Abstract
Objectives: To assess the historical exposure and to study the relationships between lead concentrations in whole blood (B-Pb), plasma (P-Pb), urine (U-Pb), finger bone (Bone-Pb) and duration of employment in workers at a secondary lead smelter and to compare the relationships between B-Pb and P-Pb with results from previous studies of populations with a wide range of lead exposure. Methods: In 39 lead workers (29 active, ten retired), recruited from those with the highest exposure at a German secondary lead smelter, levels of B-Pb, P-Pb and U-Pb were determined by inductively coupled plasma mass spectrometry (ICP-MS). Bone-Pb was determined by in vivo X-ray fluorescence (XRF). Results were compared with data from a previous study on 90 workers (71 active, 19 retired) with lower exposure, from a Swedish secondary lead smelter, as well as with previously collected data from 42 active Russian lead workers and 34 Ecuadorian lead-exposed subjects. Results: The median values in the active/retired German lead workers were: age 44/59 years, duration of employment 20/38 years, Bone-Pb 71/150 μg/g, B-Pb 500/330 μg/l, P-Pb 2.7/1.1 μg/l, and U-Pb 25/13 μmol/mol creatinine. Bone-Pb increased with duration of employment by 4.2 μg/g per year and 1.6 μg/g per year in German and Swedish workers, respectively. The median Bone-Pb was three times higher in both active and retired German workers than in Swedish smelter workers with essentially the same age distribution and duration of employment. The linear regression equation between B-Pb and log P-Pb in the combined group of Ecuadorian, German, Russian and Swedish lead-exposed subjects (n=176) was B-Pb=545×log[P-Pb] + 258 (rs=0.94; P<0.001). Conclusions: The high Bone-Pb values recorded for the German smelters implied a historical lead exposure of considerable magnitude. The long-term high lead exposure also showed up in the B-Pb levels for both active and retired workers, leading to the implementation of necessary industrial safety measures in order to respond to biological threshold limits. The suggested equation describing the relationship between B-Pb and P-Pb in the combined group of subjects with a wide range of lead exposure can be useful in future cross-sectional and longitudinal studies of lead-exposed populations, relating, e.g., lead exposure to adverse health outcomes.
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References
Ahlgren L, Lidén K, Mattsson S, Tejning S (1976) X-ray fluorescence analysis of lead in human skeleton in vivo. Scand J Work Environ Health 2:82–86
Ahlgren L, Haeger-Aronson B, Mattsson S, Schütz A (1980) In vivo determination of lead in the skeleton after occupational exposure to lead. Br J Ind Med 37:109–113
Altman DG (1991) Practical statistics for medical research. Chapman and Hall, London
Barany E, Bergdahl IA, Schütz A, Skerfving S, Oskarsson A (1997) Inductively coupled plasma mass spectrometry for direct multi-element analysis of diluted human blood and serum. J Anal Atom Spectrom 12:1005–1009
Barry PSI (1975) A comparison of concentrations of lead in human tissues. Br J Ind Med 32:119–139
Becker K, Kaus S, Krause C, Lepom P, Schulz C, Seiwert M, Seifert B (2002) German Environmental Survey 1998 (GerES III): environmental pollutants in blood of the German population. Int J Hyg Environ Health. 205:297–308
Bergdahl IA, Desnick RJ, Gerhardsson L, Schütz A, Skerfving S, Wetmur JG (1997a) Delta-aminolevulinic acid dehydratase polymorphism: influence on lead levels and kidney function in humans. Arch Environ Health 52:91–96
Bergdahl IA, Schütz A, Gerhardsson L, Jensen A, Skerfving S (1997b) Lead concentrations in human plasma, urine and whole blood. Scand J Work Environ Health 23:359–363
Bergdahl IA, Sheveleva M, Schütz A, Artamonova VG, Skerfving S (1998) Plasma and blood lead in humans: capacity-limited binding to delta-aminolevulinic acid dehydratase and other lead-binding components. Toxicol Sci 46:247–253
Bergdahl IA, Vahter M, Counter SA, Schütz A, Buchanan LH, Ortega F, Laurell G, Skerfving S (1999) Lead in plasma and whole blood from lead-exposed children. Environ Res 80:25–33
Börjesson J, Mattsson S (2004) X-ray fluorescence in medical sciences. In: Tsuji K, Injuk J, van Grieken R (eds) X-ray spectrometry: recent technological advances. Wiley, England, pp 487–515
