Abstract
In this study, methotrexate (MTX)-encapsulated polymeric micelles using methoxy poly(ethylene glycol) (MPEG)-grafted chitosan (ChitoPEG) copolymer were prepared. The MTX-incorporated polymeric micelles of ChitoPEG copolymer has a particle size of around 50-100 nm. In 1H nuclear magnetic resonance (NMR) study, the specific peaks of MTX disappeared in heavy water (D2O) and only the specific peak of MPEG was observed, while all of the peaks were confirmed in dimethyl sulfoxide (DMSO). These results indicated that MTX was complexed with chitosan and then formed an ion complex inner-core of the polymeric micelle in an aqueous environment. The drug contents of the polymeric micelle were around 4∼12% and the loading efficiency of MTX in the polymeric micelles was higher than 60% (w/w) for all of the formulations. The cytotoxicity of MTX and MTX-incorporated polymeric micelle against CT26 tumor cells was not significantly changed.
Similar content being viewed by others
References
S. Hirano,Polym. Int.,48, 732 (1999).
J. M. Dang and K. W. Leong,Adv. Drug Del. Rev.,58, 487 (2006).
M. Thanou, J. C. Verhoef, and H. E. Junginger,Adv. Drug Del. Rev.,52, 117 (2001).
T. W. Chung, J. Yang, T. Akaike, K. Y. Cho, J. W. Nah, S. I. Kim, and C. S. Cho,Biomaterials,23, 2827 (2002).
S. Y. Chae, M. K. Jang, and J. W. Nah,J. Control. Release,102, 383 (2005).
S. Y. Chae, S. Son, M. Lee, M. K. Jang, and J. W. Nah,J. Control. Release,109, 330 (2005).
P. Calvo, C. Remuñan-López, J. L. Vila-Jato, and M. J. Alonso,Pharm. Res.,14, 1431 (1997).
D. G. Kim, C. Choi, Y. I. Jeong, M. K. Jang, J. W. Nah, S. K. Kang, and M. S. Bang,Macromol. Res.,14, 66 (2006).
K. Kataoka, A. Haradaa, and Y. Nagasaki,Adv. Drug Del. Rev.,47, 113 (2001).
N. Nishiyama and K. Kataoka,Adv. Exp. Med. Biol.,519, 155 (2003).
D. G. Kim, Y. I. Jeong, and J. W. Nah,J. Appl. Polym. Sci.,105, 3246 (2007).
A. F. Thünemann, J. Beyermann, and H. Kukula,Macromolecules,33, 5906 (2000).
P. Calabresi and R. E. Parks,The Pharmacological Basis of Therapeutics, Macmillan, New York, 1975, p. 1254–1307.
P. Calabresi, B. A. Chabner, L. S. Goodman, and A. Gilman, Eds.,in The Pharmacological Basis of Therapeutics, Macmillan, New York, 1991, p.1243–1247.
Y. Li and G. S. Kwon,Pharm. Res.,17, 607 (2000).
Y. Li and G. S. Kwon,Colloid Surface B,16, 217 (1999).
H. Kang, J. D. Kim, S. H. Han, and I. S. Chang,J. Control. Release,81, 135 (2002).
Y. Zhang and R. X. Zhuo,Biomaterials,26, 2089 (2005).
D. Nevozhay, R. Budzynska, U. Kanska, M. Jagiello, M. S. Omar, J. Boratynski, and A. Opolski,Anticancer Res.,26, 1135 (2006).
X. Yang, Q. Zhang, Y. Wang, H. Chen, H. Zhang, F. Gao, and L. Liu,Colloid Surface B,61, 125 (2008).
Y. Mizumura, Y. Matsumura, T. Hamaguchi, N. Nishiyama, K. Kataoka, T. Kawaguchi, W. J. Hrushesky, F. Moriyasu, and T. Kakizoe,Jpn. J. Cancer Res.,92, 328 (2001).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jeong, YI., Seo, DH., Kim, DG. et al. Methotrexate-incorporated polymeric micelles composed of methoxy poly(ethylene glycol)-grafted chitosan. Macromol. Res. 17, 538–543 (2009). https://doi.org/10.1007/BF03218904
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03218904