Skip to main content
SpringerLink
Log in

A spectroscopic study on the interaction between ferric oxide nanoparticles and human hemoglobin

  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

Magnetic nanoparticles can promote many attractive functions in biomedicine, which may contribute to the prevention of human disease but also may be potentially harmful. In the present study, the interaction of Fe2O3 nanoparticles with human hemoglobin (Hb) was studied by fluorescence, circular dichroism and UV/vis spectroscopies. Fluorescence data revealed that the fluorescence quenching of Hb by Fe2O3 nanoparticles was the result of the formed complex of Fe2O3 nanoparticles-Hb. Binding constants and other thermodynamic parameters were determined at three different temperatures. The hydrophobic interactions are the predominant intermolecular forces to stabilize the complex. Circular dichroism studies did not show any changes in the content of secondary structure of hemoglobin after Fe2O3 nanoparticles treatment. This study provides important insight into the interaction of Fe2O3 nanoparticles with hemoglobin, which may be a useful guideline for further using of these nanoparticles in biomedical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A.S.G. Curtis, C. Wilkinson, Trends Biotechnol. 19 (2001) 97.

    CAS  Google Scholar 

  2. S.M. Moghimi, A.C.H. Hunter, J.C. Murray, Pharm. Rev. 53 (2001) 283.

    CAS  Google Scholar 

  3. J. Panyam, V. Labhasetwar, Adv. Drug. Del. Rev. 55 (2003) 329.

    CAS  Google Scholar 

  4. J.M. Wilkinson, Med. Dev. Technol. 14 (2003) 29.

    CAS  Google Scholar 

  5. D. Sellmyer, R. Skomski, Advanced magnetic nanoparticles, Springer Science, New York, 2006.

    Google Scholar 

  6. A.S. Arbab, L.A. Bashaw, B.R. Miller, E.K. Jordan, B.K. Lewis, H. Kalish, J.A. Frank, Radio. 229 (2003) 838.

    Google Scholar 

  7. C.C. Berry, A.S.G. Curtis, J. Phys. D: Appl. Phys. 36 (2003)198.

    Google Scholar 

  8. U. Häfeli, W. Schütt, J. Teller, M. Zborowski, Scientific and clinical applications of magnetic carriers, Plenum Press, New York, 1997.

    Google Scholar 

  9. Q.A. Pankhurst, J. Connolly, S.K. Jones, J. Dobson, J. Phys.D: Appl. Phys. 36 (2003)167.

    Google Scholar 

  10. P. Reimer, R. Weissleder, Radio. 36 (1996) 153.

    CAS  Google Scholar 

  11. E.X. Wu, H. Tang, K.K. Wong, J. Wang, J. Magn. Reson. Imag. 19 (2004) 50.

    Google Scholar 

  12. A. K. Gupta, M. Gupta, Biomaterials 26 (2005) 3995.

    CAS  Google Scholar 

  13. H.M. Johng, J.S. Yoo, T.J. Yoon, H.S. Shin, B.C. Lee, C. Lee, J.K. Lee, K.S. Soh, Evid. Based Complement Alternat. Med. 4 (2007) 77.

    Google Scholar 

  14. Y.Q. Wanga, H.M. Zhangb, G.C. Zhanga, S.X. Liub, Q.H. Zhoub, Z.H. Feia, Z.T. Liua, Int. J. Biol. Macromol. 41(2007) 243.

    Google Scholar 

  15. M.F. Perutz, Scientific American, vol. 6, 2005.

    Google Scholar 

  16. G. Weber, Adc. Protein Chem. 8(1953) 415.

    CAS  Google Scholar 

  17. F.W. Teale, J. Biochim. Biophys. Acta 35 (1959) 543.

    CAS  Google Scholar 

  18. B. Sengupta, P.K. Sengupta, Biochem. Biophys. Res. Commun. 299 (2002) 400.

    CAS  Google Scholar 

  19. W. Sun, D. Wang, Z. Zhai, R. Gao and K. Jiao, J. Iran. Chem. Soc. 6 (2009) 412.

    Article  CAS  Google Scholar 

  20. F.W.J. Teale, G. Weber, Biochem. J. 72 (1959) 156.

    Google Scholar 

  21. B. Alpert, D.M. Jameson, G. Weber, J. Photochem. Photobiol. 31(1980) 1.

    CAS  Google Scholar 

  22. R.A. Goldbeck, R.M. Esquerra, D.S. Kliger, J. Americ. Chem. Soc. 124 (2002) 7646.

    CAS  Google Scholar 

  23. S. Venkateshrao, P.T. Manoharan, Spectrochim. Acta Part A 60 (2004) 2523.

    CAS  Google Scholar 

  24. J. Zhoua, X. Wua, X. Gua, L. Zhoub, K. Songa, S. Weia, Y. Fenga, J. Shena, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr. 72 (2009) 151.

    Google Scholar 

  25. M. Jiang, M.X. Xie, D. Zheng, Y. Liu, X.Y. Li, X. Chen, J. Mol. Struct. 692 (2004) 70.

    Google Scholar 

  26. J.Q. Lu, F. Jin, T.Q. Sun, X.W. Zhou, Int. J. Biol. Macromol. 40 (2007) 299.

    CAS  Google Scholar 

  27. Y.J. Hu, Y. Liu, X.S. Shen, X.Y. Fang, S.S. Qu, J. Mol. Struct. 738 (2005) 143.

    CAS  Google Scholar 

  28. P. Atkins, J. De Paula, Physical Chemistry, 8th Ed., W.H. Freeman and Company, 2006, pp. 212–220.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    S. Zolghadri, A. A. Saboury, E. Amin & A. A. Moosavi-Movahedi

Authors
  1. S. Zolghadri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Saboury
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Amin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Moosavi-Movahedi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. A. Saboury.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zolghadri, S., Saboury, A.A., Amin, E. et al. A spectroscopic study on the interaction between ferric oxide nanoparticles and human hemoglobin. JICS 7 (Suppl 2), S145–S153 (2010). https://doi.org/10.1007/BF03246193

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03246193

Keywords

Search

Navigation