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Protein-loaded PLGA–PEO blend nanoparticles: encapsulation, release and degradation characteristics

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Abstract

The aim of this work was to study the variables that affect the encapsulation and release of proteins from nanoparticles based on poly(lactic-co-glycolic acid; PLGA)–poloxamer and PLGA–poloxamine blend matrices, using bovine serum albumin (BSA) and immuno-γ-globulin (IgG) as model proteins. The nanoparticles were prepared by a solvent diffusion technique, and the studied variables were (1) PLGA molecular weight, (2) type of PEO-block copolymers, (3) protein loading, (4) pH and, (5) volume of the protein solution. Our results showed that the proteins can be efficiently incorporated into and released from the blend matrices. The type of the PEO derivative and the pH of the internal aqueous phase were the most important factors influencing protein encapsulation and release kinetics. Moreover, comparative degradation study of PLGA, PLGA–poloxamer and PLGA–poloxamine nanoparticles confirmed that the degradation and release characteristics of polyester particles can be improved by the incorporation of polyoxyethylene derivatives with different hydrophilia–lipophilia balance.

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Acknowledgments

Authors thank the financial support from the "Comisión Interministerial de Ciencia y Tecnología" projects MAT2006-13646-C03-03 and MAT2007-66662-C02-01 (European FEDER support included) and from the "Consejería de Innovación, Ciencia y Tecnología de la Junta de Andalucía" Projects P07-FQM-2496 and P07-FQM03099.

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Authors and Affiliations

  1. Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain

    Manuel J. Santander-Ortega, Noemi Csaba, Lisette González & Maria J. Alonso

  2. Biocolloid and Fluid Physics Group, Department of Applied Physics, University of Granada, 18071, Granada, Spain

    Manuel J. Santander-Ortega, Delfina Bastos-González & Juan L. Ortega-Vinuesa

Authors
  1. Manuel J. Santander-Ortega
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  2. Noemi Csaba
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  3. Lisette González
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  4. Delfina Bastos-González
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  5. Juan L. Ortega-Vinuesa
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  6. Maria J. Alonso
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Corresponding author

Correspondence to Maria J. Alonso.

Additional information

Manuel J. Santander-Ortega and Noemi Csaba have equally contributed to the present work.

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Santander-Ortega, M.J., Csaba, N., González, L. et al. Protein-loaded PLGA–PEO blend nanoparticles: encapsulation, release and degradation characteristics. Colloid Polym Sci 288, 141–150 (2010). https://doi.org/10.1007/s00396-009-2131-z

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  • DOI: https://doi.org/10.1007/s00396-009-2131-z

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