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In vivo Characterization of Brain Morphometric and Metabolic Endophenotypes in Three Inbred Strains of Mice Using Magnetic Resonance Techniques

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Abstract

C57BL/6J, FVB/N and 129/SvJ mice are commonly used as background strains to engineer genetic models of brain pathologies and psychiatric disorders. Magnetic resonance imaging (MRI) and spectroscopy provide alternative approaches to neuroanatomy, histology and neurohistochemistry for investigating the correlation between genes and brain neuroanatomy and neurometabolism in vivo. We used these techniques to non-invasively characterize the cerebral morphologic and metabolic endophenotypes of inbred mouse strains commonly used in neurological and behavioral research. We observed a great variability in the volume of ventricles and of structures involved in cognitive function (cerebellum and hippocampus) among these strains. In addition, distinct metabolic profiles were evidenced with variable levels of N-acetylaspartate, a neuronal marker, and of choline, a compound found in membranes and myelin. Besides, significant differences in high-energy phosphates and phospholipids were detected. Our findings demonstrate the great morphologic and metabolic heterogeneity among C57BL/6J, FVB/N and 129/SvJ mice. They emphasize the importance of selecting the appropriate genetic background for over-expressing or silencing a gene and provide some directions for modeling symptoms that characterize psychiatric disorders such as autism, schizophrenia and depression.

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Abbreviations

B6:

C57BL/6J

Cho:

Choline

tCr:

total creatine

FVB:

FVB/N

Glx:

glutamate + glutamine

MRI:

magnetic resonance imaging

MRS:

magnetic resonance spectroscopy

myo-Ins:

myo-Inositol

NAA:

N-acetylaspartate

NAAG:

N-acetylaspartylglutamate

Pi:

inorganic phosphate

PCr:

phosphocreatine

PDE:

phosphodiesters

PME:

phosphomonoesters

TE:

time of echo

129:

129/ SvJ

References

  • Alexander JJ, Zwingmann C, Quigg R (2005) MRL/lpr mice have alterations in brain metabolism as shown with [1H–13C] NMR spectroscopy. Neurochem Int 47:143–151

    Article  PubMed  CAS  Google Scholar 

  • Anagnostopoulos AV, Mobraaten LE, Sharp JJ, Davisson MT (2001) Transgenic and knockout databases: behavioral profiles of mouse mutants. Physiol Behav 73:675–689

    Article  PubMed  CAS  Google Scholar 

  • Antonova E, Sharma T, Morris R, Kumari V (2004) The relationship between brain structure and neurocognition in schizophrenia: a selective review. Schizophr Res 70:117–145

    Article  PubMed  Google Scholar 

  • Arnold DL, Matthews PM, Radda GK (1984) Metabolic recovery after exercise and the assessment of mitochondrial function in vivo in human skeletal muscle by means of 31P NMR. Magn Reson Med 1:307–315

    Article  PubMed  CAS  Google Scholar 

  • Baslow MH (2002) Evidence supporting a role for N-acetyl-l-aspartate as a molecular water pump in myelinated neurons in the central nervous system. An analytical review. Neurochem Int 40:295–300

    Article  PubMed  CAS  Google Scholar 

  • Baslow MH (2003) N-acetylaspartate in the vertebrate brain: metabolism and function. Neurochem Res 28:941–953

    Article  PubMed  CAS  Google Scholar 

  • Bauman ML, Kemper TL (2005) Neuroanatomic observations of the brain in autism: a review and future directions. Int J Dev Neurosci 23:183–187

    Article  PubMed  Google Scholar 

  • Belichenko PV, Masliah E, Kleschevnikov AM, Villar AJ, Epstein CJ, Salehi A, Mobley WC (2004) Synaptic structural abnormalities in the Ts65Dn mouse model of Down Syndrome. J Comp Neurol 480:281–298

    Article  PubMed  Google Scholar 

  • Brambilla P, Hardan A, di Nemi SU, Perez J, Soares JC, Barale F (2003) Brain anatomy and development in autism: review of structural MRI studies. Brain Res Bull 61:557–569

    Article  PubMed  Google Scholar 

  • Carper RA, Courchesne E (2005) Localized enlargement of the frontal cortex in early autism. Biol Psychiatry 57:126–133

    Article  PubMed  Google Scholar 

  • Choi IY, Lee SP, Guilfoyle DN, Helpern JA (2003) In vivo NMR studies of neurodegenerative diseases in transgenic and rodent models. Neurochem Res 28:987–1001

