Abstract
Topmouth culter (Culter alburnus) is an economically important fish species distributed in rivers, lakes and reservoirs throughout China. This study focused on the genetic diversity and genetic germplasm resources of C. alburnus populations. We developed a set of 14 polymorphic multi-nucleotide repeat microsatellites using specific locus amplification fragment sequencing (SLAF-seq). Based on microsatellites and mitochondrial DNA D-loop sequences analysis, we identified six C. alburnus populations that had a high level of genetic diversity. Among them, the Xingkai Lake (XKL) population had the lowest genetic diversity, so it might require strengthened protection measures. XKL and Danjiangkou Reservoir (DJKR) populations were relatively closely related, and genetically distant from the remaining four populations. These results shall help us effectively monitor the germplasm resources of C. alburnus and provide scientific guidance for its resource development, utilization and protection.
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Data availability
The datasets presented in this study can be found in NCBI repositories. The accession numbers can be found in the article.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (grants No. 31602163), Wuhan Youth Science and Technology Plan (grant No. 2015071704011613), and Hubei Natural Science Foundation Project (grant No. 2018CFB684). We are grateful to Dr. Ivan Jakovlić from the Bio-Transduction Lab (Biolake, Wuhan) for proofreading and editing the manuscript.
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Ning Sun, do experiments, write the first draft of the paper. Dongmei Zhu, funding, revise the paper. Qing Li, funding, modify the experimental scheme. Guiying Wang, sample. Jian Chen, sample. Feifei Zheng, analyse SSR data. Pei Li, sample. Yanhong Sun, analyse D-loop data.
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Sun, N., Zhu, DM., Li, Q. et al. Genetic diversity analysis of Topmouth Culter (Culter alburnus) based on microsatellites and D-loop sequences. Environ Biol Fish 104, 213–228 (2021). https://doi.org/10.1007/s10641-021-01062-2
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DOI: https://doi.org/10.1007/s10641-021-01062-2