Skip to main content
SpringerLink
Log in

Lecithin W/O microemulsions as a host for trypsin. Enzyme activity and luminescence decay studies

  • Biocolloids
  • Chapter
  • First Online:
Trends in Colloid and Interface Science X

Part of the book series: Progress in Colloid & Polymer Science ((PROGCOLLOID,volume 100))

Abstract

The possibility of using a microemulsion system based on the natural surfactant lecithin for enzymic studies was examined. Trypsin was solubilized in water-in-oil microemulsions of lecithin in isooctane and tested for activity. The enzyme activity was found higher in the microemulsion than in an aqueous solution. The “superactive” behavior was attributed to high local concentrations of the substrate, induced by the presence of the surfactant. Luminescence quenching measurements using Ru(biPy) 2+3 as a lumophore and Fe(CN) 3−6 as a quencher showed that the system is compartmentalized and that the microemulsion is percolating. The presence of trypsin influences the dispersed microenvironment by further restricting it, whereas by increasing the water content the interphase composition is altered.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Luisi PL, Straub B (ed) (1986) Reverse Micelles. Plenum Press, London

    Google Scholar 

  2. Stamatis H, Xenakis A, Kolisis F (1993) Biotech Lett 15:471

    Article  CAS  Google Scholar 

  3. Stamatis H, Xenakis A, Kolisis F (1995) An NY Acad Sci 750:237

    Article  CAS  Google Scholar 

  4. Martinek K, Levashov AV, Klyachko NL, Khmelnitski YL, Berezin YV (1986) Eur J Biochem 155:453

    Article  CAS  Google Scholar 

  5. Luisi PL, Magid L (1986) CRC Crit Rev Biochem 20:409

    Article  CAS  Google Scholar 

  6. Attwood D, Florence A (Eds) (1983) Surfactant Systems. Their chemistry, pharmacy and biology. Chapman & Hall, London

    Google Scholar 

  7. Modes S, Lianos P, Xenakis A (1990) J Phys Chem 94:3363

    Article  CAS  Google Scholar 

  8. Lianos P, Modes S, Staikos G, Brown W (1992) Langmuir 8:1054

    Article  CAS  Google Scholar 

  9. Singleton WS, Gray MS, Brown ML, White JL (1965) J Am Oil Chem Soc 42:53

    Article  CAS  Google Scholar 

  10. Grinvald A, Steinberg IZ (1974) Anal Biochem 59:583

    Article  CAS  Google Scholar 

  11. Papadimitriou V, Xenakis A, Evangelopoulos A (1993) Coloids Surf B: Biointerfaces 1:295

    Article  CAS  Google Scholar 

  12. Walde P, Peng Q, Fadnavis NW, Battistel E, Luisi PL (1988) Eur J Biochem, 173:401

    Article  CAS  Google Scholar 

  13. Peng Q, Luisi PL (1990) Eur J Biochem 188:471

    Article  CAS  Google Scholar 

  14. Lianos P, Modes S, Staikos G, Brown W (1992) Langmuir 8:1054

    Article  CAS  Google Scholar 

  15. Papoutsi D, Lianos P, Brown W (1994) Langmuir 10:3402

    Article  CAS  Google Scholar 

  16. Papadimitriou V, Xenakis A, Lianos P (1993) Langmuir 9:91

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biological Research and Biotechnology, The National Hellenic Research Foundation, 48, Vs. Constantinou Ave., 11635, Athens, Greece

    S. Avramiotis & A. Xenakis

  2. Physics Section School of Engineering, University of Patras, 26500, Patras, Greece

    S. Avramiotis & P. Lianos

Authors
  1. S. Avramiotis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Xenakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Lianos
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

C. Solans M. R. Infante M. J. García-Celma

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Dr. Dietrich Steinkopff Verlag GmbH & Co. KG

About this chapter

Cite this chapter

Avramiotis, S., Xenakis, A., Lianos, P. (1996). Lecithin W/O microemulsions as a host for trypsin. Enzyme activity and luminescence decay studies. In: Solans, C., Infante, M.R., García-Celma, M.J. (eds) Trends in Colloid and Interface Science X. Progress in Colloid & Polymer Science, vol 100. Steinkopff. https://doi.org/10.1007/BFb0115795

Download citation

  • DOI: https://doi.org/10.1007/BFb0115795

  • Published:

  • Publisher Name: Steinkopff

  • Print ISBN: 978-3-7985-1056-2

  • Online ISBN: 978-3-7985-1665-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation