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Electrogeneration of polyluminol and chemiluminescence for new disposable reagentless optical sensors

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Abstract

A performant reagentless electrochemiluminescent (ECL) detection system for H2O2 is presented, based on an electropolymerized polyluminol film prepared under near-neutral conditions. Such an original polyluminol electrodeposition is reported for the first time and on a screen-printed electrode (SPE) surface. Electropolymerized luminol acts as an active luminophore of the electrochemiluminescent reaction, as the monomer does. Polymerization conditions have been optimized in order to obtain the best ECL responses to H2O2. By performing electrodeposition in a potentiostatic mode, at 425 mV vs. Ag|AgCl, in 0.1 mol L−1 phosphate/0.1 mol L−1 KCl pH 6 and 1 mmol L−1 luminol, with a total charge of 0.5 mC, the linear range for H2O2 detection extends from 7.9 × 10−8 mol L−1 to 1.3 × 10−3 mol L−1. Such performant disposable reagentless easy-to-use miniaturized systems based on SPEs should be applicable to the electrochemiluminescent detection of many oxidase-substrate compounds.

An original polyluminol electrodeposition process on a screen-printed electrode surface is reported for the first time. The polymeric structure is demonstrated to behave as an electrochemiluminescent luminophore, allowing disposable reagentless easy-to-use optical sensors for hydrogen peroxide detection to be designed.

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Authors and Affiliations

  1. Université de Lyon, Université Lyon 1, CNRS, UMR5246, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Laboratoire de Génie Enzymatique et Biomoléculaire, F-69622, Villeurbanne, Cedex, France

    Audrey Sassolas, Loïc J. Blum & Béatrice D. Leca-Bouvier

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  1. Audrey Sassolas
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  2. Loïc J. Blum
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  3. Béatrice D. Leca-Bouvier
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Correspondence to Béatrice D. Leca-Bouvier.

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Sassolas, A., Blum, L.J. & Leca-Bouvier, B.D. Electrogeneration of polyluminol and chemiluminescence for new disposable reagentless optical sensors. Anal Bioanal Chem 390, 865–871 (2008). https://doi.org/10.1007/s00216-007-1731-z

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  • DOI: https://doi.org/10.1007/s00216-007-1731-z

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