    Article  PubMed  CAS  Google Scholar 

  • Confort-Gouny S, Vion-Dury J, Nicoli F, Dano P, Donnet A, Grazziani N, Gastaut JL, Grisoli F, Cozzone PJ (1993) A multiparametric data analysis showing the potential of localized proton MR spectroscopy of the brain in the metabolic characterization of neurological diseases. J Neurol Sci 118:123–133

    Article  PubMed  CAS  Google Scholar 

  • Courchesne E, Saitoh O, Townsend JP, Yeung-Courchesne R, Press GA, Lincoln AJ, Haas RH, Schriebman L (1994) Cerebellar hypoplasia and hyperplasia in infantile autism. Lancet 343:63–64

    Article  PubMed  CAS  Google Scholar 

  • Cowan WM, Kopnisky KL, Hyman SE (2002) The human genome project and its impact on psychiatry. Annu Rev Neurosci 25:1–50

    Article  PubMed  CAS  Google Scholar 

  • Crnic L, Pennington B (2000) Down syndrome: neuropsychology and animal model. Prog Infan Res 1:61–111

    Google Scholar 

  • Csernansky JG, Wang L, Swank J, Miller JP, Gado M, McKeel D, Miller MI, Morris JC (2005) Preclinical detection of Alzheimer’s disease: hippocampal shape and volume predict dementia onset in the elderly. Neuroimage 25:783–792

    Article  PubMed  CAS  Google Scholar 

  • Dager SR, Friedman SD, Heide A, Layton ME, Richards T, Artru A, Strauss W, Hayes C, Posse S (1999) Two-dimensional proton echo-planar spectroscopic imaging of brain metabolic changes during lactate-induced panic. Arch Gen Psychiatry 56:70–77

    Article  PubMed  CAS  Google Scholar 

  • Dager SR, Strauss WL, Marro KI, Richards TL, Metzger GD, Artru AA (1995) Proton magnetic resonance spectroscopy investigation of hyperventilation in subjects with panic disorder and comparison subjects. Am J Psychiatry 152:666–672

    PubMed  CAS  Google Scholar 

  • Dierssen M, Fotaki V, Martinez de Lagran M, Gratacos M, Arbones M, Fillat C, Estivill X (2002) Neurobehavioral development of two mouse lines commonly used in transgenic studies. Pharmacol Biochem Behav 73:19–25

    Article  PubMed  CAS  Google Scholar 

  • Evans KL, Muir WJ, Blackwood DH, Porteous DJ (2001) Nuts and bolts of psychiatric genetics: building on the Human Genome Project. Trends Genet 17:35–40

    Article  PubMed  CAS  Google Scholar 

  • Fava M, Kendler KS (2000) Major depressive disorder. Neuron 28:335–341

    Article  PubMed  CAS  Google Scholar 

  • Fiehn O (2002) Metabolomics—the link between genotypes and phenotypes. Plant Mol Biol 48:155–171

    Article  PubMed  CAS  Google Scholar 

  • Flint J, Wilkie AO, Buckle VJ, Winter RM, Holland AJ, McDermid HE (1995) The detection of subtelomeric chromosomal rearrangements in idiopathic mental retardation. Nat Genet 9:132–140

    Article  PubMed  CAS  Google Scholar 

  • Frangou S, Chitins X, Williams SC (2004) Mapping IQ and gray matter density in healthy young people. Neuroimage 23:800–805

    Article  PubMed  Google Scholar 

  • Galanaud D, Nicoli F, Le Fur Y, Guye M, Ranjeva JP, Confort-Gouny S, Viout P, Soulier E, Cozzone PJ (2003) Multimodal magnetic resonance imaging of the central nervous system. Biochimie 85:905–914

    Article  PubMed  CAS  Google Scholar 

  • Ganesh S, Delgado-Escueta AV, Sakamoto T, Avila MR, Machado-Salas J, Hoshii Y, Akagi T, Gomi H, Suzuki T, Amano K, Agarwala KL, Hasegawa Y, Bai DS, Ishihara T, Hashikawa T, Itohara S, Cornford EM, Niki H, Yamakawa K (2002) Targeted disruption of the Epm2a gene causes formation of Lafora inclusion bodies, neurodegeneration, ataxia, myoclonus epilepsy and impaired behavioral response in mice. Hum Mol Genet 11:1251–1262

