Abstract
The phototoxicity potential of pyrene on natural algae and bacteria in an arctic sediment was evaluated and compared to that of pyrene treatment alone based on some functional and structural endpoints. Microcosms with arctic sediment from a shallow-water marine bay were incubated with pyrene under three different light regimes, natural sunlight with UV-light (LightUV), natural sunlight without UV-light (Light) and dark.
Presence of pyrene directly affected the algal community measured as decreased 14C-incorporation and decreased ammonium, nitrate and silicate uptake. These direct toxic effects from pyrene on the algae eventually led to indirect effects on the bacterial community observed as increased oxygen consumption.
Besides the direct toxicity of pyrene to the benthic microbial community, indications of phototoxicity were found on the bacterial community detected as decreased oxygen consumption and increased bacterial diversity under LightUV compared to Light. No indication of phototoxicity of pyrene was found on the algae, which might be due to the high direct toxicity of pyrene. Our results indicate that shallow arctic marine areas might be affected by phototoxicity if concentrations of oil components in the sediments increase.
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References
Chapman PM, Riddle MJ (2003) Missing and needed: Polar marine ecotoxicology. Mar Poll Bull 46:927–928
Chapman PM, Riddle MJ (2005) Toxic effects of contaminants in polar marine environments. Environ Sci Technol 39:200A–207A
Dahllof I, Blanck H, Hall P (1999) Short-term effects of tri-n-butyl-tin on marine sediment samples using nutrient fluxes as effect indicators. Environ Toxicol Chem 18:850–857
Diamond SA (2003) Photoactivated toxicity in aquatic environments. In: Helbling EW, Zagarese HE (eds) UV effects in aquatic organisms and ecosystems. Royal Society of Chemistry, Cambridge, UK
Dixon WJ, Massey FJ (1983) Detection of extremes. In: Corrigan JJ, Wagley S, Amar JS (eds) Introduction to statistical analysis. McGraw-Hill, New York, USA
Djomo JE, Dauta A, Ferrier V, Narbonne JF, Monkiedje A, Njine T, Garrigues P (2004) Toxic effects of some major polyaromatic hydrocarbons found in crude oil and aquatic sediments on Scenedesmus subspicatus. Water Res 38:1817–1821
El-Alawi YS, Dixon DG, Greenberg BM (2001) Effects of a pre-incubation period on the photoinduced toxicity of polycyclic aromatic hydrocarbons to the luminescent bacterium Vibrio fischeri. Environ Toxicol 16:277–286
Huang XD, Krylov SN, Ren LS, McConkey BJ, Dixon DG, Greenberg BM, (1997) Mechanistic quantitative structure-activity relationship model for the photoinduced toxicity of polycyclic aromatic hydrocarbons. 2. An empirical model for the toxicity of 16 polycyclic aromatic hydrocarbons to the duckweed Lemna gibba L. G-3. Environ Toxicol Chem 16:2296–2303
Hwang HM, McCullum D, Slaughter L (1998) Phototransformation of 2,4−dichloroaniline in a surface freshwater environment: Effects on microbial assemblages. B. Environ Contam Toxic 60:81–87
Krylov SN, Huang XD, Zeiler LF, Dixon DG, Greenberg BM (1997) Mechanistic quantitative structure-activity relationship model for the photoinduced toxicity of polycyclic aromatic hydrocarbons .1. Physical model based on chemical kinetics in a two-compartment system. Environ Toxicol Chem 16:2283–2295
Manoli E, Samara C (1999) Polycyclic aromatic hydrocarbons in natural waters: sources, occurrence and analysis. Trac-Trend Anal Chem 18:417–428
McDonald BG, Chapman PM (2002) PAH phototoxicity–an ecologically irrelevant phenomenon? Mar Poll Bull 44:1321–1326
Neff JM (1979) Polycyclic aromatic hydrocarbons in the aquatic environment. Applied Science publishers LTD, Essex, England
Pelletier MC, Burgess RM, Ho KT, Kuhn A, McKinney RA, Ryba SA (1997) Phototoxicity of individual polycyclic aromatic hydrocarbons and petroleum to marine invertebrate larvae and juveniles. Environ Toxicol Chem 16:2190–2199
Petersen DG, Dahllof I (2005) Improvements for comparative analysis of changes in diversity of microbial communities using internal standards in PCR-DGGE. FEMS Microbiol Ecol 53:339–348
Petersen DG, Dahllof I, Nielsen LP (2004) Effects of zinc pyrithione and copper pyrithione on microbial community function and structure in sediments. Environ Toxicol Chem 23:921–928
Riget F, Law RJ, Hansen JC (2004) The state of contaminants in the Greenland environment. Sci Total Environ 331:1–4
Romanowski G, Lorenz MG, Wackernagel W (1993) Use of polymerase chain-reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils. Appl Environ Microbiol 59:3438–3446
Sargian P, Mostajir B, Chatila K, Ferreyra GA, Pelletier E, Demers S (2005) Non-synergistic effects of water-soluble crude oil and enhanced ultraviolet-B radiation on a natural plankton assemblage. MEPS 294:63–77
Shindell DT, Rind D, Lonergan P (1998) Increased polar stratospheric ozone losses and delayed eventual recovery owing to increasing greenhouse-gas concentrations. Nature 392:589–592
Shugart LR, Theodorakis C (1996) Genetic ecotoxicology: The genotypic diversity approach. Comp Biochem Physiol Part C Pharmacol Toxicol Endocrinol 113:273–276
Sverdrup LE, Ekelund F, Krogh PH, Nielsen T, Johnsen K (2002) Soil microbial toxicity of eight polycyclic aromatic compounds: Effects on nitrification, the genetic diversity of bacteria, and the total number of protozoans. Environ Toxicol Chem 21:1644–1650
Verrhiest GJ, Clement B, Volat B, Montuelle B, Perrodin Y (2002) Interactions between a polycyclic aromatic hydrocarbon mixture and the microbial communities in a natural freshwater sediment. Chemosphere 46:187–196
Warshawsky D, Cody T, Radike M, Reilman R, Shumann B, Ladow K, Schneider J (1995) Biotransformation of benzo[a]pyrene and other polycyclic aromatic-hydrocarbons and heterocyclic-analogs by several green-algae and other algal species under gold and white-light. Chem-Biol Interact 97:131–148
Weinstein JE, Polk KD (2001) Phototoxicity of anthracene and pyrene to glochidia of the freshwater mussel Utterbackia imbecillis. Environ Toxicol Chem 20:2021–2028
Acknowledgements
The staff and Professor Arne Willumsen from The Centre for Arctic Technology at The Danish Technical University are kindly thanked for letting us join their group to Greenland and for letting us use the laboratory facilities at the Sanaartornermik Ilinniarfik school. We wish to thank Knud Højgaards Foundation and The Letterstedtske Society for financial support and the anonymous reviewers for helpful comments on the manuscript. Disclaimer The conclusions of this work are solely the authors and not those of the organisation. There are no financial or ethical conflicts in the work performed.
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Petersen, D.G., Dahllöf, I. Combined effects of pyrene and UV-light on algae and bacteria in an arctic sediment. Ecotoxicology 16, 371–377 (2007). https://doi.org/10.1007/s10646-007-0139-z
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DOI: https://doi.org/10.1007/s10646-007-0139-z