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Influence of groove depth and surface profile on fluorescence enhancement by grating-coupled surface plasmon resonance

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Abstract

The fluorescence intensity of a sample placed on a metal grating pattern is enhanced due to excitation by the electric field of the grating-coupled surface plasmon resonance (GC-SPR). The dependence of the enhancement on groove depth and surface profile was studied with the aim of improving the sensitivity of fluorescence detection. The enhancement was found to depend on the groove depth, with intensity most enhanced on grating substrate of about 20 nm depth, which produced an intensity about 30 times greater than that on a flat borosilicate glass substrate. Rigorous coupled wave analysis calculation showed that the shape of the groove influenced GC-SPR, suggesting that controlling not only the depth but also the shape of the grating surface profile can be an important factor in improving the sensitivity of detection by fluorescence microscopy.

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Authors and Affiliations

  1. Photonics Research Institute, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, 563-8577, Japan

    Kenji Kintaka & Junji Nishii

  2. Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, 563-8577, Japan

    Hironobu Hori, Keiko Tawa & Yoshiro Tatsu

Authors
  1. Hironobu Hori
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  2. Keiko Tawa
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  3. Kenji Kintaka
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  4. Junji Nishii
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  5. Yoshiro Tatsu
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Correspondence to Keiko Tawa.

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Hori, H., Tawa, K., Kintaka, K. et al. Influence of groove depth and surface profile on fluorescence enhancement by grating-coupled surface plasmon resonance. OPT REV 16, 216–221 (2009). https://doi.org/10.1007/s10043-009-0040-5

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  • DOI: https://doi.org/10.1007/s10043-009-0040-5

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