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Fundamental study of degradation of dichlorodiphenyltrichloroethane in soil by laccase from white rot fungi

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Abstract

This research describes application of laccase from white-rot fungi (polyporus) to remove dichlorodiphenyltrichloroethane in soil. The degradation kinetics of dichlorodiphenyltrichloroethane in soil was also investigated by laboratory batch experiments. The results showed that laccase from white-rot fungi can effectively degrade dichlorodiphenyltrichloroethane and the degradation of total dichlorodiphenyltrichloroethane (the sum of the four dichlorodiphenyltrichloroethane compounds in a sample) was pseudo-first-order kinetics. The residues of almost all the dichlorodiphenyltrichloroethane components and total dichlorodiphenyltrichloroethane in soils treated with laccase decreased rapidly during first 15 days and then kept at a stable level during next 10 days. The residues of total dichlorodiphenyltrichloroethane in soils with different dosages laccase decreased by about 21–32 %, 29–45%, 35–51 % and 36–51 % after 5, 10, 15 and 25 days of incubation, respectively. The half-life of total dichlorodiphenyltrichloroethane in soils with different dosages laccase ranged from 24.75 to 41.75 days. The residues of total dichlorodiphenyltrichloroethane in three different types of soils decreased by 25–29 %, 39–43 %, 44–47 % and 47–52 % after 5, 10, 15 and 25 days of incubation with laccase, respectively. The half-life of total dichlorodiphenyltrichloroethane in different types of soil ranged from 24.71 to 27.68 days. The residues of total dichlorodiphenyltrichloroethane in soils with different pH levels decreased by 18–24 %, 29–39 %, 36–39 % and 39–50% after 5, 10, 15 and 25 days of incubation with laccase, respectively. The half-life of total dichlorodiphenyltrichloroethane ranged from 25.63 to 36.42 days. Laccase can be an efficient and safe agent for remediation of dichlorodiphenyltrichloroethane-contaminated soil.

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Authors and Affiliations

  1. Department of Applied Chemistry, South China Agricultural University, Guangzhou, 510642, P. R China

    Y. C. Zhao Ph.D. (Associate Professor), M. Zhang MSc., L. Liu (M.Sc. student) & W. J. Ma (M.Sc. student)

  2. College of Environmental Science and Engineering, South China University of Science & Technology, Guangzhou, 510640, P. R. China

    X. Y. Yi Ph.D. (Associate Professor)

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  1. Y. C. Zhao Ph.D.
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  4. L. Liu
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Correspondence to Y. C. Zhao Ph.D..

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Zhao, Y.C., Yi, X.Y., Zhang, M. et al. Fundamental study of degradation of dichlorodiphenyltrichloroethane in soil by laccase from white rot fungi. Int. J. Environ. Sci. Technol. 7, 359–366 (2010). https://doi.org/10.1007/BF03326145

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