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Induction of micronuclei in human fibroblasts and keratinocytes by 25 kV x-rays

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Abstract

A relative biological effectiveness (RBE) not much larger than unity is usually assumed for soft x-rays (up to ≈50 keV) that are applied in diagnostic radiology such as mammography, in conventional radiotherapy and in novel radiotherapy approaches such as x-ray phototherapy. On the other hand, there have been recent claims of an RBE of more than 3 for mammography and respective conventional x-rays. Detailed data on the RBE of soft x-rays, however, are scarce. The aim of the present study was to determine the effect of low-energy x-rays on chromosomal damage in vitro, interms of micronucleus induction. Experiments were performed with 25 kV x-rays and a 200 kV x-ray reference source. The studies were carried out on primary human epidermal keratinocytes (HEKn), human fibroblasts (HFIB) and NIH/3T3 mouse fibroblasts. Micronucleus (MN) induction was assayed after in vitro irradiation with doses ranging from 1 to 5.2 Gy. Compared to the effect of 200 kV x-rays, 25 kV x-rays resulted in moderately increased chromosomal damage in all cell lines studied. This increase was observed for the percentage of binucleated (BN) cells with micronuclei as well as for the number of micronuclei per BN cell. Moreover, the increased number of micronuclei per micronucleated BN cell in human keratinocytes and 3T3 mouse fibroblasts suggests that soft x-rays induce a different quality of damage. For all cell lines studied the analysis of micronucleus induction by 25 kV soft x-rays compared to 200 kV x-rays resulted in an RBE value of about 1.3. This indicates a somewhat enhanced potential of soft x-rays for induction of genetic effects.

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References

  1. Brenner DJ, Amols HI (1989) Enhanced risk from low-energy screen-film mammography X rays. Br J Radiol 62:910–914

    Article  Google Scholar 

  2. Kellerer AM (2002) Electron spectra and the RBE of X rays. Radiat Res 158:13–22

    Article  Google Scholar 

  3. Bistrović M, Bišćan M, Viculin T (1986) RBE of 20 kV x-rays determined for survival of V79 cells. Radiother Oncol 7:175–180

    Article  Google Scholar 

  4. Hoshi M, Antoku S, Nakamura N, Russel WJ, Miller RC, Sawada S, Mizuno M, Nishio S (1988) Soft x-rays dosimetry and RBE for survival of Chinese hamster V79 cells. Int J Radiat Biol 54:577–591

    Article  Google Scholar 

  5. Spadinger I, Palcic B (1992) The relative biological effectiveness of 60Co γ-rays, 55 kVp x-rays, 250 kVp x-rays, and 11 MeV electrons at low doses. Int J Radiat Biol 61:345–353

    Article  Google Scholar 

  6. Frankenberg D, Kelnhofer K, Bär K, Frankenberg-Schwager M (2002) Enhanced neoplastic transformation by mammography X rays relative to 200 kVp X rays: indication for a strong dependence on photon energy of the RBEm for various end points. Radiat Res 157:99–105. Erratum in: Radiat Res 158:126

    Article  Google Scholar 

  7. Virsik RP, Harder D, Hansmann I (1977) The RBE of 30 kV x-rays for the induction of dicentric chromosomes in human lymphocytes. Radiat Environ Biophys 14:109–121

    Article  Google Scholar 

  8. Virsik RP, Harder D (1981) Analysis of radiation-induced acentric fragments in human G0 lymphocytes. Radiat Environ Biophys 19:29–40

    Article  Google Scholar 

  9. Sasaki MS, Kobayashi K, Hieda K, Yamada T, Ejima Y, Maezawa H, Furusawa Y, Ito T, Okada S (1989) Induction of chromosome aberrations in human lymphocytes by monochromatic x-rays of quantum energy between 4.8 and 14.6 keV. Int J Radiat Biol 56:975–988

    Article  Google Scholar 

  10. Frankenberg-Schwager M, Garg I, Frankenberg D, Greve B, Severin E, Uthe D, Göhde W (2002) Mutagenicity of low-filtered 30 kVp x-rays, mammography x-rays and conventional x-rays in cultured mammalian cells. Int J Radiat Biol 78:781–789

    Article  Google Scholar 

  11. Fenech M, Morley AA (1985) Measurement of micronuclei in lymphocytes. Mutat Res 147:29–36

    Google Scholar 

  12. Fenech M, Morley AA (1986) Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo ageing and low dose X-irradiation. Mutat Res 161:193–198

    Google Scholar 

  13. Littlefield LG, Sayer AM, Frome EL (1989) Comparison of dose-response parameters for radiation-induced acentric fragments and micronuclei observed in cytokinesis-arrested lymphocytes. Mutagenesis 4:265–270