    Article  PubMed  CAS  Google Scholar 

  • Gill SS, Small RK, Thomas DG, Patel P, Porteous R, Van Bruggen N, Gadian DG, Kauppinen RA, Williams SR (1989) Brain metabolites as 1H NMR markers of neuronal and glial disorders. NMR Biomed 2:196–200

    Article  PubMed  CAS  Google Scholar 

  • Gordon JA, Hen R (2004) Genetic approaches to the study of anxiety. Annu Rev Neurosci 27:193–222

    Article  PubMed  CAS  Google Scholar 

  • Green EL (1966) Biology of the laboratory mouse. McGraw-Hill, New-York

    Google Scholar 

  • Green EL (1981) Genetics and probability in animal breeding experiments. MacMillan, London

    Google Scholar 

  • Griffin JL (2004) Metabolic profiles to define the genome: can we hear the phenotypes? Philos Trans R Soc Lond B Biol Sci 359:857–871

    Article  PubMed  CAS  Google Scholar 

  • Griffin JL, Cemal CK, Pook MA (2004) Defining a metabolic phenotype in the brain of a transgenic mouse model of spinocerebellar ataxia 3. Physiol Genomics 16:334–340

    Article  PubMed  CAS  Google Scholar 

  • Guimera J, Casas C, Pucharcos C, Solans A, Domenech A, Planas AM, Ashley J, Lovett M, Estivill X, Pritchard MA (1996) A human homologue of Drosophila minibrain (MNB) is expressed in the neuronal regions affected in Down syndrome and maps to the critical region. Hum Mol Genet 5:1305–1310

    Article  PubMed  CAS  Google Scholar 

  • Heintz N, Norman D, Gao W-Q, Hatten M (1993) Neurogenetic approaches to mammalian brain development. In: Tilghman Da (ed) Genome maps and neurological disorders. Cold Spring Harbor Press, Cold Spring Harbor, pp 19–44

    Google Scholar 

  • Hock C, Konietzko U, Streffer JR, Tracy J, Signorell A, Muller-Tillmanns B, Lemke U, Henke K, Moritz E, Garcia E, Wollmer MA, Umbricht D, de Quervain DJ, Hofmann M, Maddalena A, Papassotiropoulos A, Nitsch RM (2003) Antibodies against beta-amyloid slow cognitive decline in Alzheimer’s disease. Neuron 38:547–554

    Article  PubMed  CAS  Google Scholar 

  • Holowenko D, Peeling J, Sutherland G (1992) 1H NMR properties of N-acetylaspartylglutamate in extracts of nervous tissue of the rat. NMR Biomed 5:43–47

    Article  PubMed  CAS  Google Scholar 

  • Hoogenraad CC, Koekkoek B, Akhmanova A, Krugers H, Dortland B, Miedema M, van Alphen A, Kistler WM, Jaegle M, Koutsourakis M, Van Camp N, Verhoye M, van der Linden A, Kaverina I, Grosveld F, De Zeeuw CI, Galjart N (2002) Targeted mutation of Cyln2 in the Williams syndrome critical region links CLIP-115 haploinsufficiency to neurodevelopmental abnormalities in mice. Nat Genet 32:116–127

    Article  PubMed  CAS  Google Scholar 

  • Hyman S (2000) Mental illness: genetically complex disorders of neural circuitry and neural communication. Neuron 28:321–323

    Article  PubMed  CAS  Google Scholar 

  • Jayakumar PN, Gangadhar BN, Subbakrishna DK, Janakiramaiah N, Srinivas JS, Keshavan MS (2003) Membrane phospholipid abnormalities of basal ganglia in never-treated schizophrenia: a 31P magnetic resonance spectroscopy study. Biol Psychiatry 54:491–494

    Article  PubMed  CAS  Google Scholar 

  • Jenkins BG, Klivenyi P, Kustermann E, Andreassen OA, Ferrante RJ, Rosen BR, Beal MF (2000) Nonlinear decrease over time in N-acetyl aspartate levels in the absence of neuronal loss and increases in glutamine and glucose in transgenic Huntington’s disease mice. J Neurochem 74:2108–2119

    Article  PubMed  CAS  Google Scholar 

  • Jensen JE, Miller J, Williamson PC, Neufeld RW, Menon RS, Malla A, Manchanda R, Schaefer B, Densmore M, Drost DJ (2004) Focal changes in brain energy and phospholipid metabolism in first-episode schizophrenia: 31P-MRS chemical shift imaging study at 4 Tesla. Br J Psychiatry 184:409–415

    Article  PubMed  Google Scholar 

  • Kandel ER, Squire LR (2000) Neuroscience: breaking down scientific barriers to the study of brain and mind. Science 290:1113–1120

    Article  PubMed  CAS  Google Scholar 

  • Kesner RP, Lee I, Gilbert P (2004) A behavioral assessment of hippocampal function based on a subregional analysis. Rev Neurosci 15:333–351

    PubMed  Google Scholar 

  • Kreis R, Ernst T, Ross BD (1993) Development of the human brain: in vivo quantification of metabolite and water content with proton magnetic resonance spectroscopy. Magn Reson Med 30:424–437

    Article  PubMed  CAS  Google Scholar 

  • Kreis R, Hofmann L, Kuhlmann B, Boesch C, Bossi E, Huppi PS (2002) Brain metabolite composition during early human brain development as measured by quantitative in vivo 1H magnetic resonance spectroscopy. Magn Reson Med 48:949–958

    Article  PubMed  CAS  Google Scholar 

  • Lalonde R, Strazielle C (2003) The effects of cerebellar damage on maze learning in animals. Cerebellum 2:300–309

    Article  PubMed  CAS  Google Scholar 

  • Lei H, Zhang Y, Zhu XH, Chen W (2003) Changes in the proton T2 relaxation times of cerebral water and metabolites during forebrain ischemia in rat at 9.4 T. Magn Reson Med 49:979–984

    Article  PubMed  CAS  Google Scholar 

  • Lewis DA, Levitt P (2002) Schizophrenia as a disorder of neurodevelopment. Annu Rev Neurosci 25:409–432

    Article  PubMed  CAS  Google Scholar 

  • Lewis DA, Lieberman JA (2000) Catching up on schizophrenia: natural history and neurobiology. Neuron 28:325–334

    Article  PubMed  CAS  Google Scholar 

  • Manton DJ, Lowry M, Blackband SJ, Horsman A (1995) Determination of proton metabolite concentrations and relaxation parameters in normal human brain and intracranial tumours. NMR Biomed 8:104–112

    Article  PubMed  CAS  Google Scholar 

  • Matynia A, Kushner SA, Silva AJ (2002) Genetic approaches to molecular and cellular cognition: a focus on LTP and learning and memory. Annu Rev Genet 36:687–720

    Article  PubMed  CAS  Google Scholar 

  • Mayford M, Mansuy IM, Muller RU, Kandel ER (1997) Memory and behavior: a second generation of genetically modified mice. Curr Biol 7:R580–R589

    Article  PubMed  CAS  Google Scholar 

  • Middleton FA, Strick PL (1998a) Cerebellar output: motor and cognitive channels. Trends Cognit Sci 2:348–354

    Article  Google Scholar 

  • Middleton FA, Strick PL (1998b) The cerebellum: an overview. Trends Neurosci 21:367–369

    Article  CAS  Google Scholar 

  • Milner B, Squire LR, Kandel ER (1998) Cognitive neuroscience and the study of memory. Neuron 20:445–468

    Article  PubMed  CAS  Google Scholar 

  • Minshew NJ, Goldstein G, Dombrowski SM, Panchalingam K, Pettegrew JW (1993) A preliminary 31P MRS study of autism: evidence for undersynthesis and increased degradation of brain membranes. Biol Psychiatry 33:762–773

    Article  PubMed  CAS  Google Scholar 

  • Moolenaar SH, Engelke UF, Wevers RA (2003) Proton nuclear magnetic resonance spectroscopy of body fluids in the field of inborn errors of metabolism. Ann Clin Biochem 40:16–24

    Article  PubMed  CAS  Google Scholar 

  • Morley KI, Montgomery GW (2001) The genetics of cognitive processes: candidate genes in humans and animals. Behav Genet 31:511–531

    Article  PubMed  CAS  Google Scholar 

  • Mouse-Genome-Sequencing-Consortium (2002) Initial sequencing and comparative analysis of the mouse genome. Nature 420:520–562

    Article  CAS  Google Scholar 

  • Neiderhiser JM, Plomin R, McClearn GE (1992) The use of CXB recombinant inbred mice to detect quantitative trait loci in behavior. Physiol Behav 52:429–439

    Article  PubMed  CAS  Google Scholar 

  • Nicholson JK, Connelly J, Lindon JC, Holmes E (2002) Metabonomics: a platform for studying drug toxicity and gene function. Nat Rev Drug Discov 1:153–161

    Article  PubMed  CAS  Google Scholar 

  • O’Keefe J, Nadel L (1978) The hippocampus as a cognitive map. Oxford University Press, Oxford

    Google Scholar 

  • Pandurangi AK, Dewan MJ, Lee SH, Ramachandran T, Levy BF, Boucher M, Yozawitz A, Major L (1984) The ventricular system in chronic schizophrenic patients. A controlled computed tomography study. Br J Psychiatry 144:172–176

    Article  PubMed  CAS  Google Scholar 

  • Passani LA, Vonsattel JP, Carter RE, Coyle JT (1997) N-acetylaspartylglutamate, N-acetylaspartate, and N-acetylated alpha-linked acidic dipeptidase in human brain and their alterations in Huntington and Alzheimer’s diseases. Mol Chem Neuropathol 31:97–118

    Article  PubMed  CAS  Google Scholar 

  • Pellerin L, Magistretti PJ (2004) Neuroenergetics: calling upon astrocytes to satisfy hungry neurons. Neuroscientist 10:53–62

    Article  PubMed  CAS  Google Scholar 

  • Penet MF, Viola A, Confort-Gouny S, Le Fur Y, Duhamel G, Kober F, Ibarrola D, Izquierdo M, Coltel N, Gharib B, Grau GE, Cozzone PJ (2005) Imaging experimental cerebral malaria in vivo: significant role of ischemic brain edema. J Neurosci 25:7352–7358

    Article  PubMed  CAS  Google Scholar 

  • Pettegrew JW, Panchalingam K, Klunk WE, McClure RJ, Muenz LR (1994) Alterations of cerebral metabolism in probable Alzheimer’s disease: a preliminary study. Neurobiol Aging 15:117–132

    Article  PubMed  CAS  Google Scholar 

  • Pouwels PJ, Frahm J (1997) Differential distribution of NAA and NAAG in human brain as determined by quantitative localized proton MRS. NMR Biomed 10:73–78

    Article  PubMed  CAS  Google Scholar 

  • Raz S, Raz N (1990) Structural brain abnormalities in the major psychoses: a quantitative review of the evidence from computerized imaging. Psychol Bull 108:93–108

    Article  PubMed  CAS  Google Scholar 

  • Remy C, Albrand JP, Benabid AL, Decorps M, Jacrot M, Riondel J, Foray MF (1987) In vivo 31P nuclear magnetic resonance studies of T 1 and T 2 relaxation times in rat brain and in rat brain tumors implanted to nude mice. Magn Reson Med 4:144–152

    Article  PubMed  CAS  Google Scholar 

  • Rodriguez LA, Plomin R, Blizard DA, Jones BC, McClearn GE (1995) Alcohol acceptance, preference, and sensitivity in mice. II. Quantitative trait loci mapping analysis using BXD recombinant inbred strains. Alcohol Clin Exp Res 19:367–373

    Article  PubMed  CAS  Google Scholar 

  • Ross B, Bluml S (2001) Magnetic resonance spectroscopy of the human brain. Anat Rec 265:54–84

    Article  PubMed  CAS  Google Scholar 

  • Schmahmann J (1998) Dysmetria of thought; clinical consequences of cerebella dysfunction on cognistion and affect. Trends Cognit Sci 2:362–371

    Article  Google Scholar 

  • Schwarcz A, Natt O, Watanabe T, Boretius S, Frahm J, Michaelis T (2003) Localized proton MRS of cerebral metabolite profiles in different mouse strains. Magn Reson Med 49:822–827

    Article  PubMed  CAS  Google Scholar 

  • Shin JC, Ivry RB (2003) Spatial and temporal sequence learning in patients with Parkinson’s disease or cerebellar lesions. J Cogn Neurosci 15:1232–1243

    Article  PubMed  Google Scholar 

  • Simpson EM, Linder CC, Sargent EE, Davisson MT, Mobraaten LE, Sharp JJ (1997) Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat Genet 16:19–27

