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Novel core-shell lipid-chitosan and lipid-poloxamer nanocapsules: stability by hydration forces

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Abstract

In the last few years, a great attention has been paid to core-shell structured lipid nanocapsules. The interest of these colloids lies in their very promising applications as delivery systems of hydrophobic drugs or as DNA carriers. The aim of this work was to provide fuller knowledge concerning the physicochemical properties of these new colloidal particles, paying special attention to the role played by the constituting components. Our lipid nanocapsules were comprised of a triglyceride-lecithin core surrounded by a chitosan and/or poloxamer (Pluronic® F68) shell. Four different systems were formulated by varying the chitosan and poloxamer contents. We have estimated their shell composition from electrokinetic mobility measurements and stability studies. They revealed a high incorporation of chitosan to the shell, while Pluronic® F68 only presented a secondary role during the nanocapsule formation, providing final systems with low poloxamer content. In addition, the stability studies, performed not only on ideal solutions but also on simulated physiological fluids, revealed that hydration forces were crucial to maintain the integrity of these nanocapsules under physicochemical conditions similar to those found in real physiological fluids. Theoretical treatment of stability data has allowed us to determine the role that ions play on hydration forces when they act as counterions.

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References

  1. Drummond DC, Meyer O, Hong K, Kirpotin DB, Papahadjopoulos D (1999) Pharmacol Rev 51:691

    CAS  Google Scholar 

  2. Brigger I, Dubernet C, Couvreur P (2002) Adv Drug Deliv Rev 54:631

    Article  CAS  Google Scholar 

  3. Jones MC, Leroux JC (1999) Eur J Pharm Biopharm 48:101

    Article  CAS  Google Scholar 

  4. Bae KH, Lee Y, Park TG (2007) Biomacromolecules 8:650

    Article  CAS  Google Scholar 

  5. Lambert G, Fattal E, Pinto-Alphandary H, Gulik A, Couvreur P (2000) Pharm Res 17:707

    Article  CAS  Google Scholar 

  6. Khalid MN, Simard P, Hoarau D, Dragomir A, Leroux JC (2006) Pharm Res 23:752

    Article  CAS  Google Scholar 

  7. Prego C, Torres D, Fernandez-Megia E, Novoa-Carballal R, Quiñoá E, Alonso MJ (2006) J Control Release 111:299

    Article  CAS  Google Scholar 

  8. Lee SH, Choi SH, Kim SH, Park TG (2008) J Control Release 125:25

    Article  CAS  Google Scholar 

  9. Rübe A (2006) Development and physico-chemical characterization of nanocapsules. Thesis, Martin-Luther-University, Germany