Börjesson J, Gerhardsson L, Mattsson S, Schütz A, Skerfving S, Österberg K (1997) In vivo measurements of lead in fingerbone in active and retired lead smelters. Int Arch Occup Environ Health 69:97–105
Brito JA, McNeill FE, Stronach I, Webber CE, Wells S, Richard N, Chettle DR (2001) Longitudinal changes in bone lead concentration: implications for modelling of human bone lead metabolism. J Environ Monit 3:343–351
Christoffersson JO, Schütz A, Ahlgren L, Haeger-Aronsen B, Mattsson S, Skerfving S (1984) Lead in fingerbone analysed in vivo in active and retired lead workers. Am J Ind Med 6:447–457
Christoffersson JO, Schütz A, Skerfving S, Ahlgren L, Mattsson S (1986) Decrease of skeletal lead levels in man after end of occupational exposure. Arch Environ Health 41:312–318
Deutschen Forschungsgemeinschaft (DFG) (2003) Liste aller Änderungen und Neuaufnahmen in der MAK- und BAT-Werte Liste 2000 (http://www.dfg.de)
Ehrlich R, Robins T, Jordaan E, Miller S, Mbuli S, Selby P, Wynchank S, Cantrell A, De Broe M, D’ Haese P, Todd A, Landrigan P (1998) Lead absorption and renal dysfunction in a South African battery factory. Occup Environ Med 55:453–460
European Community (1982) Council directive of 28 July 1982 on the protection of workers from the risk related to exposure to metallic lead and its ionic compounds at work (first individual directive 82/605 EEC). Off J Eur Comm L247:12–21
Gerhardsson L, Attewell R, Chettle DR, Englyst V, Lundström N-G, Nordberg GF, Nyhlin H, Scott MC, Todd AC (1993) In-vivo measurements of lead in bone in long-term exposed lead smelter workers. Arch Environ Health 48:147–156
Lille F, Margules S, Fournier E, Dally S, Garnier R (1994) Effects of occupational lead exposure on motor and somatosensory evoked potentials. Neurotoxicology 15:679–683
Nilsson U, Attewell R, Christoffersson JO, Schütz A, Ahlgren L, Skerfving S, Mattsson S (1991) Kinetics of lead in bone and blood after end of occupational exposure. Pharmacol Toxicol 68:477–484
Sallmen M, Lindbohm ML, Anttila A, Taskinen H, Hemminki K (2000) Time to pregnancy among the wives of men occupationally exposed to lead. Epidemiology 11:141–147
Schütz A, Skerfving S, Christoffersson JO, Ahlgren L, Mattsson S (1987) Lead in vertebral bone biopsies from active and retired lead workers. Arch Environ Health 42:340–346
Schütz A, Bergdahl IA, Ekholm A, Skerfving S (1996) Measurement by ICP-MS of lead in plasma and whole blood of lead workers and controls. Occup Environ Med 53:736–740
Skerfving S (1993) Inorganic lead. In: Beije B, Lundberg P (eds) Criteria documents from the Nordic expert group 1992. National Institute of Occupational Health. Solna, Sweden, pp 125–238
Skerfving S, Nilsson U, Schütz A, Gerhardsson L (1993) Biological monitoring of inorganic lead. Scand J Environ Health 19 [Suppl 1]:59–64
Smith DR, Osterloh JD, Flegal AR (1996) Use of endogenous, stable lead isotopes to determine release of lead from the skeleton. Environ Health Perspect 104:60–66
Somervaille LJ, Nilsson U, Chettle DR, Tell I, Scott MC, Schütz A, Mattsson S, Skerfving S (1989) In vivo measurements of bone lead—a comparison of two X-ray fluorescence techniques used at three different bone sites. Phys Med Biol 34:1833–1845
Acknowledgements
The following organisations and companies are thankfully acknowledged for their help in making this study come true: Staatliches Gewerbeaufsichtsamt Braunschweig (Frau Dr. Bialek and Frau v. Below-Neufeldt), Niedersachsisches Landesamt für Ökologie (Dr. Slupinski), Arztekammer Niedersachsen, BAD Gesundheitsversorge und Sicherheitstechnik GmbH, Bonn (Frau Dr. Schlicker and Herr Müller), TÜV Hannover/Sachsen–Anhalt (Herr Kreienfeld), Philips Medizin Systeme, Hamburg and Herr Notte at the German lead smelter plant. This paper is dedicated to the memory of Andrejs Schütz, Ph.D., who sadly passed away in the summer of 2001.
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Schütz, A., Olsson, M., Jensen, A. et al. Lead in finger bone, whole blood, plasma and urine in lead-smelter workers: extended exposure range. Int Arch Occup Environ Health 78, 35–43 (2005). https://doi.org/10.1007/s00420-004-0559-5
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DOI: https://doi.org/10.1007/s00420-004-0559-5