
Bard AJ, Parsons R, Jordan J (1985) In: Standard potentials in aqueous solution. New York
Donat JR, Bruland KW (1995) In: Salbu B, Steinnes E (ed) Trace elements in natural waters. CRC Press
Crichton RR, Wilmet S, Legssyer R, Ward RJ (2002) J Inorg Biochem 91:9
Acknowledgements
LMH was a summer researcher in the Purdue University Chemical Biology Research Experience for Undergraduates program, sponsored by the National Science Foundation. This work was also supported by the Lord Corporation and the Office of Naval Research.
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Hight, L.M., Wilker, J.J. Synergistic effects of metals and oxidants in the curing of marine mussel adhesive. J Mater Sci 42, 8934–8942 (2007). https://doi.org/10.1007/s10853-007-1648-0
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DOI: https://doi.org/10.1007/s10853-007-1648-0