Signals mediating phytoalexin (PA) production were analyzed in primary leaves of oats cv. Iowa X469 treated with an elicitor victorin. Production of the PA avenanthramide A was inhibited by DPI, an inhibitor of NADPH oxidase/nitric oxide synthase (NOS) and the NOS inhibitors l-NMMA and 1,3-PBIT. However, catalase and superoxide dismutase (SOD) hardly suppressed it. From the data, NO functions as a major reactive oxygen species in signal transduction leading to PA production in the defense response of oats. EGTA, verapamil and ruthenium red inhibited PA production, suggesting that Ca2+ influx into the cytoplasm and intracellular Ca2+ movement are involved in the defense response. Trifluoperazine, a calmodulin function inhibitor, and K-252a, a serine/threonine kinase inhibitor, also suppressed the accumulation, whereas okadaic acid, a serine/threonine phosphatase inhibitor, did not suppress it, suggesting the involvement of calmodulin and protein kinase, but not of phosphatase in PA production.
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Received 24 December 1999/ Accepted in revised form 4 February 2000
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TADA, Y., HATA, S., NAKAYASHIKI, H. et al. Signal Mediators for Phytoalexin Production in Defense Response of Oats Elicited by Victorin as a Specific Elicitor. J Gen Plant Pathol 66, 185–190 (2000). https://doi.org/10.1007/PL00012943
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DOI: https://doi.org/10.1007/PL00012943