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Inhibitory action of purified hydroxytyrosol from stored olive mill waste on intestinal disaccharidases and lipase activities and pancreatic toxicity in diabetic rats

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Abstract

This study was established that after 3 months storage, olive mill waste (OMW) was enriched with hydroxytyrosol (HT) (2,652 mg/L) and contained only phenolic monomers. It also showed that HT supplementation to surviving diabetic rats decreases considerably intestinal maltase, lactase, sucrose, and lipase activities by 49, 31, 42, and 40%, respectively. In addition, HT enhanced the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities by 330, 170, and 301%, respectively and reduced gluthation (GSH) level by 79% in pancreas compared to diabetic rats. Moreover, a significant decrease in the thiobarbituric acid reactive substances (TBARs) level and lactate dehydrogenase (LDH) activity in diabetic rats pancreas by 34 and 49% respectively after administration of HT. In addition, HT improved plasma triglyceride and cholesterol levels in diabetic rats by lowering low density lipoprotein (LDL)-cholesterol and triglycerides (TG) by 27 and 53%, respectively and increasing high density lipoprotein (HDL)-cholesterol content by 72%. These properties of HT are interesting regarding its use as therapeutic agent in biotechnological applications, especially in developing anti-diabetic and hypocholesterolemic drugs.

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Authors and Affiliations

  1. Faculty of Science of Sfax, Laboratory of Animal Ecophysiology, P.O. Box 95, Sfax, 3052, Tunisia

    Khaled Hamden & Abdelfattah Elfeki

  2. Faculty of Sciences of Sfax, Laboratory of Chemistry of Natural Products, B.P. 1171, 3000, Sfax, Tunisia

    Noureddine Allouche & Mohamed Damak

  3. Laboratory of Prokaryotic Enzymes and Metabolites, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax, 3018, Tunisia

    Bessam Jouadi

  4. Faculty of Science of El-Manar I, Laboratory of Physiology of Aggressions, Tunis, 1060, Tunisia

    Saloua El-Fazaa & Najoua Gharbi

  5. Department of Biochemistry, University of Caen-Basse Normandie, USC INRA, EA2608, CHU-Caen, France

    Serge Carreau

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  1. Khaled Hamden
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  2. Noureddine Allouche
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  3. Bessam Jouadi
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  4. Saloua El-Fazaa
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  6. Serge Carreau
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  7. Mohamed Damak
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  8. Abdelfattah Elfeki
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Correspondence to Khaled Hamden.

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Hamden, K., Allouche, N., Jouadi, B. et al. Inhibitory action of purified hydroxytyrosol from stored olive mill waste on intestinal disaccharidases and lipase activities and pancreatic toxicity in diabetic rats. Food Sci Biotechnol 19, 439–447 (2010). https://doi.org/10.1007/s10068-010-0062-6

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  • DOI: https://doi.org/10.1007/s10068-010-0062-6

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