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Enzyme-catalyzed peptide syntheses in aqueous polymer two-phase systems

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Summary

Peptide synthesis in the aqueous polymer two-phase system was examined. Water soluble polymers adopted were dextran and polyethyleneglycol (PEG). By the modification of a proteolytic enzyme, trypsin, with dextran carrying a very small number of carboxyl groups, a separation of the enzyme from its peptide product, N-benzoyl-L-arginine glycinamide, which is relatively more soluble in a PEG phase could be realized. This separation drastically reduced a so-called "mass-law" effect, and increased the yield of the reaction product. The aqueous polymer two-phase system would be useful to enhance the usefulness of biocatalysts in organic syntheses.

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Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, Toyama University, 930, Toyama, Japan

    Yasushi Maeda, Hitomi Ito, Rie Izumida & Hiromi Kitano

Authors
  1. Yasushi Maeda
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  2. Hitomi Ito
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  3. Rie Izumida
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  4. Hiromi Kitano
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Maeda, Y., Ito, H., Izumida, R. et al. Enzyme-catalyzed peptide syntheses in aqueous polymer two-phase systems. Polymer Bulletin 38, 49–53 (1997). https://doi.org/10.1007/s002890050018

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  • DOI: https://doi.org/10.1007/s002890050018

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