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Within the cell: analytical techniques for subcellular analysis

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Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

This review covers recent developments in the preparation, manipulation, and analyses of subcellular environments. In particular, it highlights approaches for (1) separation and detection of individual organelles, (2) preparation of ultra-pure organelle fractions, and (3) utilization of novel labeling strategies. These approaches, based on innovative technologies such as microfluidics, immunoisolation, mass spectrometry and electrophoresis, suggest that subcellular analyses will soon become as commonplace as single cell and bulk cellular assays.

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Fig. 1
Fig. 2a–c
Fig. 3a–f
Fig. 4a–d
Fig. 5a–d

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Abbreviations

AFM:

Atomic force microscopy

ATP:

Adenosine triphosphate

BSA:

Bovine serum albumin

CE:

Capillary electrophoresis

ECFP:

Enhanced cyan fluorescent protein

ER:

Endoplasmic reticulum

EYFP:

Enhanced yellow fluorescent protein

FACS:

Fluorescence-activated cell sorting

FFE:

Free flow electrophoresis

FISH:

Fluorescence in situ hybridization

FITC:

Fluorescein isothiocyanate

FRAP:

Fluorescence recovery after photobleaching

FRET:

Fluorescence resonance energy transfer

ICAT:

Isotope coded affinity tags

IEF:

Isoelectric focusing

LAMP:

Lysosome-associated membrane protein

LSCM:

Laser scanning cytometry

L-DOPA:

L-3,4-dihydroxyphenylalanine

LIF:

Laser-induced fluorescence

MALDI:

Matrix assisted laser desorption ionization

MERRF:

Myoclonus epilepsy and ragged red fiber

NAO:

10-N-nonyl acridine orange

NMR:

Nuclear magnetic resonance

PCR:

Polymerase chain reaction

PEG:

Polyethylene glycol

PMP:

Platelet-derived microparticle

PTP:

Permeability transition pore

QD:

Quantum dot

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SIMS:

Secondary ion mass spectroscopy

SR:

Sarcoplasmic reticulum

TIRFM:

Total internal reflectance microscopy

TOF:

Time-of-flight

TRITC:

Tetramethylrhodamine isothiocyanate

WFFM:

Wide field fluorescence microscopy

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Acknowledgements

Coauthors are supported by NIH grants GM008347 (K.J.O.), R01-AG20866 (H.A.), and 1K02-AG21453 (E.A.A.).

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Minnesota, 312 Church Street SE, Minneapolis, MN, 55455, USA

    Karen J. Olson & Edgar A. Arriaga

  2. Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN, 55455, USA

    Hossein Ahmadzadeh, Edgar A. Arriaga & Edgar A. Arriaga

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  1. Karen J. Olson
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  2. Hossein Ahmadzadeh
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  3. Edgar A. Arriaga
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Correspondence to Edgar A. Arriaga.

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Olson, K.J., Ahmadzadeh, H. & Arriaga, E.A. Within the cell: analytical techniques for subcellular analysis. Anal Bioanal Chem 382, 906–917 (2005). https://doi.org/10.1007/s00216-005-3135-2

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  • DOI: https://doi.org/10.1007/s00216-005-3135-2

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