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Immobilization of avidin on (001) GaAs surface

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Abstract

To reliably immobilize different biomoieties on surfaces of III-V semiconductors is one of the most critical issues in the development of biodetector devices based on the optical/electronic properties of these materials. Herein we demonstrate the successful immobilization of avidin, a robust and well-studied protein, on a (001) GaAs surface. The immobilization was investigated via specific binding to biotin, which was connected to the GaAs surface through commercially available long- or short-chain amino group terminated alkanethiols (HS(CH2)11NH2 or HS(CH2)2NH2), or through a biotinylated thiol synthesized in our laboratory. The immobilization performance was evaluated by photoluminescence and fluorescence microscopy measurements. We found that the biotinylated thiol mixed with a diluent thiol provides the highest avidin immobilization efficiency.

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Authors and Affiliations

  1. Département de Génie Électrique et de Génie Informatique, Université de Sherbrooke, J1K 2R1, Sherbrooke, Québec, Canada

    X. Ding, Kh. Moumanis & J.J. Dubowski

  2. Département de Microbiologie et d’Infectiologie, Université de Sherbrooke, J1H 5N4, Sherbrooke, Québec, Canada

    E.H. Frost

  3. Département de Pharmacologie, Université de Sherbrooke, J1H 5N4, Sherbrooke, Québec, Canada

    E. Escher

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  1. X. Ding
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  2. Kh. Moumanis
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  3. J.J. Dubowski
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  4. E.H. Frost
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  5. E. Escher
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Corresponding author

Correspondence to J.J. Dubowski.

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PACS

81.05.Ea; 82.65.+r; 87.14.Ee

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Ding, X., Moumanis, K., Dubowski, J. et al. Immobilization of avidin on (001) GaAs surface. Appl. Phys. A 83, 357–360 (2006). https://doi.org/10.1007/s00339-006-3569-1

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  • DOI: https://doi.org/10.1007/s00339-006-3569-1

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