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A disposable immunosensor based on gold colloid modified chitosan nanoparticles-entrapped carbon paste electrode

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Abstract

A new strategy is described to construct disposable electrochemical immunosensors for the assay of human immunoglobulin. It is based on a carbon paste electrode constructed from chitosan nanoparticles modified with colloidal gold. The stepwise assembly process of the immunosensor was characterized by means of cyclic voltammetry and electrochemical impedance spectroscopy. Assay conditions that were optimized included the amount of chitosan nanoparticles in the preparation of carbon paste electrode, antibody concentration, and the incubation time of the antibody immobilization. Using hexacyanoferrate as a mediator, the current change increased with the concentration of human immunoglobulin G. A linear relationship in the concentration range 0.3 to 120 ng mL−1 was achieved, with a detection limit of 0.1 ng mL−1 (S/N = 3). The method combines the specificity of the immunological reaction with the sensitivity of the gold colloid amplified electrochemical detection, and it has potential application in clinical immunoassay.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (20805040), Natural Science Foundation of He’nan Province of China (082300420140), Foundation of He’nan Educational Committee (2008A150020) and sponsored by Program for Science & Technology Innovation Talents in Universities of Henan Province (2010HASTIT025).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, 46400, China

    Ke-Jing Huang, Jun-Yong Sun, Chun-Xuan Xu, De-Jun Niu & Wan-Zhen Xie

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  1. Ke-Jing Huang
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  2. Jun-Yong Sun
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  3. Chun-Xuan Xu
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  4. De-Jun Niu
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Correspondence to Ke-Jing Huang.

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Huang, KJ., Sun, JY., Xu, CX. et al. A disposable immunosensor based on gold colloid modified chitosan nanoparticles-entrapped carbon paste electrode. Microchim Acta 168, 51–58 (2010). https://doi.org/10.1007/s00604-009-0254-z

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  • DOI: https://doi.org/10.1007/s00604-009-0254-z

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