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Development of EST SSRs in Finger Millet (Eleusine coracana ssp coracana) and their Transferability to Pearl Millet (Pennisetum glaucum)

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Abstract

EST-SSR markers were developed using sequence information from 1740 expressed sequence tags (ESTs) of finger millet available in the public domain. A set of 31 SSR markers were synthesized based on di, tri, tetra and penta-nucleotide repeat sequences. These were used for PCR analysis of 11 elite germplasm lines of finger millet of Indian and African origin. Out of 31 SSR markers, amplification products were obtained for 17 primer pairs. Of these nine were found polymorphic with two alleles per locus. These 17 SSR primer pairs were also tested for amplification in three varieties of pearl millet (Pennisetum glaucum) and 11 could be transferred to pearl millet. The informative EST SSR markers developed, can be used in finger millet as well as pearl millet genetic improvement projects.

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Abbreviations

ESTs:

Expressed sequence tags

SSRs:

Simple sequence repeats

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Authors and Affiliations

  1. National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa campus, New Delhi, 110 012, India

    Lalit Arya, Manjusha Verma, V. K. Gupta & J. L. Karihaloo

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  1. Lalit Arya
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  2. Manjusha Verma
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  3. V. K. Gupta
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  4. J. L. Karihaloo
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Correspondence to Lalit Arya.

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Arya, L., Verma, M., Gupta, V.K. et al. Development of EST SSRs in Finger Millet (Eleusine coracana ssp coracana) and their Transferability to Pearl Millet (Pennisetum glaucum). J. Plant Biochem. Biotechnol. 18, 97–100 (2009). https://doi.org/10.1007/BF03263303

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  • DOI: https://doi.org/10.1007/BF03263303

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