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Needle-type glucose microbiosensor based on glucose oxidase immobilised in an overoxidised polypyrrole film (an in-vitro study)

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Abstract

A fast response, needle-type glucose microbiosensor has been fabricated by a one-step electrochemical immobilisation of glucose oxidase in a polypyrrole film. The sensor shows a remarkable rejection of electroactive interferences, especially paracetamol. The maximum bias observed in the worst situation never exceeded the value of 6%. The fabrication procedure delivered very reproducible devices and the sensitivity of a newly prepared biosensor was typically 650 nAmM–1cm–2. The kinetic parameters, obtained from an existing model, permitted to understand the sensor behaviour.

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Authors and Affiliations

  1. Dipartimento di Chimica, Università degli Studi di Bari, Via E. Orabona, 4–70126 Bari, Italy e-mail: palmisano@chimica.uniba.it, , , , , , IT

    M. Quinto, I. Losito & F. Palmisano

  2. Dipartimento di Chimica, Università degli Studi della Basilicata, Via N. Sauro, 85–85100 Potenza, Italy, , , , , , IT

    C. G. Zambonin

Authors
  1. M. Quinto
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  2. I. Losito
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  3. F. Palmisano
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  4. C. G. Zambonin
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Received: 4 February 2000 / Revised: 2 May 2000 / Accepted: 6 May 2000

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Quinto, M., Losito, I., Palmisano, F. et al. Needle-type glucose microbiosensor based on glucose oxidase immobilised in an overoxidised polypyrrole film (an in-vitro study). Fresenius J Anal Chem 367, 692–696 (2000). https://doi.org/10.1007/s002160000489

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  • DOI: https://doi.org/10.1007/s002160000489

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