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The self-assembly, characterization and application of hemoglobin immobilized on Fe3O4@Pt core-shell nanoparticles

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Abstract

Direct electron transfer is demonstrated to occur between an electrode and hemoglobin that was immobilized on a film of Fe3O4@Pt-chitosan (Fe3O4@Pt-CS). Magnetic nanoparticles composed of Fe3O4 were prepared by a chemical coprecipitation method, and platinum nanoparticles were deposited on the Fe3O4 surface to form novel core-shell nanocomposites. In phosphate buffer solution of pH 7.0, the hemoglobin-Fe3O4@Pt-CS assembly on a modified glassy carbon electrode exhibited a couple of well-defined and quasi-reversible redox peaks. The formal potential E0′ was about −0.35 V. The electrode displayed excellent electrocatalytic activity towards oxygen and hydrogen peroxide reduction without the need for an electron mediator.

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Acknowledgement

This work was financially supported by the National Natural Science Foundation of China (Grant numbers: 20675042; 20875051), the Natural Science Foundation of Jiangsu Province (Grant number: BK2009152).

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Authors and Affiliations

  1. Institute of Analytical Chemistry for Life Science, School of Public Health, Nantong University, Nantong, 226007, People’s Republic of China

    Hong Fan, Zhong-Qin Pan & Hai-Ying Gu

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  1. Hong Fan
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  2. Zhong-Qin Pan
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  3. Hai-Ying Gu
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Correspondence to Hai-Ying Gu.

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Fan, H., Pan, ZQ. & Gu, HY. The self-assembly, characterization and application of hemoglobin immobilized on Fe3O4@Pt core-shell nanoparticles. Microchim Acta 168, 239–244 (2010). https://doi.org/10.1007/s00604-009-0279-3

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  • DOI: https://doi.org/10.1007/s00604-009-0279-3

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