  10. Gabizon A, Catane R, Uziely B, Kaufman B, Safra T, Cohen R, Martín F, Huang A, Barenholz Y (1994) Cancer Res 54:987

    CAS  Google Scholar 

  11. Tobio M, Sánchez A, Vila A, Soriano I, Evora C, Vila-Jato JL, Alonso MJ (2000) Colloids Surf B 18:315

    Article  CAS  Google Scholar 

  12. Moghimi SM, Hunter AC (2001) Crit Rev Ther Drug Carr Syst 18:527

    CAS  Google Scholar 

  13. Barrat G (2003) Cell Mol Life Sci 60:21

    Article  Google Scholar 

  14. Desai MP, Labhasetwar V, Walter E, Levy RJ, Amidon GL (1997) Pharm Res 14:1568

    Article  CAS  Google Scholar 

  15. Calvo P, Remuñán-López C, Vila-Jato JL, Alonso MJ (1997) Colloid Polym Sci 275:46

    Article  CAS  Google Scholar 

  16. Fessi H, Puisieux F, Devissaguet JP, Ammoury N, Benita S (1989) Int J Pharm 55:25

    Article  Google Scholar 

  17. Prego CT, Torres D, Alonso MJ (2006) J Drug Del Sci Tech 16:331

    CAS  Google Scholar 

  18. Lozano MV, Torrecilla D, Torres D, Vidal A, Dominguez F, Alonso MJ (2008) Biomacromolecules 9:2186

    Article  CAS  Google Scholar 

  19. Hiemenz PC, Rajagopalan R (1997) Principles of colloidal and surface chemistry. Dekker, New York

    Google Scholar 

  20. Overbeek JTG (1982) Adv Colloid Interface Sci 16:17

    Article  CAS  Google Scholar 

  21. García-Fuentes M, Prego C, Torres D, Alonso MJ (2004) Bull Tech Gattefossé 97:51

    Google Scholar 

  22. Olbrich C, Müller RH (1999) Int J Pharm 180:31

    Article  CAS  Google Scholar 

  23. López-León T, Santander-Ortega M, Ortega-Vinuesa JL, Bastos-González D (2008) J Phys Chem C 112:16060

    Article  Google Scholar 

  24. Molina-Bolivar JA, Galisteo-Gonzalez F, Quesada-Perez M, Hidalgo-Alvarez R (1998) Colloid Polym Sci 276:1117

    Article  CAS  Google Scholar 

  25. Molina-Bolivar JA, Ortega-Vinuesa JL (1999) Langmuir 15:2644

    Article  CAS  Google Scholar 

  26. Santander-Ortega MJ, Bastos-Gonzalez D, Ortega-Vinuesa JL (2007) Colloids Surf B Biointerfaces 60:80

    Article  CAS  Google Scholar 

  27. Molina-Bolivar JA, Galisteo-Gonzalez F, Hidalgo-Alvarez R (2000) Spanish patent office ES2141684

  28. Quintanar-Guerrero D, Allémann E, Fessi H, Doelker E (1998) Drug Dev Ind Pharm 24:1113

    Article  CAS  Google Scholar 

  29. Prego C, Fabre M, Torres D, Alonso MJ (2006) Pharm Res 23:549

    Article  CAS  Google Scholar 

  30. Soppimath KS, Aminabhavi TM, Kulkarni AR, Rudzinski WE (2001) J Control Release 70:1

    Article  CAS  Google Scholar 

  31. Letchford K, Burt H (2007) Eur J Pharm Biopharm 65:259

    Article  CAS  Google Scholar 

  32. Singer JM, Vekermans FCA, Lichtenbelt JWTh, Hesselkink Th, Wiersema APH (1973) J Colloid Interface Sci 45:608

    Article  CAS  Google Scholar 

  33. Quesada M, Puig J, Delgado JM, Hidalgo-Álvarez R (1998) J Biomater Sci Polymer Ed 9:961

    Article  CAS  Google Scholar 

  34. Pashley RM (1982) Adv Colloid Interface Sci 16:57

    Article  CAS  Google Scholar 

  35. Churaev NV, Derjaguin BV (1985) J Colloid Interface Sci 103:542

    Article  CAS  Google Scholar 

  36. Israelachvili JN (1992) Intermolecular and surface forces. Academic, London

    Google Scholar 

  37. Matijevic E, Mathai KG, Ottewill RH, Kerker M (1961) J Phys Chem 65:826

    Article  CAS  Google Scholar 

  38. Fuchs N (1934) Z Phy. 89:736

    Article  Google Scholar 

  39. Honing EP, Roebersen GI, Wiersema PH (1985) J Colloid Interface Sci 104:553

    Article  Google Scholar 

  40. Papenhuijen J, Fleer GJ, Bijsterbosch BHJ (1985) J Colloid Interface Sci 104:553

    Article  Google Scholar 

  41. Peula JM, Fernandez-Barbero A, Hidalgo-Alvarez R, de las Nieves FJ (1997) Langmuir 13:3938

    Article  CAS  Google Scholar 

  42. Israelachvili J (1991) Intermolecular and surface forces, 2nd edn. Academic, New York

    Google Scholar 

  43. Allen TM, Austin GA, Chonn A, Lin L, Lee KC (1991) Biochim Biophys Acta 1061:56

    Article  CAS  Google Scholar 

  44. Florence AT (1997) Pharm Res 14:259

    Article  CAS  Google Scholar 

  45. Santander-Ortega MJ, Jódar-Reyes AB, Csaba N, Bastos-González D, Ortega-Vinuesa JL (2006) J Colloid Interface Sci 302:522