    Article  Google Scholar 

  14. Thierens H, Vral A, De Ridder L (1991) Biological dosimetry using the micronucleus assay for lymphocytes; interindividual differences in dose-response. Health Physics 61:623–630

    Article  Google Scholar 

  15. Verhaegen F, Vral A (1994) Sensitivity of micronucleus induction in human lymphocytes to low-LET radiation qualities: RBE and correlation of RBE and LET. Radiat Res 139:208–213

    Article  Google Scholar 

  16. Vral A, Verhaegen F, Thierens H, De Ridder L (1994) Micronuclei induced by fast neutrons versus 60Co γ-rays in human peripheral blood lymphocytes. Int J Radiat Biol 65:321–328

    Article  Google Scholar 

  17. Słonina D, Gasińska A (1997) Intrinsic radiosensitivity of healthy donors and cancer patients as determined by the lymphocytes micronucleus assay. Int J Radiat Biol 72:693–701

    Article  Google Scholar 

  18. Słonina D, Klimek M, Szpytma T, Gasińska A (2000) Comparison of the radiosensitivity of normal-tissue cells with normal-tissue reactions after radiotherapy. Int J Radiat Biol 76:1255–1264

    Article  Google Scholar 

  19. Mill AJ, Wells J, Hall SC, Butler A (1996) Micronucleus induction in human lymphocytes: comparative effects of x-rays, alpha particles, beta particles and neutrons and implications for biological dosimetry. Radiat Res 145:575–585

    Article  Google Scholar 

  20. Boone JM, Seibert JA (1997) An accurate method for computer-generating tungsten anode x-ray spectra from 30 to 140 kV. Med Phys 24:1661–1670

    Article  Google Scholar 

  21. StatSoft Inc. (1996) STATISTICA for Windows (Computer program manual). Tulsa, OK, USA

  22. Goddard AD, Heddle JA, Gallie BL, Philips RA (1985) Radiation sensitivity of fibroblasts of bilateral retinoblastoma patients as determined by micronucleus induction in vitro. Mutat Res 152:31–38

    Google Scholar 

  23. Johansen J, Streffer C, Fuhrmann C, Bentzen SM, Stausbol-Gron B, Overgaard M, Overgaard J (1998) Radiosensitivity of normal fibroblasts from breast cancer patients assessed by the micronucleus and colony assays. Int J Radiat Biol 73:671–678

    Article  Google Scholar 

  24. Hall SC, Mill AJ, Allen LA, Butler A, Crowe MH, Hart CD (1991) Radiological-protection-related neutron studies using the C3H 10T1/2 cell system: I. The RBE of 24 keV neutrons; II. An investigation into the inverse dose-rate effect using 2.5 MeV neutrons. In: Symour CB, Mothersill C (eds) New developments in fundamental and applied radiobiology. Taylor & Francis, London New York Philadelphia, pp 202–207

    Google Scholar 

  25. Edwards AA, Lloyd DC, Purrot RJ (1979) Radiation induced chromosome aberrations and the Poisson distribution. Radiat Environ Biophys 16:89–100

    Article  Google Scholar 

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Author notes

  1. Dorota Słonina

    Present address: Klinik und Poliklinik für Strahlentherapie und Radioonkologie, Medizinische Fakultät Carl Gustav Carus der Technischen Universität Dresden, Fetscherstrasse 74, PF 58, 01307, Dresden, Germany

Authors and Affiliations

  1. Laboratory of Radiation Biology, Centre of Oncology, Kraków, Poland

    Dorota Słonina

  2. Klinik und Poliklinik für Strahlentherapie und Radioonkologie, Medizinische Fakultät Carl Gustav Carus der Technischen Universität Dresden, Fetscherstrasse 74, PF 58, 01307, Dresden, Germany

    Kathrin Spekl, Katarina Brankovic, Cordelia Hoinkis & Wolfgang Dörr

  3. Forschungszentrum Rossendorf, Dresden, Germany

    Anna Panteleeva

Authors
  1. Dorota Słonina
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  2. Kathrin Spekl
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  3. Anna Panteleeva
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  4. Katarina Brankovic
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  5. Cordelia Hoinkis
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  6. Wolfgang Dörr
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Corresponding author

Correspondence to Wolfgang Dörr.

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Published online: 25 February 2003

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Słonina, D., Spekl, K., Panteleeva, A. et al. Induction of micronuclei in human fibroblasts and keratinocytes by 25 kV x-rays. Radiat Environ Biophys 42, 55–61 (2003). https://doi.org/10.1007/s00411-003-0177-8

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  • DOI: https://doi.org/10.1007/s00411-003-0177-8

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