Abstract
Highly concentrated oil-in-water (o/w) emulsion stabilised by means of gluten and soya protein isolate (SPI) at low pH have been characterized by means of linear dynamic viscoelasticity and droplet size distribution analysis (DSD). The microstructure of these emulsions has been characterized at a colloidal level by using confocal laser scanning microscopy (CLSM) and light microscopy (LM). These emulsions always exhibited a behaviour characteristic of highly flocculated emulsions with a mechanical spectrum showing a well-developed plateau region. DSD results generally showed log normal bimodal profiles. Microstructure images revealed occurrence of a close packing of droplets with a broad distribution of sizes participating in the formation of a three dimensional flocculated network. The Mason model of elasticity of compressed emulsions has been used to correlate viscoelastic and microstructural parameters giving adequate fitting but underestimating the elastic properties obtained for the highest concentration of gluten. These deviations may be explained in terms of an enhancement of the elastic network formed in the aqueous phase in which the glutenin fraction must play an important role.
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Arfvidsson C, Wahlund KG, Eliasson AC (2004) Direct molecular weight determination in the evaluation of dissolution methods for unreduced glutenin. J Cereal Sci 39(1):1–8
Bressy L, Hébraud P, Schmitt V, Bibette J (2003) Rheology of emulsions stabilized by solid interfaces. Langmuir 19:598–604
Bugusu B, Rajwa B, Hamacker BH (2002) Interaction of maize zein with wheat gluten in composite dough and bread as determined by confocal laser scanning microscopy. Scanning 24:1–5
Carceller JL, Aussenac T (2002) Size characterization of glutenin polymers by HPSEC-MALLS. J Cereal Sci 33(2):131–142
Dickinson E (2001) Milk protein interfacial and the relationship to emulsion stability and rheology. Colloids Surf B: Biointerfaces 20:197–210
Dimitrova TD, Leal-Calderón F (2001) Bulk elasticity of concentrated protein-stabilized emulsions. Langmuir 17:3235–3244
Dimitrova TD, Leal-Calderón F (2004) Rheological properties of highly concentrated protein-stabilized emulsions. Adv Colloid Interface Sci 108–109:49–61
Elizalde BE, Bartholomai GB, Pilosof AMR (1996) The effect of pH on the relationship between hydrophilic/lipophilic characteristics and emulsification properties of soy proteins. Lebensm Wiss Technol 29:334–339
Franco JM, Guerrero A, Gallegos C (1995) Rheology and processing of salad dressing emulsions. Rheol Acta 34:513–524
Fukushima D (1991) Structures of plant storage proteins and their functions. Food Rev Int 7(3):353–381
Gallegos C, Franco JM, Madiedo JM, Raymundo A, Sousa I (2002) In: Welti-Chanes J, Barbosa-Cánovas GV, Aguilera JM (eds) Engineering and food for the 21st century. CRC, Boca Raton
Guerrero A, Partal P, Gallegos C (1998) Linear viscoelastic properties of sucrose ester stabilized oil-in-water emulsions. J Rheol 42:1375–1388
Hoseney RC, Rogers DE (1990) The formation and properties of wheat flour doughs. CRC Crit Rev Food Sci Nutr 29(2):73–93
Kalichevsky MT, Jaroszkiewicz EM, Blanshard JMV (1992) Glass transition of gluten 2: the effects of lipids and emulsifiers. Int J Biol Macromol 1:267–273
Langton M, Jordansson E, Altskär A, Sorensen C, Hermansson A-M (1999) Microstructure and image analysis of mayonnaises. Food Hydrocoll 13:113–125
Mason TG, Lacasse MD, Levine D, Grest GS, Bibette J, Weiltz DA (1997) Osmotic pressure and viscoelastic shear moduli of monodisperse emulsions. Phys Rev 56:3150–3166
Morales A, Kokini JL (1997) Glass transition of soy globulins using DSC and mechanical spectrometry. Biotechnol Prog 13:624–629
Partal P, Guerrero A, Berjano M, Gallegos C (1997) Influence of concentration and temperature on the flow behaviour of O/W emulsions stabilized by a sucrose palmitate. J Am Oil Chem Soc 74:1203–1212
Perrin P (2000) Droplet–droplet interactions in both direct and inverse emulsions stabilized by a balanced amphiphilic polyelectrolyte. Langmuir 16:881–884
Pons R, Solans C, Tadros ThF (1995) Rheological behavior of highly concentrated oil-in-water (o/w) emulsions. Langmuir 11:1966–1971
Princen HM (1983) Rheology of foams and highly concentrated emulsions. J Colloid Interface Sci 91(1):160–175
Princen HM, Kiss AD (1986) Rheology of foams and highly concentrated emulsions. J Colloid Interface Sci 112:427–437
Sánchez MC, Berjano M, Brito E, Guerrero A, Gallegos C (1998) Evolution of the microstructure and rheology of o/w emulsions during the emulsification process. Can J Chem Eng 76:479–485
Sánchez MC, Berjano M, Guerrero A, Gallegos C (2001) Emulsification rheokinetics of nonionic surfactant-stabilized oil-in-water emulsions. Langmuir 17:5410–5416
Shewry PR, Tatham AS, Forde J, Kreis M, Miflin BJ (1986) The classification and nomenclature of wheat gluten proteins: a reassessment. J Cereal Sci 4:97–106
Tornberg E (1978) Functional characteristics of protein stabilized emulsions: emulsifying behaviour of proteins in a valve homogenizer. J Food Sci 29:867–879
Wu S (1989) Chain structure and entanglement. J Polym Sci 27:723–741
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This work was part of a research project No. AGL2002-01106, supported by the Spanish MCYT. The financial support is gratefully acknowledged.
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Paper presented at the Annual European Rheology Conference (AERC) 2005, April 21-23, Grenoble, France.
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Bengoechea, C., Cordobés, F. & Guerrero, A. Rheology and microstructure of gluten and soya-based o/w emulsions. Rheol Acta 46, 13–21 (2006). https://doi.org/10.1007/s00397-006-0102-6
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DOI: https://doi.org/10.1007/s00397-006-0102-6