    Article  PubMed  CAS  Google Scholar 

  • Styner M, Lieberman JA, McClure RK, Weinberger DR, Jones DW, Gerig G (2005) Morphometric analysis of lateral ventricles in schizophrenia and healthy controls regarding genetic and disease-specific factors. Proc Natl Acad Sci USA 102:4872–4877

    Article  PubMed  CAS  Google Scholar 

  • Taketo M, Schroeder AC, Mobraaten LE, Gunning KB, Hanten G, Fox RR, Roderick TH, Stewart CL, Lilly F, Hansen CT et al. (1991) FVB/N: an inbred mouse strain preferable for transgenic analyses. Proc Natl Acad Sci USA 88:2065–2069

    Article  PubMed  CAS  Google Scholar 

  • Torres G, Meeder BA, Hallas BH, Spernyak JA, Mazurchuk R, Jones C, Gross KW, Horowitz JM (2005) Ventricular size mapping in a transgenic model of schizophrenia. Brain Res Dev Brain Res 154:35–44

    Article  PubMed  CAS  Google Scholar 

  • Tsaih SW, Lu L, Airey DC, Williams RW, Churchill GA (2005) Quantitative trait mapping in a diallel cross of recombinant inbred lines. Mamm Genome 16:344–355

    Article  PubMed  Google Scholar 

  • Urenjak J, Williams SR, Gadian DG, Noble M (1992) Specific expression of N-acetylaspartate in neurons, oligodendrocyte-type-2 astrocyte progenitors, and immature oligodendrocytes in vitro. J Neurochem 59:55–61

    Article  PubMed  CAS  Google Scholar 

  • Veenstra-Vanderweele J, Christian SL, Cook EH Jr (2004) Autism as a paradigmatic complex genetic disorder. Annu Rev Genomics Hum Genet 5:379–405

    Article  PubMed  CAS  Google Scholar 

  • Viola A, Chabrol B, Nicoli F, Confort-Gouny S, Viout P, Cozzone PJ (2002) Magnetic resonance spectroscopy study of glycine pathways in nonketotic hyperglycinemia. Pediatr Res 52:292–300

    Article  PubMed  CAS  Google Scholar 

  • Viola A, Nicoli F, Denis B, Confort-Gouny S, Le Fur Y, Ranjeva JP, Viout P, Cozzone PJ (2004) High cerebral scyllo-inositol: a new marker of brain metabolism disturbances induced by chronic alcoholism. Magma 17:47–61

    Article  PubMed  CAS  Google Scholar 

  • Wu CL, Melton DW (1993) Production of a model for Lesch–Nyhan syndrome in hypoxanthine phosphoribosyltransferase-deficient mice. Nat Genet 3:235–240

    Article  PubMed  CAS  Google Scholar 

  • Yacubian J, de Castro CC, Ometto M, Barbosa E, de Camargo CP, Tavares H, Cerri GG, Gattaz WF (2002) 31P-spectroscopy of frontal lobe in schizophrenia: alterations in phospholipid and high-energy phosphate metabolism. Schizophr Res 58:117–122

    Article  PubMed  Google Scholar 

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Acknowledgments

This work is supported by grants from CNRS (Centre National de la Recherche Scientifique), Programme National Imagerie du Petit Animal (CNRS, CEA), IUF (Institut Universitaire de France) and Action Concertée Incitative “Plates-formes d’explorations fonctionnelles thématisées” (ACI 2003). The authors are grateful to Michèle Carlier for assistance in statistical analysis.

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  1. Faculté de Médecine, Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Université de la Méditérranée, 27 Bd J. Moulin, Marseille, 13005, France

    Marie-France Penet, Christophe Laigle, Yann Le Fur, Sylviane Confort-Gouny, Patrick J. Cozzone & Angèle Viola

  2. Institut de Pharmacologie Moléculaire et Cellulaire-I.P.M.C, UMR 6097, CNRS-UNSA, Sophia Antipolis, Valbonne, France

    Catherine Heurteaux

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  1. Marie-France Penet
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  2. Christophe Laigle
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  3. Yann Le Fur
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  4. Sylviane Confort-Gouny
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Correspondence to Angèle Viola.

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The first two authors contributed equally to this work.

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Penet, MF., Laigle, C., Fur, Y.L. et al. In vivo Characterization of Brain Morphometric and Metabolic Endophenotypes in Three Inbred Strains of Mice Using Magnetic Resonance Techniques. Behav Genet 36, 732–744 (2006). https://doi.org/10.1007/s10519-006-9077-4

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