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Enhancing effect of DNA on chemiluminescence from the decomposition of hydrogen peroxide catalyzed by copper(II)

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Abstract

In the absence of any special luminescence reagent, emission of weak chemiluminescence has been observed during the decomposition of hydrogen peroxide catalyzed by copper(II) in basic aqueous solution. The intensity of the chemiluminescence was greatly enhanced by addition of DNA and was strongly dependent on DNA concentration. Based on these phenomena, a flow-injection chemiluminescence method was established for determination of DNA. The chemiluminescence intensity was linear with DNA concentration in the range 2×10−7−1×10−5 g L−1 and the detection limit was 4.1×10−8 g L−1 (S/N=3). The relative standard deviation was less than 3.0% for 4×10−7 g L−1 DNA (n=11). The proposed method was satisfactorily applied for determination of DNA in synthetic samples. The possible mechanism of the CL reaction is discussed.

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Acknowledgements

The study was supported by the National Science Foundation of China (Nos. 20125514, 20175039, 50273046) and the Youth Foundation of Huo Yingdong (No. 81013).

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Authors and Affiliations

  1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, 100085, Beijing, P.R. China

    Meilin Liu & Jin-Ming Lin

  2. Department of Chemistry, School of Chemistry and Materials Sciences, Shanxi Normal University, 710062, Xi’an, P.R. China

    Baoxin Li & Zhujun Zhang

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  1. Meilin Liu
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  2. Baoxin Li
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  3. Zhujun Zhang
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  4. Jin-Ming Lin
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Correspondence to Jin-Ming Lin.

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Liu, M., Li, B., Zhang, Z. et al. Enhancing effect of DNA on chemiluminescence from the decomposition of hydrogen peroxide catalyzed by copper(II). Anal Bioanal Chem 381, 828–832 (2005). https://doi.org/10.1007/s00216-004-2922-5

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  • DOI: https://doi.org/10.1007/s00216-004-2922-5

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