    Article  CAS  Google Scholar 

  46. López-León T, Carvalho ELS, Seijo B, Ortega-Vinuesa JL, Bastos-González D (2005) J Colloid Interface Sci 283:344

    Article  Google Scholar 

  47. Sonvico F, Cagnani A, Rossi A, Motta S, Di Bari MT, Cavatorta F, Alonso MJ, Deriu A, Colombo P (2006) Int J Pharm 324:67

    Article  CAS  Google Scholar 

  48. Schatz C, Pichot C, Delair T, Viton C, Domard A (2003) Langmuir 19:9896

    Article  CAS  Google Scholar 

  49. Calvo P, Remuñán-López C, Vila-Jato JL, Alonso MJ (1997) J Appl Polym Sci 63:125

    Article  CAS  Google Scholar 

  50. Janes KA, Fresneau MP, Marazuela A, Fabra A, Alonso J (2001) J Control Release 73:255

    Article  CAS  Google Scholar 

  51. Vila A, Sánchez A, Tobío M, Calvo P, Alonso MJ (2002) J Control Release 78:15

    Article  CAS  Google Scholar 

  52. Berthold A, Kremer K, Kreuter J (1996) J Control Release 39:17

    Article  CAS  Google Scholar 

  53. van der Lubben IM, Verhoef JC, Van Aelst AC, Borchard G, Junginger HE (2001) Biomaterials 22:687

    Article  Google Scholar 

  54. Santander-Ortega MJ, Csaba N, Alonso MJ, Ortega-Vinuesa JL, Bastos-González D (2007) Colloids Surf A 296:132

    Article  CAS  Google Scholar 

  55. Calvo P, Vila-Jato JL, Alonso MJ (1997) Int J Pharm 153:41

    Article  CAS  Google Scholar 

  56. Mosqueira VCF, Philippe L, Barratt G (2006) J Nanosci Nanotechnol 6:3193

    Article  CAS  Google Scholar 

  57. Thompson DW, Collins IR (1994) J Colloid Interface Sci 163:347

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to express their appreciation for the financial support granted by the “Ministerio de Ciencia e Innovación” (MICINN, Spain), projects MAT2007-66662-C02-01 and MAT2006-13646-C03-03 (European FEDER support included), and from the “Conserjería de Innovación, Ciencia y Tecnología de la Junta de Andalucía” (Spain), projects of excellence P07-FQM2496 and P07-FQM3099. Moreover, M.V. Lozano is grateful for the fellowship received from the Spanish Government (AP2005-1701). Finally, we wish to thank Professor M.J. Alonso for her supervision.

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Authors and Affiliations

  1. Biocolloid and Fluid Physics Group, Department of Applied Physics, University of Granada, 18071, Granada, Spain

    Manuel J. Santander-Ortega, Delfina Bastos-González & Juan L. Ortega-Vinuesa

  2. Department of Pharmacy and Pharmaceutical Technology, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain

    María V. Lozano-López

  3. Department of Applied Physics, University of Málaga, 29071, Málaga, Spain

    José M. Peula-García

Authors
  1. Manuel J. Santander-Ortega
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  2. María V. Lozano-López
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  3. Delfina Bastos-González
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  4. José M. Peula-García
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  5. Juan L. Ortega-Vinuesa
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Correspondence to Juan L. Ortega-Vinuesa.

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Santander-Ortega, M.J., Lozano-López, M.V., Bastos-González, D. et al. Novel core-shell lipid-chitosan and lipid-poloxamer nanocapsules: stability by hydration forces. Colloid Polym Sci 288, 159–172 (2010). https://doi.org/10.1007/s00396-009-2132-y

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  • DOI: https://doi.org/10.1007/s00396-009-2132-y

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